Polarstar omega microtitre plate reader

UV-visible spectral analysis was used to monitor the bio-reduction of the Ag salt solution of the aqueous extract of Annona muricata leaves. The synthesis was monitored periodically by recording the absorbance spectral using a Polar Star Omega Microtitre Plate reader (BMG Labtech, Ortenberg, Germany) at the wavelength ranging from 200 to 700 nm. This was followed by subjecting the AgNPs to Fourier Transform Infrared Spectroscopy (FTIR) to determine functional groups that contributed to the synthesis at wavenumber ranging from 4000 cm⁻¹ to 400 cm⁻¹ using the Thermo Scientific TM Nicolet. X-ray diffraction (XRD) nature was carried on the particles to determine the crystal phase using the advanced model Bruker AXS D8, operated at a voltage of 40 kV and a current of 30 mA with Cu, Kα radiation (λ- 1.54056 Å) in a Ɵ-2Ɵ configuration with the range of 20–80^o. TEM analysis was done on the TEM JEOL at 300 kV to determine the size and shape of the silver nanoparticles from the TEM micrograph. The elemental analysis of the nanoparticles was assessed using Energy Dispersive X-Ray Spectroscopy (EDS). Dynamic light scattering (DLS) of the nanoparticles was determined using a Malvern Zetasizer Nanoseries (Nano-ZS90) to study the phase behaviour of the particles.