Liquid Chromatography (LC) was conducted using the Dionex UltiMate 3000 Ultra High-performance Liquid Chromatography system (Thermo Fisher Scientific, Massachusetts, USA) equipped with a Thermo Syncronis C18 column (2.1 mm × 100 mm × 1.7 µm) (Thermo Fisher Scientific). During the analysis, the temperature was retained at 55 °C at a flow rate of 450 µL/min. Solvent A (water mixed with 0.1% formic acid) and solvent B (acetonitrile mixed with 0.1% of formic acid) were used as the mobile phases. Beginning at 0.5% of solvent B, the gradient elution program was set for 1 min, followed by 15 min from 0.5% to 99.5% of solvent B before being maintained for 4 min, and returned to 0.5% of solvent B in 2 min. The injection volume was set at 2 µL.
Dionex ultimate 3000 ultra high performance liquid chromatography system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex UltiMate 3000 is an ultra high-performance liquid chromatography (UHPLC) system designed for high-resolution separation and analysis of complex samples. It features advanced technology for precise and sensitive detection of analytes.
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Lab products found in correlation
2 protocols using dionex ultimate 3000 ultra high performance liquid chromatography system
1
High-Performance Liquid Chromatography Analysis
2
Quantification of Lumefantrine and Metabolite
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Drug concentrations were determined using an liquid chromatography-mass spectrometry/mass spectrometry based assay, validated according to US Food and Drug Administration guidelines. In brief, plasma sample preparation was performed by protein precipitation using a Hybrid Solid Phase Extraction-Precipitation 96-wellplate plate (Supelco) and a Freedom EVO liquid handler system (Tecan). Internal standards were used to compensate for recovery and matrix effects. The extracted drugs were separated using a Dionex Ultimate 3000 ultra high performance liquid chromatography system (Thermo Fisher) equipped with a Zorbax SB-CN column (Agilent). An API500 0 triple-quadrupole mass spectrometer and Analyst 1.6.3 software (both ABSciex) were used for drug detection and quantification. The lower limit of quantification was 9.71 ng/mL for lumefantrine and 1.01 ng/mL for desbutyl-lumefantrine. Three replicates of quality control samples at low, middle, and high concentrations were included in the analysis to ensure precision and accuracy. The total coefficient of variation of all quality control samples were <8% during drug quantification of clinical samples.
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