Spleen cells were excised from mice and single-cell suspensions of splenocytes were prepared in RPMI 1640 by teasing the organ through a sterile nylon mesh. Naive CD4+ T cells were purified using an EasySep mouse naive CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada) and cultured with plate-bound anti-CD3 (5 µg/ml; Bio X Cell), anti-CD28 (5 µg/ml; Bio X Cell), and cytokines used alone, including IL-12 (R&D Systems), IFN-γ (R&D Systems), IL-1 (R&D Systems), IL-4 (R&D Systems), IL-6 (Cell Signaling Technology), IL-23 (eBioscience), and TGF-β (Invitrogen), or in combination with Abs (Bio X Cell) for Th17-promoting (2.5 ng/ml TGF-β, 20 ng/ml IL-6, 10 µg/ml anti–IFN-γ, 10 µg/ml anti–IL-4, 10 µg/ml anti–IL-2), Th1-promoting (10 ng/ml IL-12, 5 µg/ml anti–IL-4), Th2-promoting (10 ng/ml IL-4, 5 µg/ml anti–IFN-γ), or regulatory T cell (Treg)–promoting (5 ng/ml TGF-β, 5 µg/ml anti–IFN-γ, 5 µg/ml anti–IL-4) conditions. In some experiments, naive CD4+ T cells were cultured in Th17-promoting conditions and IL-21 (20 ng/ml; eBioscience) or IL-23 (50 ng/ml) was added to cultures after 24 h. Cells were harvested after 48 h for real-time PCR analysis and after 72 h for cytokine expression by flow cytometry as previously described (23 (link)).
Ifn γ
Manufactured by BioXCell
Sourced in Canada
IFN-γ is a recombinant protein that functions as an interferon gamma. It is a cytokine that plays a crucial role in the immune response.
Automatically generated - may contain errors
Lab products found in correlation
Tgf β, by Thermo Fisher Scientific (1 mentions)
Anti cd3, by BioXCell (1 mentions)
Anti ifn γ, by BioXCell (1 mentions)
Il 21, by Thermo Fisher Scientific (1 mentions)
Anti cd28, by BioXCell (1 mentions)
Il 12, by BioXCell (1 mentions)
Tgf β, by BioXCell (1 mentions)
Interleukin 6 (il 6), by BioXCell (1 mentions)
Interleukin 4 (il 4), by BioXCell (1 mentions)
Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)
Il 23, by Thermo Fisher Scientific (1 mentions)
Easysep mouse naive cd4 t cell isolation kit, by STEMCELL (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Interleukin 4 (il 4), by R&D Systems (1 mentions)
Il 12, by R&D Systems (1 mentions)
Ctla4 ig, by Bristol-Myers Squibb (1 mentions)
Interleukin 7 (il 7), by BioXCell (1 mentions)
Anti ctla4, by BioXCell (1 mentions)
Cd40l, by BioXCell (1 mentions)
Mouse igg2a, by BioXCell (1 mentions)
3 protocols using ifn γ
1
Differentiation of Naive CD4+ T Cells
2
Modulating Immune Responses with mAbs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were injected intraperitoneally (i.p) one day before infection with either 100 μg of mAb to NK1.1 (PK136, BioXcell) or 200 μg of mAb to IFN-γ (BioXcell) or its isotype control and the procedure was repeated twice a week until completion of the experiment and sacrifice of the mice. 2-DG (1g/kg, Sigma) was injected every other day starting from the day of infection and until completion of the experiment. For glucose supplementation, mice were treated with drinking water containing 30% (w/v) glucose (started 1 week before infection until sacrifice). To inducibly delete mTOR in Id2-expressing cells, Id2CreERT2 Rosa26tdRFP mice with or without mTOR flox/flox alleles (Id2iΔmTOR) received oral gavage of 5mg Tamoxifen in corn oil four times over the course of 10 days. One week later, mice subsequently received four doses of 100ng rIL-12 and rIL-18 intranasally over a 10-day period, following transient anesthesia with inhaled isoflurane, and were culled to harvest tissues one day after the final dose.
3
Antibody Modulation of Immune Checkpoints
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