Cells were washed in phosphate-buffered saline (PBS) three times, incubated with 200 nM MitoTracker Deep Red (Beyotime Biotechnology) at 37 °C for 30 min, fixed with 4% paraformaldehyde for 30 min, and permeabilised with 0.1% Triton X-100 for 10 min. After blocking with 1% BSA for 1 h, the cells were incubated with diluted primary antibody (ALDH2 at 1:200 ratio) overnight at 4 °C. After washing, the cells were incubated with the corresponding FITC-conjugated secondary antibodies (1:200, 1 h at 37 °C). The nuclei were stained with 4′,6-diamidino-2-phenylindole for 5 min at room temperature. Immunofluorescence micrographs were captured using Nikon AXR confocal laser microscope.
Axr confocal laser microscope
Manufactured by Nikon
The Nikon AXR confocal laser microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes a laser light source and advanced optics to produce high-resolution, three-dimensional images of samples. The AXR offers precise control over parameters such as excitation wavelength, pinhole size, and scanning speed to optimize imaging for a variety of sample types and research needs.
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Lab products found in correlation
2 protocols using axr confocal laser microscope
1
Mitochondrial Protein Localization Imaging
2
Mitochondrial Membrane Potential Assay
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The mitochondrial membrane potential (ΔΨ m) was detected by JC-1 (Beyotime Biotechnology). Briefly, cells were washed twice in PBS, stained with JC-1 for 20 min at 37 °C, washed twice with binding buffer, and imaged using a Nikon AXR confocal laser microscope.
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