A549 non small cell lung carcinoma male

Human A549 non-small cell lung carcinoma (male), HeLa cervix carcinoma and SKOV3 ovarian carcinoma were obtained from American Type Culture Collection (ATCC). The cells were authenticated by ATCC and used within six months after thawing. Human pancreatic duct epithelial cells (H6C7) were purchased from Kerafast. Human immortalized mammary fibroblasts (HMF3) were kindly provided by Michael J. O'Hare (Ludwig Institute for Cancer Research, London, UK).61 (link) Lenti-X™ 293T human embryonic kidney epithelial cells transformed with Adenovirus type 5 DNA were from Clontech (632180). A549-triple cells (here referred to as A549-RFP-STAT3 cells) were kindly provided by Dmitry Malkov (Sigma-Aldrich, St. Louis, Missouri, USA).34 (link) Human MCF7 mammary carcinoma cells stably transfected with inducible pTRE-p95ErbB2 or corresponding pTRE vector were established and cultured as described previously.13 (link),43 (link) The expression of p95ErbB2 was induced by washing off the tetracycline (1 µg/ml) three passages prior to the experiment. H6C7 cells were cultured in SFM. Other cells were cultured in DMEM medium supplemented with 10% heat-inactivated fetal calf serum and penicillin/streptomycin (complete medium), and for H6C7cells also with 2 mM glutamine. Cells were maintained at 37°C and 5% CO₂. All cell lines were found negative for mycoplasma using Venor^®GeM Classic PCR kit.