Anti rabbit igg irdye 680

Manufactured by LI COR

Sourced in United States

The Anti-rabbit IgG IRDye 680 is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with the near-infrared fluorescent dye IRDye 680 for detection using infrared imaging systems.

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3 protocols using anti rabbit igg irdye 680

Immunostaining and Western Blot Protocols

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Primary antibodies used were rabbit anti-Ezh2 (Cell Signaling Technology), rabbit anti-H3K27me3 (Cell Signaling Technology), rabbit anti-α Tubulin (Abcam), rabbit anti-H3 (Cell Signaling Technology), mouse anti-Reelin (Millipore), rabbit anti-GRASP65 (Abcam), rabbit anti-Cux1 (Santa Cruze), rabbit normal IgG (Applygen). Second antibodies included anti-rabbit IgG IRDye 680 and anti-mouse IgG IRDye 800 (LICOR Bioscience) for western blot and Alexa 488 anti-rabbit IgG, alexa 555 anti-rabbit IgG (Invitrogen) for immunostaining.

Zhao L., Li J., Ma Y., Wang J., Pan W., Gao K., Zhang Z., Lu T., Ruan Y., Yue W., Zhao S., Wang L, & Zhang D. (2015). Ezh2 is involved in radial neuronal migration through regulating Reelin expression in cerebral cortex. Scientific Reports, 5, 15484.

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Immunoblotting of PTEN and GFP Proteins

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Yeast extracts, SDS-PAGE and immunoblotting were performed as previously described [40 (link)].Primary antibodies used were anti-PTEN (C-Term)-clone Y184 (Cat. #04-409 Millipore^®, Burlington, MA, USA), anti-GFP-JL8 (Cat. #632380, Living Colors^®, Takara, Mountain View, CA, USA) and anti-G6PDH (Sigma, St. Louis, MO, USA; 1:50,000 dilution). Secondary antibodies were anti-mouse IgG-Alexa Fluor^® 680, anti-rabbit IgG-IRDye^® 800CW and anti-rabbit IgG-IRDye^® 680; all from LI-COR (Lincoln, NE, USA) at 1:5,000 dilution.

Fernández-Acero T., Bertalmio E., Luna S., Mingo J., Bravo-Plaza I., Rodríguez-Escudero I., Molina M., Pulido R, & Cid V.J. (2019). Expression of Human PTEN-L in a Yeast Heterologous Model Unveils Specific N-Terminal Motifs Controlling PTEN-L Subcellular Localization and Function. Cells, 8(12), 1512.

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Protein Expression Detection Methods

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Primary antibodies used were rabbit anti-Gas7 (Sigma), rabbit anti-Cux1 (Santa Cruze), rabbit anti-GFP (Invitrogen), mouse anti-actin-mCherry (Sigma), rabbit anti-GAPDH (Cell Signaling Technology) and mouse anti-MYC (Santa Cruze) and alexa-546 phalloidin (Invitrogen).
Secondary antibodies included anti-rabbit IgG IRDye 680 and anti-mouse IgG IRDye 800 (LICOR Bioscience) for western blot and Alexa 488 anti-rabbit IgG, alexa 555 anti-rabbit IgG (Invitrogen) for immunostaining.

Zhang Z., Zheng F., You Y., Ma Y., Lu T., Yue W, & Zhang D. (2016). Growth arrest specific gene 7 is associated with schizophrenia and regulates neuronal migration and morphogenesis. Molecular Brain, 9, 54.

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