The 6-gingerol and curcumin contents were determined in the phenolic extracts of respectively ginger-and turmeric-enriched soybean oils by HPLC using a Thermo Finnigan SpectraSystem UV6000LP HPLC system (Thermo Finnigan, San Jose, CA, USA) with diode-array detection and a Supelcosil LC-18 column (Sigma-Aldrich). Before their injection into the column, samples were filtered through 0.22 μm Millipore cellulose acetate filters (Merck Millipore, Billerica, MA, USA). Regarding 6-gingerol, the HPLC operating parameters were according to the slightly modified method of Hu et al. ( 2011
Millipore cellulose acetate filters
Manufactured by Merck Group
Sourced in United States
Millipore cellulose acetate filters are laboratory equipment used for filtration and separation processes. They are made of cellulose acetate material and are designed to remove particles, microorganisms, and other contaminants from liquid samples.
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2 protocols using millipore cellulose acetate filters
1
Quantification of Ginger and Turmeric Compounds in Soybean Oil
2
Quantification of Ginger and Curcumin in Oils
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The 6-gingerol, 6-shogaol and curcumin were HPLC-quantified in the phenolic extracts of oil samples through a Thermo Finnigan SpectraSystem UV6000LP HPLC system (Thermo Finnigan, San Jose, CA, USA) with diode-array detector and a Supelcosil LC-18 column (Sigma-Aldrich). The samples were injected into the column after filtering with 0.22 µm Millipore cellulose acetate filters (Merck Millipore, Billerica, MA, USA).
The 6-gingerol and 6-shogaol were HPLC-detected as follows [31] (link) C; chromatographic run time of 70 min. The content of each compound was expressed as milligram per gram of oil (mg/g).
The 6-gingerol and 6-shogaol were HPLC-detected as follows [31] (link) C; chromatographic run time of 70 min. The content of each compound was expressed as milligram per gram of oil (mg/g).
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