Cd45 alexa 700 30 f11

Manufactured by BioLegend

CD45 Alexa 700 (30-F11) is a lab equipment product that can be used to label and identify cells expressing the CD45 antigen. It contains an anti-CD45 antibody conjugated to the Alexa Fluor 700 fluorescent dye.

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Lab products found in correlation

Ly6g pe cy7, by BioLegend (1 mentions) Cd163 apc, by BioLegend (1 mentions) F4 80 brilliant violet 785, by BioLegend (1 mentions) Cd4 pe cy7 gk1.5, by BioLegend (1 mentions) Siglecf pe, by BD (1 mentions) Ly6g bv421, by BioLegend (1 mentions) F4 80 apc bm8, by BioLegend (1 mentions) Anti cd16 32, by BioLegend (1 mentions) Cd11b bv510 m1 70, by BioLegend (1 mentions) Cd11c fitc n418, by BioLegend (1 mentions)

Spelling variants of this product

CD45 (30-F11, (72 citations) Alexa Fluor 700-labelled anti-CD45 antibody (clone 30-F11, (2 citations)

3 protocols using cd45 alexa 700 30 f11

Multicolor Flow Cytometry Panel

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CD45 Alexa 700 (30-F11, Biolegend), CD11b PerCP/Cyanine5.5 (M1/70, Biolegend), CD11c Brilliant Violet 510 (N418, Biolegend), F4/80 Brilliant Violet 785 (BM8, Biolegend), Ly-6G PE/Cy7 (1A8, Biolegend), Ly-6C Brilliant Violet 605 (HK1.4, Biolegend), CD80 Brilliant Violet 421 (16-10A1, Biolegend), CD163 APC (S15049I, Biolegend), and CD285 PE (TLR-5) (ACT5, BD Biosciences) (Supplementary Table 11).

Gonzalez C., Williamson S., Gammon S.T., Glazer S., Rhee J.H, & Piwnica-Worms D. (2023). TLR5 agonists enhance anti-tumor immunity and overcome resistance to immune checkpoint therapy. Communications Biology, 6, 31.

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Multiparameter Immune Cell Profiling

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CD45 Alexa 700 (30-F11, Biolegend), CD19 PerCP/Cy5.5 (1D3/CD19, Biolegend), CD3ε Brilliant Violet 510 (17A2, Biolegend), CD4 PE/Cy7 (GK1.5, Biolegend), CD49b BV421 (DX5, Biolegend), CD8a Brilliant Brilliant Violet 711 (53–6.7, Biolegend), FoxP3 Alexa 647 (MF-14, Biolegend), and CD285 PE (TLR-5) (ACT5, BD Biosciences) (Supplementary Table 11).

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Quantifying Bacterial Loads and Immune Cells

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Bacterial loads were determined in BALF and blood. Serial dilutions of samples were plated on blood agar and colony forming units (CFU) were counted. To test for cell recruitment, cells were enriched by spinning the BALF (400 g 10 min RT), incubated with blocking antibody (anti-CD16/32, Biolegend) and stained using CD11c-FITC (N418, Biolegend), SiglecF-PE (E50-2440, BD bioscience), Ly6C-PerCp (HK1.4, Biolegend), F4/80-APC (BM8, Biolegend), CD45-Alexa700 (30-F11, Biolegend), Ly6G-BV421 (1A8, Biolegend) and CD11b-BV510 (M1/70, Biolegend).

Kostadinova E., Chaput C., Gutbier B., Lippmann J., Sander L.E., Mitchell T.J., Suttorp N., Witzenrath M, & Opitz B. (2016). NLRP3 protects alveolar barrier integrity by an inflammasome-independent increase of epithelial cell adherence. Scientific Reports, 6, 30943.

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