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Ab124827

Manufactured by Abcam
Sourced in United States

Ab124827 is a laboratory equipment product from Abcam. It serves the core function of [CORE FUNCTION]. No further details or interpretation about the intended use of this product are provided.

Automatically generated - may contain errors

2 protocols using ab124827

1

Autophagy and Oxidative Stress Analysis

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Cell lysis and western blot were performed as described previously (Huang M, Leukemia 2013). Antibodies used for Immunoblots: anti-LC3B ( #2775, Cell Signaling), anti-Keap1 (sc-365626, G-2, Santa Cruz Biotechnology), anti-Atg3 (sc-393660, A-3, Santa Cruz Biotechnology), anti-NQO1 (sc-393736, F-8, Santa Cruz Biotechnology), anti-GCLM (Ab124827, Abcam), anti-Bcl2 (sc-56015, 100/D5, Santa Cruz Biotechnology), anti- p62 (sc-25575, H-290, Santa Cruz Biotechnology), anti-actin (A5441, Sigma), and anti-Nrf2 (sc-13032, H-300, Santa Cruz Biotechnology), anti-Brd4 (A301-985A50, BETHYL Laboratories). Anti-CEBPβ (sc-7962, H-7, Santa Cruz Biotechnology).
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2

Western Blot Analysis of SDF-1 and CXCR4

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The proteins were extracted from heart samples using RIPA Lysis Buffer [Sangon Biotech (Shanghai), Co., Ltd.]. Western blotting was performed with anti-SDF-1 (ab25117, abcam), anti-CXCR4 (ab124827, abcam), and anti-GAPDH (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, United States) primary antibodies and with relevant secondary anti-bodies (Santa Cruz Biotechnology, Inc.) as described previously (Chen et al., 2015 (link)).
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