Lysoblue

Manufactured by Keygen Biotech

Sourced in China

LysoBlue is a fluorescent dye used for the detection and visualization of lysosomes in live cells. It selectively stains acidic organelles such as lysosomes, allowing researchers to study their distribution and dynamics within the cellular environment.

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Lab products found in correlation

Sp5ii, by Leica (1 mentions)

2 protocols using lysoblue

Nanovaccine Uptake and Lysosome Escape

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To examine the cellular uptake of nanovaccines, immature BMDCs (5 × 10⁵ cells per well) were seeded in confocal dishes and stimulated with BN@HM-OVA-FITC (FITC-labeled OVA, 25 μg/mL) for 2 and 6 h in medium with various pH values (7.4 and 6.8). After washing the spare nanovaccine, BMDCs were harvested and then labeled with Dil to quench the extracellular fluorescence. After washing three times, the cellular internalization of nanovaccine was obtained utilizing a confocal laser scanning microscope (CLSM) (Leica SP5II, Germany).
The lysosome escape behavior of nanovaccine was investigated by CLSM. Immature BMDCs (1 × 10⁶ cells per well) were plated in confocal dishes and then incubated with BN@HM-OVA-FITC (FITC-labeled OVA, 25 μg/mL) in medium with various pH values (7.4 and 6.8). After the designated incubation time points (6 and 36 h), BMDCs labeled with Dil were washed with PBS and then stained with LysoBlue (KeyGEN BioTECH, China). The co-localization degree of lysosomes was determined utilizing a confocal laser scanning microscope (Leica SP5II, Germany).

Xie X., Feng Y., Zhang H., Su Q., Song T., Yang G., Li N., Wei X., Li T., Qin X., Li S., Wu C., Zhang X., Wang G., Liu Y, & Yang H. (2022). Remodeling tumor immunosuppressive microenvironment via a novel bioactive nanovaccines potentiates the efficacy of cancer immunotherapy. Bioactive Materials, 16, 107-119.

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Evaluating Lysosomal Escape of Nanomaterials

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FITC and DiD are loaded into the MPD to assess lysosomal escape. MLE-12 cells were pretreated with TNF-α (10 ng/mL) and IFN-γ (10 ng/mL) for 24 h. The MLE-12 cells were incubated with MSCM/PLGA/DiD/FITC or MPD/DiD/FITC for 4 h and 12 h. Next, the cells were washed three times and stained with LysoBlue (KeyGEN BioTECH, China) for 60 min. The cells were washed with PBS and imaged under CLSM (LSM880, Zeiss, Germany).

Qin A., Chen S., Li S., Li Q., Huang X., Xia L., Lin Y., Shen A., Xiang A.P, & Zhang L. (2022). Artificial stem cells mediated inflammation-tropic delivery of antiviral drugs for pneumonia treatment. Journal of Nanobiotechnology, 20, 335.

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