Log In Sign up
The largest database of trusted experimental protocols

Virtual slide system vs110

Manufactured by Olympus
Visit Supplier
Sourced in Japan

The Virtual Slide System VS110 is a digital microscopy solution that digitizes glass slides into high-resolution virtual slides. The system captures images of the entire slide and compiles them into a single digital file, enabling users to view and navigate the slide content on a computer screen.

Automatically generated - may contain errors

Lab products found in correlation

Infinity alt gpt, by Thermo Fisher Scientific (1 mentions) Benchmark, by Roche (1 mentions) Biotin conjugated goat anti rabbit igg, by Agilent Technologies (1 mentions) Iview dab detection kit, by Roche (1 mentions) Cell conditioning solution cc1, by Roche (1 mentions) Axio imager a1, by Zeiss (1 mentions) Image pro, by Media Cybernetics (1 mentions) Polyclonal rabbit antibodies, by Abcam (1 mentions)

6 protocols using virtual slide system vs110

1

Comprehensive Liver Histopathology Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of liver histopathology by light microscopy, formalin-fixed paraffin-embedded liver sections were cut at 5 μm and stained with hematoxylin and eosin (H&E), sirius red, or immunohistochemically for cytokeratin-19 (CK-19) antigen by the Michigan State University Investigative Histopathology Laboratory as described previously (18 (link),31 (link)). Hepatocellular necrosis was quantified in a masked fashion by examining 5 representative 40X H&E-stained liver sections from each animal and expressed as percent of total tissue area. For quantification of sirius red (collagen deposits) and CK-19 staining (BDECs), images of stained liver sections were captured using a Virtual Slide System VS110 (Olympus, Hicksville, NY) with a 20X objective. The area of positive sirius red and CK-19 staining in at least 200 images per tissue was determined in an automated and unbiased fashion using a batch macro and the color de-convolution tool in ImageJ. Serum activity of alanine aminotransferase (ALT) was determined using commercial reagents (Infinity ALT/GPT Thermo Fisher, Waltham, MA).
Joshi N., Ray J.L., Kopec A.K, & Luyendyk J.P. (2016). Dose-Dependent Effects of Alpha-Naphthylisothiocyanate Disconnect Biliary Fibrosis from Hepatocellular Necrosis. Journal of biochemical and molecular toxicology, 31(1), 1-7.
+ Open protocol
+ Expand
2

Assessing DNA Damage in Tumor Microenvironment

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
CMS5a1 and CMS5a1γRDN cells were inoculated in the left flank and the right flank, respectively, of the same RAG−/− mouse. Eighteen days after tumour inoculation, the mice were treated with CD8+ DL cells of CMS5a1-bearing WT mice treated with anti-CD137 mAb on the same day and 7 days after tumour inoculation. Tumour masses were harvested on day 20 (2 days after ACT) from ACT-treated mice and control ACT-non-treated mice, fixed in 10% formalin, and then embedded in paraffin. Following hematoxylin/eosin (HE) staining of paraffin sections65 (link), to detect of phospho-Histone H2A.X (Ser139), paraffin sections were incubated with rabbit anti-phospho-Histone H2A.X (Ser139)(γH2A.X) mAb (clone 20E3) (Cell Signaling Technology, Denver, MA) and biotin-conjugated goat anti-rabbit IgG (Dako, Carpenteria, CA) in an automated immunostainer (BenchMark; Ventana Medical Systems, Tucson, AZ) by using an iVIEW DAB Detection Kit (Open Secondary; Ventana) and a Cell Conditioning Solution (CC1; Ventana). Finally, sections were counter-stained with hematoxylin, and were scanned in a Virtual Slide System (VS110; Olympus, Tokyo, Japan). The whole area of tumour margin was examined in each specimen, and the numbers of positively nucleus, % of intact area and % of cell death area were calculated by image analysis software, Tissue Studio v.2.3 (Definiens AG, Munich, Germany).
Takeda K., Nakayama M., Hayakawa Y., Kojima Y., Ikeda H., Imai N., Ogasawara K., Okumura K., Thomas D.M, & Smyth M.J. (2017). IFN-γ is required for cytotoxic T cell-dependent cancer genome immunoediting. Nature Communications, 8, 14607.
+ Open protocol
+ Expand
3

