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Lactated ringer s solution

Manufactured by Abbott
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Sourced in Israel, United States

Lactated Ringer's solution is a sterile, isotonic electrolyte solution used in medical settings. It is primarily composed of sodium chloride, sodium lactate, potassium chloride, and calcium chloride in water. This solution is designed to help maintain fluid and electrolyte balance in the body.

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Lab products found in correlation

Euthanasia solution, by Vedco (2 mentions) Isoflurane, by Henry Schein (2 mentions) Dexdomitor, by Pfizer (1 mentions) Automacs magnetic cell separator, by Miltenyi Biotec (1 mentions)

5 protocols using lactated ringer s solution

1

In Vivo and Post-Mortem MRE of Porcine Brain

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Our study is based on one and the same brain of a 50.2 kg female Yorkshire pig. Prior to scanning, we sedated the animal with 6 mgAg intramuscular tiletamine zolazepam (Telazol, Fort Dodge, IA), which we induced with 3% isoflurane (Henry Schein Animal Health Dublin, OH) in oxygen delivered by a face mask. We intubated the trachea with an endotracheal tube, 8 mm in diameter, and maintained anesthesia with 1% to 3% isoflurane in oxygen using mechanical ventilation. To administer drugs and monitor the systemic arterial blood pressure, we placed percutaneous catheters in the left femoral vein and artery. We monitored heart rate, electrocardiogram, pulse oximetry, and end-tidal C02 throughout the imaging sequence. Throughout anesthesia, we intravenously administered lactated Ringer’s solution (Abbott Laboratories, Deerfield, IL) at a rate of 10 to 15 mL/kg per hour to compensate for fluid loss. We performed magnetic resonance elastography in vivo, then euthanized the animal with Euthanasia Solution (Vedco, Inc., St Joseph, MO), confirmed death by an electrocardiogram and auscultation, placed the animal back into the scanner, and performed post mortem magnetic resonance elastography in situ.
Weickenmeier J., Kurt M., Ozkaya E., de Rooij R., Ovaert T.C., Ehman R.L., Pauly K.B, & Kuhl E. (2018). Brain stiffens post mortem. Journal of the mechanical behavior of biomedical materials, 84, 88-98.
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2

Anesthesia protocol for sheep MRI studies

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Sheep were fasted for 24 hours prior to the study and then sedated with tiletamine and zolazepam (Telazol, Lederele Parenterals, Carolina, Puerto Rico) at 4 mg/kg, intramuscularly. Anesthesia was induced with a combination of 3% isoflurane in oxygen delivered by facemask and telazol in a continuous rate of infusion. All animals were orotracheally intubated and anesthesia was maintained with 1% to 3% isoflurane in oxygen with MRI conditional mechanical ventilation (Omni-Vent Series D, Allied Healthcare Products, St. Louis, MO) to maintain end-tidal carbon dioxide between 35 mm Hg and 45 mm Hg. Stomach tubes were placed after intubation to resolve gaseous distension and prevent regurgitation. Venous and arterial catheters were placed percutaneously for drug and fluid administration and blood pressure monitoring. Lactated Ringer’s solution (Abbott Laboratories, Abbott Park, IL) was administered intravenously at approximately 10 mL/kg/hr throughout anesthesia. The top of the head was shaved and treated with a depilatory cream for hair removal.
Gaur P., Casey K.M., Kubanek J., Li N., Mohammadjavadi M., Saenz Y., Glover G.H., Bouley D.M, & Pauly K.B. (2020). Histologic safety of transcranial focused ultrasound neuromodulation and magnetic resonance acoustic radiation force imaging in rhesus macaques and sheep. Brain stimulation, 13(3), 804-814.
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3

