Uk5099

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom

UK5099 is a laboratory equipment product manufactured by Merck Group. It serves as a core functional component for various scientific applications. The device specifications and capabilities are maintained in a factual and unbiased manner, without any interpretations or extrapolations on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Etomoxir, by Merck Group (11 mentions) Bptes, by Merck Group (11 mentions) Rotenone, by Merck Group (8 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (6 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Antimycin a, by Merck Group (5 mentions) Oligomycin, by Merck Group (3 mentions) Bam15, by Merck Group (3 mentions) Sterile cell culture material, by Sarstedt (3 mentions) Etomoxir, by Cayman Chemical (3 mentions) Ar c155858, by Bio-Techne (3 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (3 mentions) 2 deoxyglucose 2 dg, by Merck Group (2 mentions) Penicillin g streptomycin sulfate solution, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Fetal calf serum (fcs), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Etomoxir, by Selleck Chemicals (2 mentions) Mitotempo, by Merck Group (2 mentions) Oligomycin, by Agilent Technologies (2 mentions)

60 protocols using uk5099

Effects of UK5099 and Aspartate on Neural Stem Cell Quiescence and Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proliferative NSPCs were treated with either 1 or 10 μM UK5099 (no. PZ0160, Sigma-Aldrich) for 72 hours. For quiescence experiments, NSPCs were treated with 1 or 10 μM UK5099 (no. PZ0160, Sigma-Aldrich), 1 μM lactate dehydrogenase A inhibitor (GSK 2837808A, Tocris, 5189/10), and 1, 2.5, or 5 mM aspartate (no. 1690.2, Roth) simultaneously with quiescence induction. For exit experiments, quiescent NSPCs were treated with UK5099 after establishment of quiescence.
For differentiation experiments, proliferative NSPCs were treated with 1 or 10 μM UK5099 for 48 hours. Then, cells were washed off and plated (65,000 cells/cm²) on coated coverslips in media containing one-fifth of the growth factors and indicated UK5099 concentration. Similar to UK5099 treatment, 1, 2.5, or 5 mM aspartate (no. 1690.2, Roth) was used during the induction of quiescence.

Petrelli F., Scandella V., Montessuit S., Zamboni N., Martinou J.C, & Knobloch M. (2023). Mitochondrial pyruvate metabolism regulates the activation of quiescent adult neural stem cells. Science Advances, 9(9), eadd5220.

+ Open protocol

+ Expand

Modulating Cell Signaling Pathways

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human LAC cell lines A549, H1299, H1975, and human normal bronchial epithelial (HBE) cells were purchased from the ATCC (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 100 U/ml penicillin–streptomycin (HyClone, Logan, UT, USA). The cells were maintained in a humidified 37 °C incubator with a 5% CO₂ atmosphere. UK5099 (Sigma-Aldrich, St. Louis, MO, USA), a MPC1 inhibitor, cells were treated with UK5099 with 40 μM for 48 h^{6 (link)}. IL-6 (PeproTech, Rocky Hill, NJ, USA), a STAT3 specific agonist, cells were treated with indicated dose for 24 h.

Zou H., Chen Q., Zhang A., Wang S., Wu H., Yuan Y., Wang S., Yu J., Luo M., Wen X., Cui W., Fu W., Yu R., Chen L., Zhang M., Lan H., Zhang X., Xie Q., Jin G, & Xu C. (2019). MPC1 deficiency accelerates lung adenocarcinoma progression through the STAT3 pathway. Cell Death & Disease, 10(3), 148.

+ Open protocol

+ Expand

Modulation of Cytokine Release by Metabolic Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated ATMs were cultured in RPMI 1640 (Lonza, Verviers, Belgium) supplemented with 10% (vol./vol.) FCS and 1% (vol./vol.) penicillin/streptomycin (PS) (RPMI/FCS/PS) for 24 h (200,000 cells/well). The contribution of various metabolic pathways to cytokine release was examined by providing 5.5 mmol/l 2-deoxy glucose (2-DG) (Merck, Darmstadt, Germany), 50 μmol/l Etomoxir (Merck), 10 μmol/l UK5099 (Merck) or 10 μmol/l BPTES (Merck) 2 h after plating until the end of the 24 h culture period.

Boutens L., Hooiveld G.J., Dhingra S., Cramer R.A., Netea M.G, & Stienstra R. (2018). Unique metabolic activation of adipose tissue macrophages in obesity promotes inflammatory responses. Diabetologia, 61(4), 942-953.

