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Ml 297

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ML 297 is a laboratory instrument used for molecular biology research. It is designed to perform polymerase chain reaction (PCR) analysis. The core function of the ML 297 is to amplify specific DNA sequences through thermal cycling.

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Lab products found in correlation

Thapsigargin, by R&D Systems (4 mentions) Picrotoxin, by R&D Systems (4 mentions) Chelerythrine, by R&D Systems (4 mentions) Heparin, by R&D Systems (4 mentions) U73122, by R&D Systems (4 mentions) Ml 133, by R&D Systems (4 mentions) Gdp β s, by R&D Systems (3 mentions) U73343, by R&D Systems (3 mentions) Bisindolylmaleimide 2 bis 2, by R&D Systems (3 mentions) Dioctanoyl phosphatidylinositol 4 5 bisphosphate dic8 pip2, by Echelon Biosciences (3 mentions) Tertiapin q, by R&D Systems (3 mentions) Kynurenic acid, by R&D Systems (3 mentions) Sr49059, by R&D Systems (2 mentions) Tertiapin lq, by R&D Systems (2 mentions) Bis 2, by R&D Systems (1 mentions) Bapta, by R&D Systems (1 mentions) Capsazepine, by R&D Systems (1 mentions) Kb r7943, by R&D Systems (1 mentions) Capsaicin, by R&D Systems (1 mentions) Sch23390, by R&D Systems (1 mentions)

4 protocols using ml 297

1

Pharmacological Reagents for Neuronal Research

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The following chemicals were products of R&D Systems: AVP, TTX, kynurenic acid, picrotoxin, 6,7-dinitroquinoxaline-2,3-dione (DNQX), SR49059, GDP-β-S, U73122, U73343, heparin, thapsigargin, chelerythrine, bisindolylmaleimide II (Bis II), ML 133, ML 297, tertiapin-Q and tertiapin-LQ. Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP2) was purchased from Echelon Biosciences. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.
Hu B., Boyle C.A, & Lei S. (2021). Roles of PLCβ, PIP2 and GIRK Channels in Arginine Vasopressin-elicited Excitation of CA1 Pyramidal Neurons. Journal of cellular physiology, 237(1), 660-674.
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2

Neuronal Activity Modulation Protocols

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The following chemicals were products of R&D Systems: [Thr4,Gly7]-oxytocin (TGOT), kynurenic acid, picrotoxin, GDP-β-S, U73122, U73343, heparin, thapsigargin, BAPTA, chelerythrine, bisindolylmaleimide II (Bis II), phorbol 12-myristate 13-acetate (PMA), tetrodotoxin (TTX), ML 133, ML 297, tertiapin-Q and tertiapin-LQ. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals which are only soluble in dimethyl sulfoxide (DMSO), the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.
Hu B., Boyle C.A, & Lei S. (2020). Oxytocin Receptors Excite Lateral Nucleus of Central Amygdala by PLCβ and PKC-dependent Depression of Inwardly Rectifying K+ Channels. The Journal of physiology, 598(16), 3501-3520.
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3

Detailed Chemical Source Protocol

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The following chemicals were products of R&D Systems: senktide, TTX, picrotoxin, SB218795, ML133, glibenclamide, tertiapin-Q, ML297, M084, U73122, heparin, thapsigargin, chelerythrine and bisindolylmaleimide II (Bis II). Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP2) was purchased from Echelon Biosciences. The other chemicals were the products of Sigma Aldrich. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%.
Boyle C.A., Hu B., Quaintance K.L., Mastrud M.R, & Lei S. (2022). Ionic signaling mechanisms involved in neurokinin-3 receptor-mediated augmentation of fear-potentiated startle response in the basolateral amygdala. The Journal of physiology, 600(19), 4325-4345.
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4

Pharmacological Agents in Neuronal Signaling

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The following chemicals were products of R&D Systems: AVP, tetrodotoxin (TTX), kynurenic acid, picrotoxin, SR49059, TASP0390325, tolvaptan, GDP-β-S, U73122, U73343, heparin, thapsigargin, chelerythrine, bisindolylmaleimide II (Bis II), KB-R7943, capsazepine, AMG9810, AMG1629, capsaicin, ML 133, ML 297 and SCH23390. Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP2) was purchased from Echelon Biosciences. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.
Lei S., Hu B, & Rezagholizadeh N. (2021). Activation of V1a Vasopressin Receptors Excite Subicular Pyramidal Neurons by Activating TRPV1 and Depressing GIRK Channels. Neuropharmacology, 190, 108565.
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