In Vivo and Post-Mortem MRE of Porcine Brain

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Our study is based on one and the same brain of a 50.2 kg female Yorkshire pig. Prior to scanning, we sedated the animal with 6 mgAg intramuscular tiletamine zolazepam (Telazol, Fort Dodge, IA), which we induced with 3% isoflurane (Henry Schein Animal Health Dublin, OH) in oxygen delivered by a face mask. We intubated the trachea with an endotracheal tube, 8 mm in diameter, and maintained anesthesia with 1% to 3% isoflurane in oxygen using mechanical ventilation. To administer drugs and monitor the systemic arterial blood pressure, we placed percutaneous catheters in the left femoral vein and artery. We monitored heart rate, electrocardiogram, pulse oximetry, and end-tidal C02 throughout the imaging sequence. Throughout anesthesia, we intravenously administered lactated Ringer’s solution (Abbott Laboratories, Deerfield, IL) at a rate of 10 to 15 mL/kg per hour to compensate for fluid loss. We performed magnetic resonance elastography in vivo, then euthanized the animal with Euthanasia Solution (Vedco, Inc., St Joseph, MO), confirmed death by an electrocardiogram and auscultation, placed the animal back into the scanner, and performed post mortem magnetic resonance elastography in situ.
Weickenmeier J., Kurt M., Ozkaya E., de Rooij R., Ovaert T.C., Ehman R.L., Pauly K.B, & Kuhl E. (2018). Brain stiffens post mortem. Journal of the mechanical behavior of biomedical materials, 84, 88-98.
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4

Anesthetic Protocols for Cross-Sectional Imaging in Dogs

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All dogs were induced using a propofol bolus to effect (2-6 mg/kg) for each of the two cross-sectional imaging events. The animals were orally intubated, placed in dorsal recumbency in a custom made trough for positioning in CT and MRI and maintained on isoflurane (Vaporizer set at 1-2%) and 100% oxygen using mechanical ventilation to an end-tidal CO2 level between 35-40mmHg. The dogs also received maintenance intravenous Lactated Ringer's Solution (5-10 ml/kg/hr; Abbott Laboratories, North Chicago, IL, USA) through the left cephalic catheter. Protocol A used fentanyl (Fentanyl Citrate, West-ward, Eatontown, NJ, USA) 5μg/kg bolus for premedication followed by 10μg/kg/hr continuous rate infusion (CRI) and midazolam (Midazolam, Hospira, Inc., Lake Forest, IL, USA) 0.2mg/kg bolus followed by 0.2mg/kg/hr CRI. Protocol B used dexmedetomedine (Dexdomitor, Pfizer Animal Health, New York, New York, USA) 1-2μg/kg bolus for premedication and 1-2μg/kg/hr CRI.
Drees R., Johnson R.A., Stepien R.L., Rio A.M., Saunders J.H, & François C.J. (2015). Quantitative planar and volumetric cardiac measurements using 64 MDCT and 3T MRI versus standard 2D and M-mode echocardiography: Does anesthetic protocol matter?. Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 56(6), 638-657.
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5

Isolation of Murine Bone Marrow Cells

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Cells were isolated from femurs and tibias of age-matched female donors. Bone marrow cells were subjected to T and B cell depletion by AutoMACS Magnetic Cell Separator (Miltenyi Biotec). Briefly, cells were incubated with anti-CD90.2 and anti-CD19 Miltenyi Biotec microbeads at 1:9 dilutions in AutoMACS buffer solution for 15 min at 4°C. Cells were then washed with AutoMACS buffer, resuspended at concentration of 108 cells per 500 μl, and passed through AutoMACS Magnetic Cell Separator columns using the ‘Deplete’ program. Collected cells were washed and resuspended at the concentration of 1.5 × 107 cells per 0.2 ml per mouse in lactated ringer’s solution (Abbott Laboratories).
Kim R.Y., Mangu D., Hoffman A.S., Kavosh R., Jung E., Itoh N, & Voskuhl R. (2017). Oestrogen receptor β ligand acts on CD11c+ cells to mediate protection in experimental autoimmune encephalomyelitis. Brain, 141(1), 132-147.
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