Histopathological Analysis of Liver Samples

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of liver histopathology by light microscopy, formalin-fixed liver sections were cut at 5 microns and stained with hematoxylin and eosin (H&E), sirius red, and cytokeratin-19 (CK-19) antigen by the Michigan State University Investigative Histopathology Laboratory as described previously (Joshi et al. 2015 (link); Joshi et al. 2016 (link)). At least 2 sections of liver from the left lateral lobe of each animal were qualitatively and quantitatively evaluated in their entirety to calculate area of necrosis. Quantitative assessment of necrotic area in H&E-stained sections were performed in a masked fashion. For quantification of sirius red (collagen deposits) and CK-19 staining (bile duct epithelial cells), images of stained liver sections were captured using a Virtual Slide System VS110 (Olympus, Hicksville, NY) with a 20× objective. The area of positive sirius red and CK-19 staining in at least 200 images per tissue was determined in an automated and unbiased fashion using a batch macro and the color de-convolution tool in ImageJ. Serum activity of alanine aminotransferase (ALT) was determined using a commercial reagent (Thermo Scientific, Waltham, MA). Plasma serotonin levels were determined using a commercial enzyme-linked immunosorbent assay kit (Eagle Biosciences, Nashua, NH).
Joshi N., Kopec A.K., Ray J.L, & Luyendyk J.P. (2016). Inhibition of PAR-4 and P2Y12 receptor-mediated platelet activation produces distinct hepatic pathologies in experimental xenobiotic-induced cholestatic liver disease. Toxicology, 365, 9-16.
+ Open protocol
+ Expand
4

Quantifying Platelet Accumulation in Liver Lesions

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
VWF and platelets were detected by immunofluorescent labeling of frozen liver sections, as described previously. (Groeneveld et al. 2020 (link)) Slides were scanned using a Virtual Slide System VS110 (Olympus) with a 20× objective. The area of positive VWF and platelet labeling was quantified using a batch macro and color de-convolution tool in Fiji (ImageJ) as previously described. (Groeneveld et al. 2020 (link)) For quantification of platelet accumulation within necrotic lesions after acute ANIT challenge, the area occupied by focal hepatocellular necrosis in each image was manually quantified by rough outline of areas in which the loss of hepatocellular DAPI was evident using the selection tool in Fiji (ImageJ). Platelet staining within each area was determined and CD41 staining per necrotic lesion calculated using the threshold and particle analyzing tool in Fiji (ImageJ).
Poole L.G., Fournier A.K., Cline-Fedewa H.M., Kopec A.K., Luyendyk J.P, & Groeneveld D.J. (2021). Von Willebrand factor exerts hepatoprotective effects in acute but not chronic cholestatic liver injury in mice. Toxicology, 463, 152968.
+ Open protocol
+ Expand
5

Quantifying Muscle Fiber Characteristics

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Virtual Slide System VS110 (Olympus, Tokyo, Japan) was used to scan slides from one section of each muscle stained with PSR. Image Pro (Media Cybernetics, version 6.0) was used to analyze the digital slides to quantify collagen deposition. For CSA quantification, images were captured using a coupled digital camera (Axio Imager A1, Carl Zeiss; Jena, Germany) and analyzed using NIH ImageJ software (Image Processing and Analysis in Java; ImageJ 64-bit). An average of 1468 ± 189 fibers from each muscle and each group were analyzed for CSA quantification, and all digitized sections were used for CC.
To analyze the frequency of the fiber-size distribution, the fibers were divided into different quartiles and categorized according to their size. In the gastrocnemius muscle, the fibers measuring less than 792 μm2 and 977 μm2, for CONT + SED and CONT + EX, respectively, were categorized as small (quartile 1–Q1), and those measuring more than 1616 μm2/1964 μm2, for CONT + SED and CONT + EX, respectively, were categorized as large (quartile 3–Q3). The same procedure was used for the soleus muscle (CONT + SED–Q1 < 950 μm2; Q3 > 1589 μm2 and CONT + EX–Q1 < 1030 μm2; Q3 > 1751).
Figueira A.C., Pereira A., Leitão L., Ferreira R., Oliveira P.A, & Duarte J.A. (2023). Effects of Moderate Exercise Training on Cancer-Induced Muscle Wasting. Healthcare, 11(19), 2652.
+ Open protocol
+ Expand
6

Quantitative Histopathological Analysis of Liver

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed, paraffin-embedded livers were cut at 5 microns and stained with picrosirius red, and immunohistochemically for cytokeratin-19 (CK-19), CD3 (T-cells), and CD45R (B-cells) by the Investigative Histopathology Laboratory at Michigan State University as described previously (Joshi et al. 2015 (link); Joshi et al. 2016a (link)). Primary antibodies utilized were polyclonal rabbit antibodies (Abcam, Cambridge, MA) and detected by HRP-conjugated polymer detection systems. Images comprising the entire left lateral lobe (>500 images) were captured using a Virtual Slide System VS110 (Olympus, Hicksville, NY) with a 20× objective. The area of positive sirius red and CK-19 staining was determined in an automated and unbiased fashion using a batch macro and the color de-convolution tool in ImageJ. Positive area for each stain was expressed individually and as a ratio of sirius red/CK-19. Similarly, the number of CD3 and CD45R-positive cells per image was determined using a batch macro, the color de-convolution tool, and particle analysis function of ImageJ.
Joshi N., Kopec A.K., Cline-Fedewa H, & Luyendyk J.P. (2016). Lymphocytes contribute to biliary injury and fibrosis in experimental xenobiotic-induced cholestasis. Toxicology, 377, 73-80.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!