The human normal glial cell line HEB and glioma cell lines U251, U87, T98-G and A172 were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. DMEM/HG, fetal bovine serum (FBS), Opti-MEM and 0.25% trypsin containing 0.02% EDTA were purchased from Gibco, MTT kit, 5-aza-2'-deoxycytosine (AZA) and histone deacetylase inhibitor (TSA) from Sigma, Trizol, reverse transcription kit from Thermo, SYBR Green Real-time PCR kit from Shanghai Solarbio Bioscience & Technology, QIAamp DNA Mini kit and EpiTect Bisul te kit from Qiagen, Lipofectamine 3000 from Invitrogen, and the cell cycle assay kit from BD. The pcDNA3.1-miR-133a mimic, scrambled miRNA (miR-NC) and the pcDNA3.1-PPARγ overexpression plasmid were obtained from Guangzhou Ruibo Bio. Antibodies against PPARγ, cyclin D1, cyclin D2, CDK4 and β-actin were from Abcam, and the horseradish peroxidase (HRP)-labeled IgG secondary antibody from Guangzhou Jingcai. PPARγ wild type (WT) and mutant (MUT) luciferase reporter plasmids were purchased from Shanghai Jima and the luciferase assay kit from Promega. The PCR primers were synthesized by Shanghai Shenggong. Other reagents were from our laboratory and of analytical grade.
The cell cycle assay kit
Manufactured by BD
The cell cycle assay kit is a laboratory tool designed to analyze the progression of cells through the different phases of the cell cycle. It provides the necessary reagents and protocols to measure the distribution of cells in the G1, S, and G2/M phases.
Automatically generated - may contain errors
Lab products found in correlation
Reverse transcription kit, by Thermo Fisher Scientific (9 mentions)
Trypsin, by Thermo Fisher Scientific (3 mentions)
Dmem hg, by Thermo Fisher Scientific (3 mentions)
Mtt kit, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions)
Opti mem, by Thermo Fisher Scientific (3 mentions)
Qiaamp dna mini kit, by Qiagen (3 mentions)
Sybr green real time pcr kit, by Solarbio (3 mentions)
Luciferase assay kit, by Promega (2 mentions)
Epitect bisul te kit, by Qiagen (2 mentions)
5 aza 2 deoxycytosine aza and histone deacetylase inhibitor tsa, by Merck Group (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Epitect bisulfite kit, by Qiagen (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay kit, by Promega (1 mentions)
Pmirglo vector, by Promega (1 mentions)
3 protocols using the cell cycle assay kit
1
Molecular Mechanisms of miR-133a in Glioma Regulation
2
MicroRNA-133a Regulates Glioma Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human normal glial cell line HEB and glioma cell lines U251, U87, T98-G and A172 were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. DMEM/HG, fetal bovine serum (FBS), Opti-MEM and 0.25% trypsin containing 0.02% EDTA were purchased from Gibco, MTT kit, 5-Azacytidine (AZA) and histone deacetylase inhibitor (TSA) from Sigma, Trizol, reverse transcription kit from Thermo, SYBR Green Real-time PCR kit from Shanghai Solarbio Bioscience & Technology, QIAamp DNA Mini kit and EpiTect Bisulfite kit from Qiagen, Lipofectamine 3000 from Invitrogen, and the cell cycle assay kit from BD. The pcDNA3.1-miR-133a mimic, scrambled miRNA (miR-NC) and the pcDNA3.1-PPARG overexpression plasmid were obtained from Guangzhou Ruibo Bio. Antibodies against PPARG, cyclin D1, cyclin D2, CDK4 and 𝛽-actin were from Abcam, and the horseradish peroxidase (HRP)-labeled IgG secondary antibody from Guangzhou Jingcai. PPARG wild type (WT) and mutant (MUT) luciferase reporter plasmids were purchased from Shanghai Jima and the luciferase assay kit from Promega. The PCR primers were designed and synthesized by Shanghai Sangon Biotechnology. Other reagents were from our laboratory and of analytical grade.
3
Insights into Glioma Cell Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human normal glial cell line HEB and glioma cell lines U251, U87, T98-G and A172 were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. DMEM/HG, fetal bovine serum (FBS), Opti-MEM and 0.25% trypsin containing 0.02% EDTA were purchased from Gibco, MTT kit, 5-aza-2'-deoxycytosine (AZA) and histone deacetylase inhibitor (TSA) from Sigma, Trizol, reverse transcription kit from Thermo, SYBR Green Real-time PCR kit from Shanghai Solarbio Bioscience & Technology, QIAamp DNA Mini kit and EpiTect Bisul te kit from Qiagen, Lipofectamine 3000 from Invitrogen, and the cell cycle assay kit from BD. The pcDNA3.1-miR-133a-5p mimic, scrambled miRNA (miR-NC) and the pcDNA3.1 PGC-1α overexpression plasmid were obtained from Guangzhou Ruibo Bio. Antibodies against PGC-1α, cyclin D1, cyclin D2, Cdk4 and β-actin were from Abcam, and the horseradish peroxidase (HRP)-labeled IgG secondary antibody from Guangzhou Jingcai. QuickChange Site-Directed Mutagenesis kit was purchased from Stratagene. Dual-Luciferase Reporter Assay kit and pmirGLO vector were from Promega. The PCR primers were synthesized by Shanghai Shenggong. Other reagents were from our laboratory and of analytical grade.
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