Rabbit anti-human perilipin-3 antibody was raised against a peptide of human perilipin-3 segment (amino acids 305–318)47 (link). Rabbit anti-CCTα antibody was donated by Dr. Neale Ridgway (Dalhousie University). Rabbit anti-lamin B receptor (Genway Biotech GWB-C7CA28), rabbit anti-lamin B1 (Abcam ab16048), mouse anti-lamin A/C (Cell Signaling #4777), rabbit anti-actin (Sigma A2066), and mouse anti-FLAG (Sigma F1804), goat anti-MTP (Santa Cruz sc-33116), mouse anti-ApoE (Immunogenetics M-012-0500), mouse anti-choline kinase β (Santa Cruz sc-398957), mouse anti-diacylglycerol cholinetransferase 1 (Santa Cruz sc-515577), rabbit anti-choline/ethanolamine phosphotransferase 1 (Abgent #AP10372a), goat anti-ApoB (Rockland 600-101-111), rabbit anti-ApoC-III (Thermo Fisher 6H21L11), mouse anti-V5 (Thermo Fisher R960-25), mouse anti-choline kinase α (CKα) (Proteintech 13520-1-AP), guinea pig anti-perilipin-3 (Progen GP30), and mouse anti-nucleopore complex (Covance MMS-120P) antibodies were obtained from the respective suppliers. Secondary antibodies conjugated to fluorochromes and peroxidase were purchased from Thermo Fisher, Jackson ImmunoResearch Lab, and Bethyl Laboratories. See Supplementary Table 1 for dilutions of antibodies used for immunofluorescence labeling and Western blotting.
Mms 120p
Manufactured by Fortrea
The MMS-120P is a compact and versatile laboratory equipment designed for a variety of scientific applications. It features a rugged construction and advanced capabilities to meet the demands of modern research and testing environments. The core function of the MMS-120P is to provide accurate and reliable measurements with its high-precision sensors and intuitive user interface.
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Lab products found in correlation
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
Rabbit anti actin, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Mouse anti lamin a c, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Ab26300, by Abcam (1 mentions)
Ab2792, by Abcam (1 mentions)
Ab133256, by Abcam (1 mentions)
Nb100 304, by Novus Biologicals (1 mentions)
Sc 8035, by Santa Cruz Biotechnology (1 mentions)
2 protocols using mms 120p
1
Antibodies Used in Lipid Metabolism Research
2
Comprehensive Antibody Characterization Protocol
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Commercially obtained antibodies included anti-LAP1 (LS-C288839; LifeSpan Biosciences; dilution 1/2000 WB, 1/100 IF), anti-emerin (PA5-29731; Thermo Fisher Scientific; dilution 1/2000 WB, 1/400 IF), anti-lamin A (ab26300; Abcam; dilution 1/1000), anti-PDI (ab2792; Abcam; dilution 1/100), anti-LULL1 (HPA051849; Sigma-Aldrich; dilution 1/200), anti-tubulin (sc-8035, Santa Cruz Biotechnology; dilution 1/5000), anti-lamin A/C (ab133256, Abcam; dilution 1/10000 WB, 1/500 IF), anti-nuclear pore complex proteins mAb414 (MMS-120P; Covance; dilution 1/300), anti-cleaved caspase 3 (Asp175, #9661; Cell Signaling; dilution 1/400), anti-53BP1 (NB100-304; Novus Biologicals; dilution 1/1000), and Alexa Fluor® 488 anti-H2A.X Phospho (Ser139, #613405; BioLegend; dilution 1/200). Secondary antibodies for indirect immunefluorescence staining were TRITC goat anti-rabbit (111-025-003; Jackson ImmunoResearch; dilution 1/300) and Alexa Fluor 488-donkey anti–mouse IgG (715-545-150; Jackson ImmunoResearch; dilution 1/300). Affinity purified anti-torsinA polyclonal antibody was a kind gift from Christian Schlieker (Yale University, New Haven, CT) and was used at a dilution of 1/2000.
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