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Plix 402

Manufactured by Thermo Fisher Scientific
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The PLIX_402 is a laboratory instrument designed for the analysis of samples. It utilizes advanced technology to provide accurate and reliable measurements. The core function of the PLIX_402 is to facilitate scientific research and data collection.

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Lab products found in correlation

Gateway recombination technology, by Thermo Fisher Scientific (2 mentions) Plko 1 shslfn5 plasmids rhs4533 eg162394, by Horizon Discovery (2 mentions)

2 protocols using plix 402

1

Plasmid Construction and Mutagenesis of SLFN5

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pcDNA6.2 plasmids expressing C-terminal V5-tagged SLFN5 and SLFN11 were kindly provided by Sara L. Sawyer (University of Colorado Boulder). Plasmids expressing GFP-SLFN5 (pcDNA6.2-/N-EmGFP-DEST), His-SLFN5 (pDEST17), Adenoviral SLFN5-V5 (pAd/CMV/V5-DEST), and SLFN5-HA under a tetracycline-inducible promoter (pLIX_402, gifted from David Root, Addgene plasmid # 41394) were created using Gateway recombination technology (Invitrogen). Point and deletion mutants of SLFN5 were generated using the Stratagene QuickChange Site-Directed Mutagenesis protocol. pLKO.1-shSLFN5 plasmids (RHS4533-EG162394) were purchased from Dharmacon. pX330-GFP-Cas9 plasmid for human SLFN5 knockout was constructed with single guide RNA (5’-GATGCAGGAAAAGTCACCCT-3’).
Kim E.T., Dybas J.M., Kulej K., Reyes E.D., Price A.M., Akhtar L.N., Orr A., Garcia B.A., Boutell C, & Weitzman M.D. (2021). Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription. Nature microbiology, 6(2), 234-245.
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2

Plasmid Construction and Mutagenesis of SLFN5

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pcDNA6.2 plasmids expressing C-terminal V5-tagged SLFN5 and SLFN11 were kindly provided by Sara L. Sawyer (University of Colorado Boulder). Plasmids expressing GFP-SLFN5 (pcDNA6.2-/N-EmGFP-DEST), His-SLFN5 (pDEST17), Adenoviral SLFN5-V5 (pAd/CMV/V5-DEST), and SLFN5-HA under a tetracycline-inducible promoter (pLIX_402, gifted from David Root, Addgene plasmid # 41394) were created using Gateway recombination technology (Invitrogen). Point and deletion mutants of SLFN5 were generated using the Stratagene QuickChange Site-Directed Mutagenesis protocol. pLKO.1-shSLFN5 plasmids (RHS4533-EG162394) were purchased from Dharmacon. pX330-GFP-Cas9 plasmid for human SLFN5 knockout was constructed with single guide RNA (5’-GATGCAGGAAAAGTCACCCT-3’).
Kim E.T., Dybas J.M., Kulej K., Reyes E.D., Price A.M., Akhtar L.N., Orr A., Garcia B.A., Boutell C, & Weitzman M.D. (2021). Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription. Nature microbiology, 6(2), 234-245.
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