+ Open protocol

+ Expand

Metabolic Inhibitor Procurement and Usage

Check if the same lab product or an alternative is used in the 5 most similar protocols

2-Deoxy-d-glucose (2DG), antimycin A, BAM15, fetal calf serum (FCS), oligomycin and rotenone were purchased from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). Bovine serum albumin, dimethyl sulfoxide (DMSO), perchloric acid, NAD + , NADH and NADP + were from AppliChem (Darmstadt, Germany; RRID:SCR_005814). β-lapachone was from Abcam (Berlin, Germany; RRID:SCR_012931) and WST1 from Dojindo (Munich, Germany). Etomoxir and UK5099 were obtained from Merck (Darmstadt, Germany; RRID:SCR_001287). Dulbecco's modified Eagles medium (DMEM) and penicillin G/streptomycin sulfate solution were obtained from Thermo Fisher Scientific (Schwerte, Germany; RRID:SCR_008452). The Cell Titer Glo® 2.0 ATP Assay Kit was obtained from Promega (Walldorf, Germany; RRID:SCR_006724). ATP and the enzymes used for assays to quantify lactate, glucose, 2DG and 2DG6P were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Carl Roth (Karlsruhe, Germany; RRID:SCR_005711), Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland). Sterile cell culture materials as well as transparent and black microtiter plates were obtained from Sarstedt (Nümbrecht, Germany).

Harders A.R., Watermann P., Karger G., Denieffe S.C., Weller A., Dannemann A.C., Willker J.E., Köhler Y., Arend C, & Dringen R. (2024). Consequences of a 2-Deoxyglucose Exposure on the ATP Content and the Cytosolic Glucose Metabolism of Cultured Primary Rat Astrocytes. Neurochemical research.

+ Open protocol

+ Expand

Pyruvate Carrier Inhibition Impacts Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GLp strain was cultured in 150 mL of UAB medium with 6% (v/v) glycerol in a 1.3-L bioreactor (BioFlo, Eppendorf, Germany) at pH 5.0 and 30 °C, starting at an OD₆₀₀ of 0.5. The compound 2-Cyano-3-(1-phenyl-1H-indol-3-yl)-2-propenoic acid, commercially known as UK-5099 (PZ0160-5MG, Sigma-Aldrich), known to inhibit the activity of the mitochondrial pyruvate carrier [32 (link)], was added to the culture after 15 h at a concentration of 20 μM. Samples of 1 mL were then collected periodically for further analysis. Experiments were conducted in duplicate.

de Oliveira Junqueira A.C., Moreira Melo N.T., Skorupa Parachin N, & Costa Paes H. (2023). Loss of a Functional Mitochondrial Pyruvate Carrier in Komagataella phaffii Does Not Improve Lactic Acid Production from Glycerol in Aerobic Cultivation. Microorganisms, 11(2), 483.

+ Open protocol

+ Expand

Cell Culture and Metabolic Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

HUH7 were obtained from JCRB, BxPC3 and Panc03.27 were obtained from ATCC. STR profiling and routine testing for mycoplasma contamination were performed. HUH7 were grown in DMEM, 10% FCS, BxPC3 were grown in RPMI-1640, 10% FCS and Panc03.27 were grown in RPMI-1640, 15% FCS, 10 Units/ml human recombinant insulin (PAN-Biotech GmbH, Aidenbach, Germany & Sigma-Aldrich). All cells were cultured under constant humidity at 37 °C, 5% CO₂. For HUH7, all plastic ware was pre-coated with 0.001% collagen G (PBS). Archazolid A was provided by Rolf Müller, Torin1, CCCP, BPTES, UK5099 and Etomoxir were purchased from Sigma-Aldrich, and dissolved in DMSO (Sigma-Aldrich).

Bartel K., Pein H., Popper B., Schmitt S., Janaki-Raman S., Schulze A., Lengauer F., Koeberle A., Werz O., Zischka H., Müller R., Vollmar A.M, & von Schwarzenberg K. (2019). Connecting lysosomes and mitochondria – a novel role for lipid metabolism in cancer cell death. Cell Communication and Signaling : CCS, 17, 87.

+ Open protocol

+ Expand

Metabolic Profiling of HaCaT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The various HaCaT cell lines were seeded at 2.0E4/well in Seahorse-XF96 microplates (Agilent, Waldbronn, Germany) and grown in complete DMEM for 48 h. Next, cells were equilibrated in Krebs Henseleit buffer (111 mM NaCl, 4.7 mM KCl, 1.25 mM CaCl 2 , 2 mM MgSO 4 , 1.2 mM Na 2 HPO 4 ) supplemented with 5 mM L-glucose and 1 mM L-glutamine, for 30 min at 37 °C at ambient CO 2 and the cellular oxygen consumption rate (OCR) was analysed using Seahorse 96 extracellular flux analyser (Agilent) as previously described [73] (link) UK5099 (PZ0160; Sigma-Aldrich) and 5 lM Etomoxir (11969; Cayman Chemicals, Ann Arbor, MI, USA) have been used to determine the cells' metabolic reliance on glutamine, pyruvate or fatty acids, respectively.

