HCC cell lines SNU-449, SNU-182, Huh7, LM3, Bel-7405, SK-hep1, Hep3B and normal human liver cell line L02 were purchased from the American Type Culture Collection (Manassas, VA, USA) or Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). SNU-449, SK-hep1 and SNU-182 were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco). The other cells were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) (Gibco) containing 10% FBS. Penicillin (100 U/ml) and streptomycin (100 μg/ml) were supplemented to the medium to reduce the chance of microbial contamination. All the cells were maintained in a humidified atmosphere at 37 °C with 5% CO2/95% air.
Bel 7405
Manufactured by American Type Culture Collection
Sourced in United States
The Bel-7405 is a laboratory equipment product offered by American Type Culture Collection. It is used for the cultivation and maintenance of cell cultures. The core function of the Bel-7405 is to provide a controlled environment for the growth and propagation of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Sk hep1, by American Type Culture Collection (3 mentions)
Snu 449, by American Type Culture Collection (3 mentions)
Hep3b, by American Type Culture Collection (3 mentions)
Snu 182, by American Type Culture Collection (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Snu449, by Thermo Fisher Scientific (2 mentions)
Sk hep1, by Thermo Fisher Scientific (2 mentions)
Snu 182, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hcclm3, by American Type Culture Collection (2 mentions)
Dulbecco s modified eagle medium dmem high glucose, by Thermo Fisher Scientific (1 mentions)
High glucose dulbecco s modi ed eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Mhcc97h, by American Type Culture Collection (1 mentions)
4 protocols using bel 7405
1
Cultivation of Hepatocellular Carcinoma Cell Lines
2
Culturing HL-7701 and HCC Cell Lines
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HL-7701 cells and HCC cell lines (Bel-7405, HCCLM3, Huh7, and Hep3B) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS; Gibco, cat. no. 10099–141) at 37°C in a 5% CO2 atmosphere. Cells in logarithmic growth phase were used for all experiments.
3
Establishment and Characterization of HCC Cell Lines
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A total of 74 pairs of HCC cancerous and corresponding non-cancerous tissues were gathered from HCC patients who underwent surgery from 2017 to 2020 at The First A liated Hospital of Guangxi Medical University. These tissue samples were immediately transferred into liquid nitrogen right after the surgery and then stored at -80 ºC for future use. This study was reviewed and approved by the ethics committee of The First A liated Hospital of Guangxi Medical University, and written informed consent in accordance with the Declaration of Helsinki and its later revision was provided by all the patients.
Cell lines and cell culture HCC cell lines SNU-449, SNU-182, Huh7, LM3, Bel-7405, SK-hep1, Hep3B and normal human liver cell line L02 were purchased from the American Type Culture Collection (Manassas, VA, USA) or Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). SNU-449, SK-hep1 and SNU-182 were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco). The other cells were cultured in high-glucose Dulbecco's modi ed Eagle medium (DMEM) (Gibco) containing 10% FBS. Penicillin (100 U/ml) and streptomycin (100 μg/ml) were supplemented to the medium to reduce the chance of microbial contamination. All the cells were maintained in a humidi ed atmosphere at 37℃ with 5% CO 2 /95% air.
Cell lines and cell culture HCC cell lines SNU-449, SNU-182, Huh7, LM3, Bel-7405, SK-hep1, Hep3B and normal human liver cell line L02 were purchased from the American Type Culture Collection (Manassas, VA, USA) or Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). SNU-449, SK-hep1 and SNU-182 were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco). The other cells were cultured in high-glucose Dulbecco's modi ed Eagle medium (DMEM) (Gibco) containing 10% FBS. Penicillin (100 U/ml) and streptomycin (100 μg/ml) were supplemented to the medium to reduce the chance of microbial contamination. All the cells were maintained in a humidi ed atmosphere at 37℃ with 5% CO 2 /95% air.
4
Culturing Human Hepatocyte and HCC Cell Lines
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Standard human hepatocytes THLE-3 and HCC cell lines MHCC-97H, Huh7, HCC-LM3, Bel-7405, SNU-449, SK-Hep-1, and SNU-182 were purchased from the American Type Culture Collection or the Cell Bank of the National Collection of Authenticated Cell Cultures (Shanghai, China). The conventional culture of HCC cell lines was performed (Li et al., 2015a (link)). Cells were cultured in appropriate culture media containing 10% fetal bovine serum (FBS; Gibco) supplemented with penicillin (100 U/ml) and streptomycin (100 μg/ml). All cell lines were cultured at 37°C in an incubator using 5% CO2/95% air and 100% humidity to minimize the chances of bacterial contamination. Cells were passaged every 1–2 days to maintain logarithmic growth.
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