HIV-1 testing was performed at each study visit using two rapid antibody test kits (Determine, Abbott Laboratories; Unigold, Trinity Biotech) in parallel. Discordant rapid HIV-1 test results were resolved using an enzyme-linked immunosorbent assay (ELISA) (Genetic System HIV-1/2 plus O EIA, Bio-Rad Laboratories). All HIV-1 negative samples were tested for p24 antigen (Vironostika HIV-1 p24 ELISA, Biomérieux) through 2015, or for HIV-1 RNA (Xpert® HIV-1 Qual, Cepheid) since 2016. Pre- and post-seroconversion samples were tested for HIV-1 RNA level (Amplicor Monitor 1.5, Roche). Gonococcal infection among participants who reported urethral or rectal symptoms was defined as the detection of gram-negative, intracellular diplococci consistent with Neisseria gonorrhoeae in urethral or rectal secretions.[7 (link)] Prevalent syphilis infection was diagnosed using a positive rapid plasma reagin (RPR, tested annually) titre confirmed by Treponema pallidum hemagglutination assay (TPHA). Incident syphilis was defined as a 4-fold increase in RPR titre confirmed by TPHA.[7 (link)]
Genetic system hiv 1 2 plus o eia
Manufactured by Bio-Rad
The Genetic System HIV-1/2 plus O EIA is a laboratory equipment used for the detection of antibodies to Human Immunodeficiency Virus Types 1 and 2, including the O (outlier) group, in human serum or plasma. It is an enzyme immunoassay (EIA) that provides a qualitative result to determine the presence or absence of the target antibodies.
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Lab products found in correlation
2 protocols using genetic system hiv 1 2 plus o eia
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HIV-1 Testing and STI Diagnosis Protocol
2
HIV and STI Diagnosis Protocol
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Two rapid HIV-1 test kits (Determine, Abbott Laboratories; Unigold Recombigen HIV, Trinity Biotech PLC, Ireland) were used for HIV-1 testing. Discrepant results were verified with a third-generation enzyme-linked immunosorbent assay test (Genetic System HIV-1/2 plus O EIA; Bio-Rad Laboratories, Redmond, WA). Genital syndromes diagnosed included urethritis (defined as 5 or more polymorphonuclear cells [PMNs] per high-power field on a Gram-stained urethral smear), proctitis (defined as 5 or more PMNs per high-power field on a Gram-stained rectal smear), or genital ulceration (any ulceration, either penile or perianal, on physical examination). Detection of Gram-negative, intracellular diplococci in either the urethra or rectum was used for diagnosis of gonococcal infection. All patients with laboratory-diagnosed STIs were traced and treated.
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