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Jiyoye

Manufactured by American Type Culture Collection
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The Jiyoye is a laboratory equipment designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The core function of the Jiyoye is to maintain consistent temperature, humidity, and gas composition to support optimal cell growth and proliferation.

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Lab products found in correlation

Rpmi 1640 media, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Suit 2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Geneticin selective antibiotic, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Panc 1, by American Type Culture Collection (1 mentions) Aspc 1, by American Type Culture Collection (1 mentions) Antibiotic solution, by Merck Group (1 mentions) Caki 1, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hev0011, by RIKEN BioResource Center (1 mentions) Cd3 apc, by BioLegend (1 mentions) Cd8 fitc, by BioLegend (1 mentions) Cd3 apc cy7 clone hit3a, by BioLegend (1 mentions) Cd4 pe cy7 clone rpa t4, by BioLegend (1 mentions)

4 protocols using jiyoye

1

Detailed Protocol for Target Cell Selection

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T2A24 (HLA-A*02:01, HLA-A*24:02, lymphoblast) cells were provided by Dr. Kuzushima (Aichi Cancer Center) [39 (link)]. Jiyoye (HLA-A32, B17, Bw37, Burkitt’s lymphoma), EB-3 (HLA-A3, Aw32, Cw2, Burkitt’s lymphoma), PANC-1 (pancreatic cancer), and ASPC1 (pancreatic cancer) cells were purchased from the American Type Culture Collection (Manassas, VA). T2A24 cells were maintained in RPMI1640 media with HEPES (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Biowest, France) and 0.8 mg/ml Geneticin® Selective Antibiotic (Thermo Fisher Scientific, Waltham, MA). Jiyoye and EB-3 cells were maintained in RPMI1640 media (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (liquid, Thermo Fisher Scientific, Waltham, MA). KP2, KP3, KP4, and SUIT2 cells were purchased from the JCRB Cell Bank (Japan). The expression levels of HLA-A*2402 and mesothelin were examined by flow cytometry with an anti-HLA-A*2402 monoclonal antibody and anti-mesothelin antibody to select HLA-A*2402- and mesothelin-positive target cancer cells.
Tsukagoshi M., Wada S., Hirono S., Yoshida S., Yada E., Sasada T., Shirabe K., Kuwano H, & Yamaue H. (2018). Identification of a novel HLA-A24-restricted cytotoxic T lymphocyte epitope peptide derived from mesothelin in pancreatic cancer. Oncotarget, 9(59), 31448-31458.
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2

Cell Lines for HLA-A Phenotyping

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T2 (HLA-A*02:01, lymphoblast), Jiyoye (HLA-A32, Burkitt's lymphoma), EB-3 (HLA-A3/Aw32, Burkitt's lymphoma), Cercopithecus aethiops-derived COS7 and A498 (HLA-A*02:01, kidney carcinoma) cells were purchased from the American Type Culture Collection (Rockville, MD). PSCCA0922 (HLA-A*02:06/A*31:01, a B cell line) was provided by the Health Science Research Resources Bank (Osaka, Japan). Caki-1 (HLA-A*24:02/A*23:01, renal clear cell carcinoma) cells were provided by the Cell Resource Center for Biomedical Research Institute of Development, Aging and Cancer at Tohoku University. The HIG2 expression in A498 and Caki-1 cells was confirmed by a Western blotting analysis [24] (link). T2, Jiyoye, EB-3 and PSCCA0922 cells were maintained in RPMI1640 (Invitrogen, Carlsbad, CA), A498 and Caki-1 cells were maintained in EMEM (Invitrogen) and COS7 cells were maintained in DMEM (Invitrogen). Each medium was supplemented with 10% fetal bovine serum (GEMINI Bio-Products, West Sacramento, CA) and 1% antibiotic solution (Sigma-Aldrich, ST. Louis, MO).
Yoshimura S., Tsunoda T., Osawa R., Harada M., Watanabe T., Hikichi T., Katsuda M., Miyazawa M., Tani M., Iwahashi M., Takeda K., Katagiri T., Nakamura Y, & Yamaue H. (2014). Identification of an HLA-A2-Restricted Epitope Peptide Derived from Hypoxia-Inducible Protein 2 (HIG2). PLoS ONE, 9(1), e85267.
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3

Cell Lines for Immunology Research

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TISI cells (HLA-A*24:02/-, lymphoblastoid cell) were purchased from the IHWG Cell and Gene Bank (Seattle, WA). T2 (HLA-A*02:01/-, lymphoblast), Jiyoye (HLA-A32, Burkitt’s lymphoma), and EB-3 (HLA-A3/Aw32, Burkitt’s lymphoma) cells were purchased from American Type Culture Collection (Manassas, VA). HEV0011 (HLA-A*02:06/-, B lymphocytes) cells were purchased from RIKEN Bioresource Research Center (Tsukuba, Japan). All cells were cultured in RPMI1640 media (GIBCO) supplemented with 10% fetal bovine serum (GIBCO) and 1% antibiotic solution (Wako).
Hikichi T., Sakamoto M., Harada M., Saito M., Yamane Y., Tokumura K, & Nakamura Y. (2022). Identification of cytotoxic T cells and their T cell receptor sequences targeting COVID-19 using MHC class I-binding peptides. Journal of Human Genetics, 67(7), 411-419.
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4

Generating HLA-expressing C1R Cells

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We purchased C1R (B lymphoblasts lacking endogenous human leukocyte antigen (HLA)-A and HLA-B expression), Jiyoye, and EB-3 cells from the American Type Culture Collection (Rockville, MD, USA) and cultured them in RPMI1640 supplemented with 10% FBS. We developed C1R cells stably expressing HLA-A*02:01 or HLA-A*24:02 (C1R-A0201 or C1R-A2402, respectively) in our previous study [4 (link)]. We generated C1R-A0206, C1R-A1101, C1R-A3101, and C1R-A3303 by the nucleofection of pCAGGS vectors encoding HLA-A*02:06, HLA-A*11:01, HLA-A*31:01, and HLA-A*33:03 cDNAs, respectively.
We used the following anti-human antibodies for cell surface staining by flow cytometry analyses: We purchased CD3-APC (cat# 300311), CD3-APC-Cy7 (clone HIT3a, cat# 300317), CD4-PE-Cy7 (clone RPA-T4, cat# 300511), CD8-FITC (cat# 560960), and CD8-PE-Cy7 (clone HIT8a, cat# 301012) from BioLegend (San Diego, CA, USA). We also obtained an anti-mouse TCRβ chain-APC antibody (clone H57-597, cat# 109212) from BioLegend (San Diego, CA, USA). We purchased peptide-HLA tetramers labeled with PE from MBL (Tokyo, Japan). We analyzed all flow cytometry data using FlowJo software (ver.10, BD Biosciences).
Tate T., Matsumoto S., Nemoto K., Leisegang M., Nagayama S., Obama K., Nakamura Y, & Kiyotani K. (2023). Identification of T Cell Receptors Targeting a Neoantigen Derived from Recurrently Mutated FGFR3. Cancers, 15(4), 1031.
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