Nold S.P., Sych K., Imre G., Fuhrmann D.C., Pfeilschifter J., Vutukuri R., Schnutgen F., Wittig I., Meisterknecht J., Frank S, & Goren I. (2023). Reciprocal abrogation of PKM isoforms: contradictory outcomes and differing impact of splicing signal on CRISPR/Cas9 mediates gene editing in keratinocytes. The FEBS journal, 290(9).

+ Open protocol

+ Expand

Mitochondrial Nutrient Inhibition in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured under standard conditions of atmospheric O2, 5% CO2 and 37°C in DMEM (Thermo Fisher Scientific #10567022) supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific), 50 μg/mL uridine (Sigma-Aldrich), 1% MEM non-essential amino acids (Thermo Fisher Scientific), and 10 μM-1.7 μM palmitate-BSA conjugate (Sigma-Aldrich). Treatment with dexamethasone (Dex, Sigma-Aldrich #D4902, 100 nM) and Dex plus the Mitochondrial Nutrients Uptake InhibiTors cocktail (Dex+mitoNUITs) began after 15-days of culturing post-thaw to allow the cells to adjust to the in vitro environment and were dosed every passage. The mitoNUITs cocktail included i) UK5099 (Sigma-Aldrich #PZ0160, 2 μM), an inhibitor of the mitochondrial pyruvate carrier (MPC) that interferes with the pyruvate import into the mitochondrial matrix ii) Etomoxir (Sigma-Aldrich #E1905, 4 μM), an inhibitor of carnitine palmitoyltransferase-1 (CPT-1) that interferes with the fattyacid-derived Acyl-CoA import into the mitochondrial matrix; and iii) BPTES (Sigma-Aldrich #SML0601, 3 μM), an inhibitor of glutaminase 1 (GLS1) that prevents the conversion of glutamine to glutamate into the mitochondrial matrix. The combined action of these three compounds ultimately abates the availability of the tricarboxylic acid cycle (TCA) to produce Acetyl-CoA.

Bobba-Alves N., Sturm G., Lin J., Ware S.A., Karan K.R., Monzel A.S., Bris C., Procaccio V., Lenaers G., Higgins‐Chen A., Levine M.E., Horvath S., Santhanam B., Kaufman B.A., Hirano M., Epel E.S., & Picard M. (2022). Cellular Allostatic Load is linked to Increased Energy Expenditure and Accelerated Biological Aging.

+ Open protocol

+ Expand

HepG2, 293FT, and BMDM Cell Culture

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HepG2 cells were obtained from American Type Culture Collection (ATCC) and cultured according to ATCC recommendations. Cells were maintained in MEM with 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin. For biological validation experiments, 3.0 × 10⁶ cells were seeded in 10 cm dishes. After 48 h., cells were treated with 1 mM sodium propionate (Sigma-Aldrich), 1 mM cyclopropanecarboxylic acid (Sigma-Aldrich), 10 μM PZ-2891 (MedChem Express), or 5 μM UK5099 (Sigma-Aldrich) for 24 h. and harvested for extractions. The 293FT cell line was obtained from Invitrogen and maintained in DMEM with 10% FBS and 1% penicillin/streptomycin. For acyl CoA extractions, 2.0 × 10⁶ cells were seeded in 10 cm dishes and harvested after 72 h. Bone marrow-derived macrophages (BMDMs) were harvested by flushing the tibiae and femurs of 8–12-week male mice. Following red blood cell lysis, cells were centrifuged at 364× g for 5 min and resuspended in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin/streptomycin, and a 5% (v/v) CMG-conditioned medium [42 (link)]. BMDMs were differentiated for 6 days and 5.0 × 10⁶ BMDMs were then re-plated in 10 cm dishes. After 48 h., new medium containing 20 ng/mL IL-4 (Peprotech) alone or IL-4 with 200 μM etomoxir was added to cells for 24 h. before extraction.

Jones A.E., Arias N.J., Acevedo A., Reddy S.T., Divakaruni A.S, & Meriwether D. (2021). A Single LC-MS/MS Analysis to Quantify CoA Biosynthetic Intermediates and Short-Chain Acyl CoAs. Metabolites, 11(8), 468.

+ Open protocol

+ Expand

Mitochondrial Staining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

The nuclear dye Hoechst 33342 was purchased from Life Technologies. Ultrapure LPS (E. coli 0111:B4) was purchased from In vivogen. MitoTEMPO and UK-5099 were purchased from Sigma Aldrich (SML0737; PZ0160). Etomoxir was purchased from Selleckchem (S8244). MitoTracker Green, MitoTracker DeepRed and MitoSOX Red were purchased from Thermo Fisher Scientific (M7514; M22426; M36008).

Røst L.M., Louet C., Bruheim P., Flo T.H, & Gidon A. (2022). Pyruvate Supports RET-Dependent Mitochondrial ROS Production to Control Mycobacterium avium Infection in Human Primary Macrophages. Frontiers in Immunology, 13, 891475.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!