For immunoblot, protein samples were resolved by SDS-PAGE, transferred to PVDF membranes (Millipore), and incubated with primary antibodies: anti-Ccnd1 (monoclonal DCS-6, BD biosciences, 1:500), anti-Gabra4 (polyclonal, AB5459, Merck, 1:1000, and polyclonal 12979-1-AP, Proteintech, 1:500), anti-Flag (polyclonal, 11508721, Invitrogen, 1:1000), anti-HA (rat monoclonal 3F10, Roche #11867431001, 1:5000), anti-TFR (monoclonal H68.4, Invitrogen #13-6800, 1:1,000) and anti-GST (goat polyclonal, Amersham #27-4577, 1:2000). Appropriate peroxidase-linked secondary antibodies (GE Healthcare UK Ltd) were detected using the chemiluminescent HRP substrate Immobilon Western (Millipore). Chemiluminescence was recorded with a ChemiDoc-MP imaging system (BioRad).
Anti gabra4
Manufactured by Merck Group
Anti-Gabra4 is a laboratory reagent used in research applications. It is an antibody that targets the GABRA4 protein, which is involved in the function of the GABA-A receptor. The core function of Anti-Gabra4 is to facilitate the detection and study of the GABRA4 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon western, by Merck Group (2 mentions)
Anti ccnd1, by BD (2 mentions)
Dcs 6, by BD (2 mentions)
Appropriate peroxidase linked secondary antibodies, by Merck Group (2 mentions)
Anti flag, by Thermo Fisher Scientific (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Chemidoc mp imaging system, by Bio-Rad (2 mentions)
2 protocols using anti gabra4
1
Immunoblotting Protein Detection Protocol
2
Immunoblotting Protein Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For immunoblot, protein samples were resolved by SDS-PAGE, transferred to PVDF membranes (Millipore), and incubated with primary antibodies: anti-Ccnd1 (monoclonal DCS-6, BD biosciences, 1:500), anti-Gabra4 (polyclonal, AB5459, Merck, 1:1000, and polyclonal 12979-1-AP, Proteintech, 1:500), anti-Flag (polyclonal, 11508721, Invitrogen, 1:1000), anti-HA (rat monoclonal 3F10, Roche #11867431001, 1:5000), anti-TFR (monoclonal H68.4, Invitrogen #13-6800, 1:1,000) and anti-GST (goat polyclonal, Amersham #27-4577, 1:2000). Appropriate peroxidase-linked secondary antibodies (GE Healthcare UK Ltd) were detected using the chemiluminescent HRP substrate Immobilon Western (Millipore). Chemiluminescence was recorded with a ChemiDoc-MP imaging system (BioRad).
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