Human PDAC cell lines CFPAC-1, BXPC-3 and Capan-2, human umbilical vein endothelial cell line HUVEC and human embryonic kidney cell line 293T were purchased from the China Infrastructure of Cell Line Resources (Beijing). Cells passaged for less than 6 months were authenticated by DNA fingerprinting analysis using short-tandem repeat (STR) markers.
Bxpc 3
Manufactured by China Infrastructure of Cell Line Resources
Sourced in China
BxPC-3 is a pancreatic ductal adenocarcinoma cell line derived from a primary tumor. It is a commonly used model for pancreatic cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Cfpac 1, by China Infrastructure of Cell Line Resources (4 mentions)
Panc 1, by China Infrastructure of Cell Line Resources (4 mentions)
Sw1990, by China Infrastructure of Cell Line Resources (3 mentions)
Aspc1, by China Infrastructure of Cell Line Resources (2 mentions)
Hpde6 c7, by China Infrastructure of Cell Line Resources (2 mentions)
Interferon γ ifn γ, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using bxpc 3
1
Authentication of PDAC cell lines
2
Pancreatic Cancer Cell Culture and IFN-γ Treatment
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PANC-1, CFPAC-1, and BxPC-3 cells were obtained from China Infrastructure of Cell Line Resources. The cells were maintained in Dulbecco's modified Eagle's medium (Hyclone Co., Logan, UT, USA) supplemented with 10% fetal bovine serum (Hyclone Co., Logan, UT, USA) and 100 U/ml penicillin and streptomycin. The cells were grown at 37°C in a humidified 5% CO2 air incubator and subcultured twice a week. For treatment, the cells were washed twice with phosphate-buffered saline (PBS) and 1 × 106 cells were incubated in cell-culture plates with or without 500 U/ml interferon-γ (IFN-γ; Sigma Co., St. Louis, MO, USA) for 48 h.
3
Culturing and Maintaining Pancreatic Cancer Cell Lines
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Human pancreatic cancer cell line PANC-1, SW1990, and BxPC-3 were obtained from China Infrastructure of Cell Line Resources (Beijing, China). Capan-2 and MIA PaCa-2 were purchased from Obio Technology (Shanghai, China) Corp., Ltd. SW1990, PANC-1 and MIA PaCa-2 were cultured in DMEM, Capan-2 was maintained in McCoy’s 5A and BxPC-3 was cultured in RMPI 1640, all supplemented with 10% fetal bovine serum, 100 U/ml of penicillin and 100 μg/ml of streptomycin. All of the cells were incubated at 37 ℃ in a humidified atmosphere of 5% CO2. The gemcitabine (Gemzar) drug was purchased from Eli Lilly and Company (Indianapolis, Indiana, USA).
4
Culturing PC Cell Lines in Mice
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The PC cell lines Panc1, CFPAC1, Bxpc3, SW1990, AsPC1, and HPDE6-C7 were purchased from China Infrastructure of Cell Line Resources (Beijing, China) and cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Cleveland, OH, USA) supplemented with 10% fetal bovine serum at 37°C in an atmosphere containing 5% CO2. BALB/c male nude mice were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The mice experiments were approved by the Animal Experimental Ethics Committee of PLA General Hospital and conducted in the animal facility of PLA General Hospital according to the animal experimental ethics committee guidelines of PLA General Hospital.
5
Cultivation of Pancreatic Cell Lines
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Pancreatic cancer cell lines AsPC-1, BxPC-3, CFPAC-1, PANC-1, and SW1990 and human pancreatic duct epithelial cell line HPDE6-C7 were purchased from China Infrastructure of Cell Line Resources (Beijing, People’s Republic of China) and cultured in Dulbecco’s Modified Eagle’s Medium (Hyclone Co., Logan, UT, USA) and supplemented with 10% FBS (Hyclone Co.) at 37°C in a humidified 5% CO2 air incubator.
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