Duloxetine hydrochloride (cat. no. 040-34071, Wako Pure Chemicals) was dissolved in dimethyl sulfoxide (DMSO) at a final concentration of 10 mM and stored at −20°C. Paroxetine hydrochloride (cat. no. 168-24431, Wako Pure Chemicals), desloratadine (cat. no. D3787, TCI Chemicals), promethazine, amitriptyline hydrochloride (cat. no. 013-12882, Wako Pure Chemicals), and chlorpromazine hydrochloride (cat. no. 033-10581, Wako Pure Chemicals) were dissolved in DMSO at a concentration of 10 mM and stored at −20°C.
Chlorpromazine hydrochloride
Manufactured by Fujifilm
Sourced in Japan
Chlorpromazine hydrochloride is a chemical compound used as a laboratory reagent. It is a white to pale yellow crystalline powder that is soluble in water and alcohol. The compound is typically used in pharmaceutical research and development applications.
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Lab products found in correlation
Dopamine hydrochloride, by Nacalai Tesque (1 mentions)
Bovine serum albumin (bsa), by Fujifilm (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Ovalbumin (ova), by Fujifilm (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Bistris, by Dojindo Laboratories (1 mentions)
Human alpha1 acid glycoprotein, by Merck Group (1 mentions)
Glycine, by Fujifilm (1 mentions)
Coelenterazine, by Fujifilm (1 mentions)
5 protocols using chlorpromazine hydrochloride
1
Preparation of Psychoactive Compound Solutions
2
Oral Administration of Methylhydroxyprogesterone
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MH (the purity was 99.8%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). MH was dissolved in 0.5 w/v% carboxymethyl cellulose solution (0.5% CMC) to reach a concentration of 5, 8, and 10 mg/ml for administration to the 25, 40, and 50 mg/kg/day groups, respectively. Chlorpromazine hydrochloride (the purity was 99% or more), which was used to suppress intestinal peristalsis, was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and dissolved in 0.5 w/v% methyl cellulose solution to reach a concentration of 5 mg/ml.
3
Evaluating Aggression in Lasius japonicus Workers
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We examined the effects of the 4 amines and a DA antagonist on the aggressiveness of L. japonicus workers. For DA (dopamine hydrochloride, NACALAI TESQUE, INC., Kyoto, Japan), 5HT (serotonin–creatinine sulfate monohydrate, Wako, Osaka, Japan), and the DA antagonist (CP; chlorpromazine hydrochloride, Wako, Osaka, Japan), we prepared a dilution series (10, 1, 0.1, 0.01, 0.001 mM) of each chemical in 10% sucrose solution with a few amounts of neutral red (NR; Wako, Osaka, Japan), and the CP solution was adjusted to include 10 mM DA. The effects of 10 mM TA (tyramine hydrochloride, Wako, Osaka, Japan) and OA (octopamine hydrochloride, TOKYO CHEMICAL INDUSTRY, Tokyo, Japan) were also examined. Aggressiveness was measured for each ant, and it introduced into a 0.6 ml tube with 20 μl of each solution absorbed in a cotton ball (approximately 5 mm in diameter) placed in the inside of the cap. The tubes were left 24 h at 25 °C. The aggressiveness of the ant was recorded using the same method above. After recording, each ant was crushed on filter paper, and the intake of the chemical was confirmed by the red color. When intake was not confirmed, we removed such data from the analyses. To confirm the effects of 10% sucrose or NR, the same experiment was conducted using both solutions. In each experiment, 15 ants without contact with the aphids from 2 ~ 3 shoots (= ant colonies) were used.
4
Bioluminescent Protein Assay Reagents
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The commercially available materials used in this study were obtained from the following commercial suppliers. Human alpha 1-acid glycoprotein (alpha 1-acid glycoprotein from human plasma; lot number: SLBJ6840V) was from Sigma-Aldrich (St. Louis, MO, USA). OVA (albumin from egg; lot number: CAL1231), BSA (albumin from bovine serum, fatty acid/IgG/protease free; lot number: CAQ4454), coelenterazine, chlorpromazine hydrochloride, Tris-HCl buffers, glycine, sodium hydroxide, and hydrochloric acid were from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). HSA (albumin, human serum, F-V; lot number: M8H7013) was from Nacalai Tesque (Kyoto, Japan). Cypridina luciferin was from ATTO Corporation (Tokyo, Japan). BisTris was from Dojindo Laboratories. All materials were used without further purification. A recombinant CLase from C. noctiluca was prepared according to the method reported previously [12 (link)].
5
Intestinal Peristalsis Suppression Protocol
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FM (the purity is 95 % or more) was purchased from Cayman Chemical (MI, USA). FM was dissolved in saline to reach a concentration of 20 mg/ml. Chlorpromazine hydrochloride (the purity is 99 % or more), which was used to suppress peristalsis of the intestinal tract, was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Chlorpromazine hydrochloride was dissolved in 0.5 w/v% methyl cellulose solution to reach a concentration of 5 mg/ml.
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