NK-92MI cells were obtained from Procell Life Science&Technology Co, Ltd. They were cultures in αMEM(Gibco, Cat#41061029) medium supported with 12.5% heat-inactivated FBS(Hyclone, Cat#SH30088.03HI), 12.5% heat-inactivated horse serum (Gibco, Cat#26050088), 0.2 mM inositol (Solarbio, Cat#I8050), 0.1 mM 2-mercaptoethanol (Solarbio, Cat#M8210), and 0.02 mM folic acids (Solarbio, Cat#P1400). All media were supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin (Solarbio, Cat#P1400). Cells were cultivated at 37 °C in a humidified atmosphere of 5% CO2 in the air.
Inositol
Manufactured by Solarbio
Inositol is a naturally occurring sugar alcohol that plays a role in various cellular processes. It is commonly used in laboratory settings for cell culture and biochemical applications. Inositol serves as a precursor for important signaling molecules and can be involved in the regulation of cellular activities. This product is available in different grades and purities to meet the requirements of diverse research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 mercaptoethanol, by Solarbio (2 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Nk 92mi cells, by Procell (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Horse serum, by Sartorius (1 mentions)
Nk 92 mi cell line, by American Type Culture Collection (1 mentions)
Recombinant human interleukin 2, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Folic acid, by Merck Group (1 mentions)
Sucrose, by Solarbio (1 mentions)
Agar powder, by Solarbio (1 mentions)
3 protocols using inositol
1
Cell Culture of NK-92MI Cells
2
Culturing NKTCL and NK-92 MI Cell Lines
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Three NKTCL cell lines—SNT-8, SNK-10, and SNT-16 (kind gifts from Dr. Norio Shimizu at Tokyo Medical and Dental University)—were cultured in RPMI-1640 medium (Hyclone) supplemented with 10% human plasma (Gemini) and 700 U/mL recombinant human interleukin-2 (PeproTech). The NK-92 MI cell line was purchased from ATCC (CRL-2408) and cultured in Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides (Gibco) but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate (Life Technologies) supplemented with 12.5% fetal bovine serum (Biological Industries), 12.5% horse serum (Biological Industries), 0.2 mM inositol (Solarbio), 0.1 mM 2-mercaptoethanol (Solarbio) and 0.02 mM folic acid (Sigma Aldrich). All cells were cultivated at 37°C in a humidified atmosphere of 5% CO2 in the air.
3
Plant Growth Regulator Experiment
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The main reagents used in this experiment were 6-BA (6-benzylamino purine), NAA (naphthalene acetic acid), CuSO 4 , AgCl, sucrose, MS basic medium, inositol and agar powder, which were purchased from Beijing Solarbio Science & Technology Co., Ltd.
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