Senescence associated β galactosidase sa β gal staining kit

Manufactured by Beyotime

Sourced in China

The Senescence-associated β-galactosidase (SA-β-gal) staining kit is a laboratory tool designed to detect and quantify cellular senescence. The kit provides the necessary reagents and protocols to stain cells that exhibit increased lysosomal β-galactosidase activity, a hallmark of the senescent phenotype. The kit enables researchers to assess the level of cellular senescence in various cell types and experimental settings.

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Close spelling variants of this product

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14 protocols using senescence associated β galactosidase sa β gal staining kit

Senescence-associated β-galactosidase Assay

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Cell senescence was measured with the senescence-associated β-galactosidase (SA-β-gal) staining kit (Beyotime) according to the manufacturer’s protocol. All experiments were repeated three times.

Guo X., Lee S, & Cao P. (2019). The inhibitive effect of sh-HIF1A-AS2 on the proliferation, invasion, and pathological damage of breast cancer via targeting miR-548c-3p through regulating HIF-1α/VEGF pathway in vitro and vivo. OncoTargets and therapy, 12, 825-834.

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Senescence-Associated β-Galactosidase Assay

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The NPC senescence was evaluated by senescence-associated β-galactosidase (SA-β-gal) staining kit (Beyotime) according to the protocols. Briefly, cells implanted into six-well plates were fixed with 0.2% glutaraldehyde for 10 min at room temperature, and subsequently stained with X-gal staining solution overnight at pH 6.0. The aging NPCs were positive for SA-β-gal staining and quantified for statistical analysis.

Wang Y., Zuo R., Wang Z., Luo L., Wu J., Zhang C., Liu M., Shi C, & Zhou Y. (2019). Kinsenoside ameliorates intervertebral disc degeneration through the activation of AKT-ERK1/2-Nrf2 signaling pathway. Aging (Albany NY), 11(18), 7961-7977.

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Senescence-associated β-Galactosidase Assay

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According to the instructions of the manufacturer, the Senescence-associated β-Galactosidase (SA-β-gal) Staining Kit (Beyotime, Shanghai, China) was used to assay senescence. NP cells were fixed at room temperature for 15 min and washed twice with PBS. Next, we added 1 mL of β-galactosidase staining solution (10 μL of staining solution A, 10 μL of staining solution B, 930 μL of staining solution C, and 50 μL of X-Gal solution) to the wells and transferred the plates to an incubator (no CO₂) at 37°C at least overnight. Under an inverted light microscope (EVOS-FL Cell Imaging System, Thermo Fisher Scientific), aged NP cells with high SA-β-gal activity were blue.

Zhang G.Z., Chen H.W., Deng Y.J., Liu M.Q., Wu Z.L., Ma Z.J., He X.G., Gao Y.C, & Kang X.W. (2022). BRD4 Inhibition Suppresses Senescence and Apoptosis of Nucleus Pulposus Cells by Inducing Autophagy during Intervertebral Disc Degeneration: An In Vitro and In Vivo Study. Oxidative Medicine and Cellular Longevity, 2022, 9181412.

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Senescence Detection in BMSCs

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A senescence-associated β-galactosidase (SA-β-gal) staining kit (Beyotime, China) was used to detect cell senescence of BMSCs. Passage 4 BMSCs were incubated in a six-well plate. After being transfected with siRNA, the medium was removed and cells were rinsed with PBS twice. Then, BMSCs were fixed with 4% paraformaldehyde and stained with a working mixture of β-galactosidase and X-Gal at 37 °C overnight in dark. Senescent cells were inspected with an optical microscope and counted in random field of vision.

Deng S., Qi M., Gong P, & Tan Z. (2022). The circadian clock component BMAL1 regulates osteogenesis in osseointegration. Frontiers in Pediatrics, 10, 1091296.

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Senescence Evaluation in NP Cells

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The level of senescence was measured by senescence-associated β-galactosidase (SA-β-gal) staining kit (Beyotime, Shanghai, China) according to the instruction. Aging NP cells showing higher SA-β-gal activity were stained blue.

