A total of 2.3 × 104 cells of each cell type, fibroblasts, AdMSC, and WJ-MSC were seeded per 9 cm2 well. They were cultured in DMEM/Ham-F12 supplemented with 5% PL, 5% PLS, or 10% FBS, together with 100 U/mL penicillin, 100 mg/mL streptomycin, and 1% L-glutamine. In the medium supplemented with PL, 1% sodium heparin (Fresenius Kabi, Toronto, ON, Canada) at 5000 IU/mL was added. Photographic records were taken at 4, 24, 48, 72, and 96 h, and 3 photos were taken for each well. Images were analyzed with Fiji, ImageJ software (2.9.0 version, Fiji). The assay was performed in triplicate.
Sodium heparin
Manufactured by Fresenius
Sourced in Canada, United States
Sodium heparin is an anticoagulant agent used in laboratory settings to prevent blood clotting in collected samples. It acts by inhibiting the formation of thrombin, a key enzyme involved in the blood coagulation process. Sodium heparin is commonly used in various types of laboratory equipment and procedures requiring blood analysis or processing.
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Lab products found in correlation
4 protocols using sodium heparin
1
Comparative Cell Culture Optimization
2
Sheep ECLS Model with Pediatric MLung
3
Vascular Imaging of Murine Hindlimb
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The mice were euthanized at day 21, and a catheter was inserted into the left ventricle of the mice. The mouse vasculature was flushed with PBS supplemented with 100 U/mL heparin sodium (Fresenius Kabi) and then 4% PFA. One to two milliliters of Microfil MV-120 (Flow Tech, Inc.) was injected into the left ventricle to increase contrast of vasculature. The leg muscles from both limbs were separately collected and imaged with a micro-CT imaging system (VivaCT40; Scanco Medical) using a voxel size of 10.5 mm, X-ray source voltage of 55 kVp, and 145 mA current. The micro-CT images were analyzed using a matching three-dimensional segmentation algorithm of thresholding.
4
Anticoagulant Comparison in Bone Marrow Aspiration
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For all donors participating in the study, two 50 mL BMA draws were performed where each draw contained one of the two respective anticoagulants. Syringes were labeled arbitrarily as to blind the physician from the identity of the anticoagulant. For each donor, the bone marrow was aspirated bilaterally from the posterior iliac spine; anticoagulant used for the first aspiration was randomized. SC (sodium citrate 40 mg/mL stock solution, compounded Fagron Sterile Services, USA) was used in all cases at 15% final v./v. of the stock solution and HS (heparin sodium, Fresenius Kabi, USA) was used at two separate concentrations, 1,000 units/mL (U/mL) and 100 U/mL final v./v. (Series 1 and 2, respectively); 1 mg HS is ~120-140 U/mL [22 (link)]. All syringes and 11-gauge trocars were briefly rinsed/coated with HS solution before anticoagulants were loaded prior to BMA aspiration.
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