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2 protocols using anti prdm16 polyclonal antibody abd130

1

Adipogenic Differentiation Antibody Reagents

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Antibodies against AMPKα (#2532), phospho-AMPKα at Thr172 (#2535), pyruvate dehydrogenase (PDH) (#2784), cytochrome c (#4280), β-tubulin (#2146) and goat anti-rat antibody Alexa Fluor 488 (#4416) were purchased from Cell Signaling (Danvers, MA). Anti-PRDM16 polyclonal antibody (#ABD130) was purchased from Millipore (Billerica, MA). Anti-UCP1 polyclonal antibody (#sc28766) was bought from Santa Cruz Biotechnology (Dallas, TX). Goat anti-rabbit IRDye 800CW (#926-32211) and goat anti-rabbit IRDye 680RD (#926-68070) secondary antibodies for western blot were purchased from LI-COR (Lincoln, NE). Fluoro-Gel II with 40, 6-diamidino-2-phenylindole (DAPI) (#17985-50) was purchased from Electron Microscopy Sciences (Hatfield, PA). Insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine (IBMX), Triiodothyronine (T3), Oil-Red O, and compound C were purchased from Sigma (St. Louis, MO). Collagenase D and dispase II were purchased from Roche Diagnostics (Indianapolis, IN). DMEM/F12 and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY).
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2

Adipogenic Differentiation Antibody Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against AMPKα (#2532), phospho-AMPKα at Thr172 (#2535), pyruvate dehydrogenase (PDH) (#2784), cytochrome c (#4280), β-tubulin (#2146) and goat anti-rat antibody Alexa Fluor 488 (#4416) were purchased from Cell Signaling (Danvers, MA). Anti-PRDM16 polyclonal antibody (#ABD130) was purchased from Millipore (Billerica, MA). Anti-UCP1 polyclonal antibody (#sc28766) was bought from Santa Cruz Biotechnology (Dallas, TX). Goat anti-rabbit IRDye 800CW (#926-32211) and goat anti-rabbit IRDye 680RD (#926-68070) secondary antibodies for western blot were purchased from LI-COR (Lincoln, NE). Fluoro-Gel II with 40, 6-diamidino-2-phenylindole (DAPI) (#17985-50) was purchased from Electron Microscopy Sciences (Hatfield, PA). Insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine (IBMX), Triiodothyronine (T3), Oil-Red O, and compound C were purchased from Sigma (St. Louis, MO). Collagenase D and dispase II were purchased from Roche Diagnostics (Indianapolis, IN). DMEM/F12 and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY).
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