Chen J., Xie J.J., Jin M.Y., Gu Y.T., Wu C.C., Guo W.J., Yan Y.Z., Zhang Z.J., Wang J.L., Zhang X.L., Lin Y., Sun J.L., Zhu G.H., Wang X.Y, & Wu Y.S. (2018). Sirt6 overexpression suppresses senescence and apoptosis of nucleus pulposus cells by inducing autophagy in a model of intervertebral disc degeneration. Cell Death & Disease, 9(2), 56.

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Senescence-Associated β-Galactosidase Assay

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The senescence of cells was assessed using the senescence-associated-β-GalactoSidase (SA-β-gal) staining kit (Beyotime, Shanghai, China). The senescent chondrocytes are stained blue to indicate the higher SA-β-gal activity.

Guo Q., Chen X., Chen J., Zheng G., Xie C., Wu H., Miao Z., Lin Y., Wang X., Gao W., Zheng X., Pan Z., Zhou Y., Wu Y, & Zhang X. (2021). STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-κB signaling pathway. Cell Death & Disease, 12(1), 13.

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Senescence-Associated β-Galactosidase Assay

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The senescent status was demonstrated by the senescence-associated β-galactosidase (SA-β-gal) staining kit (Beyotime). Cells were fixed on the plate and incubated with the mixed staining solutions for 24 h at 37°C.

Zhu Q., Dong Q., Wang X., Xia T., Fu Y., Wang Q., Wu R, & Wu T. (2022). Palmitic Acid, A Critical Metabolite, Aggravates Cellular Senescence Through Reactive Oxygen Species Generation in Kawasaki Disease. Frontiers in Pharmacology, 13, 809157.

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Cellular Senescence Detection by SA-β-gal Staining

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Cellular senescence was detected by senescence‐associated β‐galactosidase (SA‐β‐gal) staining kit (Beyotime, China). PC12 cells were plated in 6‐well plates. After grouped and treated as described above, the cells were stained with β‐galactosidase staining solution and incubated overnight at 37°C (free CO₂). The number of positive cells was calculated under a light microscope (IX‐70, Olympus, Tokyo, Japan).

Zhu L., Liu Y., Wu X., Ren Y., Zhang Q., Ren L, & Guo Y. (2021). Cerebroprotein hydrolysate‐I protects senescence‐induced by D‐galactose in PC12 cells and mice. Food Science & Nutrition, 9(7), 3722-3731.

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Senescence Assay of Doxorubicin-Treated Cells

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According to instructions for the Senescence‐associated‐β‐Galactosidase (SA‐β‐Gal) Staining Kit (Beyotime), LY8 cells treated with 10, 20, and 40 nM doxorubicin respectively were fixed with galactosidase fixative and incubated in dyeing working fluid as previously described.¹⁶ Finally, the stained cells were observed under a microscope (CNOPTEC). Cells that stained green‐blue were evaluated as positive senescent cells. All SA‐β‐gal assays reflect at least three samples.

Wang J., Tao Q., Pan Y., Wanyan Z., Zhu F., Xu X., Wang H., Yi L., Zhou M, & Zhai Z. (2020). Stress‐induced premature senescence activated by the SENEX gene mediates apoptosis resistance of diffuse large B‐cell lymphoma via promoting immunosuppressive cells and cytokines. Immunity, Inflammation and Disease, 8(4), 672-683.

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Senescence-Associated β-Galactosidase Assay

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The level of senescence was measured by senescence associated β-galactosidase (SA-β-gal) staining kit (Beyotime, Shanghai, China) according to the instruction. Aging chondrocytes showing higher SA-β-gal activity were stained blue.

Zheng G., Zhan Y., Li X., Pan Z., Zheng F., Zhang Z., Zhou Y., Wu Y., Wang X., Gao W., Xu H., Tian N, & Zhang X. (2018). TFEB, a potential therapeutic target for osteoarthritis via autophagy regulation. Cell Death & Disease, 9(9), 858.

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