S. oralis strain ATCC 9811 was obtained from the American Type Culture Collection (ATCC) and P. gingivalis strain ATCC 33277/DSM 20709 from the German Collection of Microorganisms and Cell Cultures (DSMZ). The strains were grown under anaerobic conditions (80% N2, 10% H2, 10% CO2; anaerobic workbench Concept 400-M, Ruskinn Technology Ltd., Pencoed, UK) at 37 °C for 24 h in 50 mL screw cap tube (Sarstedt, Nümbrecht, Germany) with Brain Heart Infusion Medium (BHI; Oxoid, Wesel, Germany) containing 10 µg/mL vitamin K (Roth, Karlsruhe, Germany; BHI/VitK) to obtain 24-h-old precultures for further processing.
Atcc 9811
Manufactured by American Type Culture Collection
ATCC 9811 is a laboratory incubator designed for maintaining and growing cell cultures. It provides a controlled environment for temperature, humidity, and carbon dioxide levels to support the growth and proliferation of various cell types.
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Lab products found in correlation
2 protocols using atcc 9811
1
Cultivation of Oral Pathogens
2
Cultivation of Oral Bacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
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S. oralis strain ATCC 9811 was purchased from the American Type Culture Collection (ATCC) and P. gingivalis strain ATCC 33277/DSM 20709 from the German Collection of Microorganisms and Cell Cultures (DSMZ). To obtain 24 h-old precultures for further processing, both bacterial strains were cultured anaerobically (80% N2, 10% H2, 10% CO2; anaerobic workbench Concept 400-M, Ruskinn Technology Ltd., Pencoed, UK) for 24 h at 37 °C in Brain Heart Infusion Medium (BHI; Oxoid, Wesel, Germany) supplemented with 10 µg/mL vitamin K (Roth, Karlsruhe, Germany; BHI/VitK).
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