Ft ir spectrometer

All reactions were carried out with the exclusion of moisture. All solvents were dried. All melting points were uncorrected. The IR spectra were recorded as potassium bromide pellets on an Aldrich FT-IR spectrometer (Central lab at Faculty of Science, Benha, Ain Shams, and Cairo universities). Mass spectra were recorded using GCMS (gas chromatography-mass spectrometry) on the Shimadzu Q.P.-2010 Plus (Microanalytical center, Ain shams University), using UV light. Spectrometer spins on a Bruker DPX 400 MHz Spectro spins were used to record the ¹HNMR (500 MHz, DMSO-d₆) and ¹³CNMR (125 MHz, Chloroform-d) spectra. Chemical shift (d) values were stated in parts per million (ppm) using internal standard tetramethylsilane. The D₂O exchange confirmed the exchangeable protons (OH and NH). LC-MS/MS (PerkinElmer) was used to record the mass spectra, presented as m/z. Elemental analyses were achieved at Ain Shams University on an elementary analysis system by using a PerkinElmer 240 analyzer. The purity of synthesized compounds, as well as the progress of the reaction, was assessed by ascending thin-layer chromatography (TLC) (silica gel Fluka, 706, 43–50 E.A.) by using the methanol/chloroform (9:1 v/v) and methylene chloride/chloroform (4:1 v/v) combination as the solvent system.

All reactions were performed, excluding moisture, dried solvents, and uncorrected melting points. The IR spectra were recorded as potassium bromide pallets on an Aldrich FT-IR spectrometer (Central Laboratory at Faculty of Science, Benha, Ain Shams, and Cairo Universities). Mass spectra were recorded on GCMS (gas chromatography–mass spectrometer) Shimadzu QP- 2010 Plus (Microanalytical center, Ain shams University). Elemental analysis was determined using UV light on the Ain Shams University elementary analysis system. The Bruker Spectro spin DPX-400MHz was used to record the ¹H NMR (500 MHz, DMSO-d₆) and ¹³C NMR (125 MHz, Chloroform-d) spectra. Chemical shift (d) values were stated in parts per million (ppm) using internal standard tetramethylsilane. The D₂O exchange confirmed that the exchangeable protons (OH and NH) and some other labial hydrogens are exchangeable. LC–MS/MS (PerkinElmer) was used to record the mass spectra, presented as m/z. Elemental analysis was achieved using the PerkinElmer 240 analyzer. The purity of synthesized compounds and the progress of the reaction were evaluated by ascending thin layer chromatography (TLC) (silica gel Fluka, 706, 43-50 EA) using methanol/chloroform (9:1 v/v) and methylene chloride/chloroform (4:1 v/v) as a solvent system.