Blood glucose and beta-hydroxybutyrate (BHB) were monitored daily using a Precision Xtra Blood Glucose and Ketone Monitoring System (Abbott Laboratories, IL, USA). An 8 mL blood sample was either drawn from the sampling catheter (days 0 to 5) or collected from the coccygeal vein (days 6 to 12) and blood glucose and BHB were immediately measured.
Precision xtra blood glucose and ketone monitoring system
Manufactured by Abbott
Sourced in United States
The Precision Xtra Blood Glucose and Ketone Monitoring System is a device used to measure blood glucose and ketone levels. It provides accurate and reliable readings to assist in the management of diabetes and ketosis.
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Lab products found in correlation
6 protocols using precision xtra blood glucose and ketone monitoring system
1
Daily Blood Glucose and BHB Monitoring
2
Ketone Monitoring in Traumatic Brain Injury
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Ketone bodies in the blood were measured using the Precision Xtra Blood Glucose and Ketone Monitoring System (Abbott, Columbus, OH)60 (link). Mice were anesthetized with isoflurane and tails were cut approximately 0.2 mm from the end, a drop of blood was squeezed directly into a testing strip attached to the measuring instrument. Group sizes were as follows: control (n = 8), mTBI (n = 6), KD (n = 6), and mTBI + KD (n = 5).
3
Dietary Strategies for Sustained Ketosis in T2D
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The Virta Clinic includes individualized nutritional recommendations to sustain nutritional ketosis by titrating carbohydrate and protein intake to the patient’s individual tolerance [27 ]. With the insulin resistance characteristic of type 2 diabetes, subjects typically require total dietary carbohydrates to be restricted to <30 g·day−1. Daily protein intake was targeted to a level of 1.5 g·kg−1 of reference (ie, medium-frame “ideal”) body weight and participants were coached to incorporate dietary fats to satiety. Other aspects of the diet were individually prescribed to ensure safety, effectiveness, and satisfaction, including consumption of 3-5 servings of nonstarchy vegetables and adequate mineral and fluid intake for the ketogenic state. BOHB was monitored routinely via finger-stick blood monitoring using a handheld device (Abbott Precision Xtra Blood Glucose and Ketone Monitoring System, Alameda, CA, USA) and participants were encouraged to obtain BOHB readings ≥0.5 mmol·L−1.
4
Glucose and Lipid Metabolism Assessment
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At weeks 0, 4, 8, and 11, blood glucose readings were measured after overnight fasting (16 h). Blood was collected from the lateral tail vein and immediately assessed for glucose levels using the Precision Xtra Blood Glucose and Ketone Monitoring System (Abbott Diabetes Care, Alameda, CA, USA).
At week 11, glucose tolerance testing (GTT) was performed. First, baseline fasting glucose levels were taken. Mice were then administered a glucose bolus (2 g/kg body weight) via IP injection. Blood glucose levels were recorded from lateral tail vein 15, 30, 60, and 120 min after the glucose bolus. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were measured enzymatically at the conclusion of the experimental period. Commercially available kits for both cholesterol (Total Cholesterol Colorimetric Assay kit, #K603, BioVision, Milpitas, CA, USA) and triglycerides (LabAssay Triglycerides, #290-63701, Wako Chemicals, Richmond, VA, USA) were used following the manufacturers’ protocols.
At week 11, glucose tolerance testing (GTT) was performed. First, baseline fasting glucose levels were taken. Mice were then administered a glucose bolus (2 g/kg body weight) via IP injection. Blood glucose levels were recorded from lateral tail vein 15, 30, 60, and 120 min after the glucose bolus. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were measured enzymatically at the conclusion of the experimental period. Commercially available kits for both cholesterol (Total Cholesterol Colorimetric Assay kit, #K603, BioVision, Milpitas, CA, USA) and triglycerides (LabAssay Triglycerides, #290-63701, Wako Chemicals, Richmond, VA, USA) were used following the manufacturers’ protocols.
5
Fitbit-based Tracking of Physical Activity, Energy Expenditure, and Sleep Quality
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Physical activity, daily energy expenditure, and sleep quality were measured daily with a Fitbit Versa 2 activity tracker and its software (www.fitbit.com ) accessible on the cell phone daily and from the Fitbit weekly email reports. The device tracked daily number of steps and distance travelled. Besides daily walking, the subject engaged in two hours per week of Ashtanga yoga and resistance exercise using weight machines in the university gym. RMR was measured with the Tanita bioimpedance apparatus on days 185, 257, 345, 412, 450. 505, and 574.
Sleep quality was assessed from daily records of minutes of sleep collected by Versa-2 fitbit activity monitor. The variables tallied in nightly minutes were total, light (stages N1 and N2), deep (stage N3), and rapid-eye-movement (REM) sleep, and of awake periods during the sleep period, as well as number of brief awakenings.
Beta-hydroxybutyrate (BHB) was measured in lanced blood samples applied to ketone strips with the Precision Xtra blood glucose and ketone monitoring system (Abbott Diabetes Care Inc, Alameda CA). Measurements were done on days 251 through 265 between 07:00 and 07:30 h after the 38-h fast day and on the three post-fast eating days, D1, D2, and D3. A single measurement was done on day 266 after 60 hours of UF resulting from two consecutive days of fast.
6
Metabolic Characterization of Mouse Model
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Blood glucose readings were measured after overnight fasting (16 h) every four weeks beginning at week 0 of the 12-week treatment period. Blood was collected from the lateral tail vein and immediately assessed for glucose levels using the Precision Xtra Blood Glucose and Ketone Monitoring System (Abbott Diabetes Care).
Glucose tolerance testing (GTT) was performed at week 11. Fasting, baseline glucose readings were taken after which mice were administered a glucose bolus (2 g/kg body weight) via oral gavage. Blood glucose levels were recorded 15, 30, 60, and 120 min after the glucose bolus was given. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were enzymatically determined at the conclusion of the experiment using commercially available kits (LabAssay Triglycerides #290-63701, Wako Chemicals; Total Cholesterol Colorimetric Assay kit, #K603, BioVision). All samples were run in duplicate according to manufacturer’s instructions.
Glucose tolerance testing (GTT) was performed at week 11. Fasting, baseline glucose readings were taken after which mice were administered a glucose bolus (2 g/kg body weight) via oral gavage. Blood glucose levels were recorded 15, 30, 60, and 120 min after the glucose bolus was given. Area under the curve (AUC) was calculated.
Plasma cholesterol and triglyceride levels were enzymatically determined at the conclusion of the experiment using commercially available kits (LabAssay Triglycerides #290-63701, Wako Chemicals; Total Cholesterol Colorimetric Assay kit, #K603, BioVision). All samples were run in duplicate according to manufacturer’s instructions.
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