Agilent 5110

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 5110 is an inductively coupled plasma optical emission spectrometer (ICP-OES) designed for elemental analysis. It provides quantitative determination of trace elements in a wide range of sample types, including environmental, food, and geological samples. The 5110 employs a vertical plasma configuration and an echelle optics system to deliver high-performance multi-element analysis.

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28 protocols using agilent 5110

Degradation Kinetics of Chitosan Scaffolds

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The scaffolds (diameter = 6 mm, height = 2 mm) and CS coating (5 × 5 cm, 200 mg) were subjected to vacuum drying, and the initial mass was recorded as M0. Subsequently, the scaffolds were immersed in 0.05 M Tris-HCL buffer (pH = 7.4), and the CS coatings were, respectively, immersed in a 0.1 M NH₄HCO₃ buffer (pH = 7.4) with or without lipase (1.25 mg/mL). The proportions used in the above experiments was 200 mg/mL. The solution was refreshed every 2 days, and the scaffolds and CS coating were weighed and recorded as Mt after washing with PBS and vacuum-drying at the designated time point (3rd, 7th, 14th, and 21st days). The weight loss ratio is calculated as follows:

W e i g h t L o s s (%) = \frac{(M_{0} - M_{t})}{M_{0}} \times 100

The ion concentrations of Mg²⁺ and PO₄³⁻ were measured simultaneously at the specified time points (3rd, 7th, 14th, and 21st days) using an inductively coupled plasma optical emission spectrometer (ICP-OES, Agilent 5110, Santa Clara, CA, USA).

Gong C., Yang J., Zhang X., Wei Z., Wang X., Huang X., Yu L, & Guo W. (2023). Functionalized Magnesium Phosphate Cement Induces In Situ Vascularized Bone Regeneration via Surface Lyophilization of Chondroitin Sulfate. Biomedicines, 12(1), 74.

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Quantifying Matrix-Associated Iron in Biofilm

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For estimating the matrix associated iron [26 (link)] 3-day old colony biofilm was dispersed in 15 mL PBS, sonicated, and centrifuged at 14,000 rpm for 1 min, the supernatant was collected and filtered using 0.22 µ filter. Fe content in the samples was estimated at 238 nm using ICP-OES Agilent 5110. The statistical test used for analysis is unpaired t-test using GraphPad Prism5.

R. K.B., S. S.C, & N S.S. (2023). “Sharing the matrix” – a cooperative strategy for survival in Salmonella enterica serovar Typhimurium. BMC Microbiology, 23, 230.

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Microwave-Assisted Elemental Analysis

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Similarly as described before,⁶⁵ the as-prepared nanoparticles were weighted into PTFE tubes and mixed with 6 mL 65% HNO₃ p.a. (Merck) and 1 mL 30% H₂O₂ p.a. (Merck). The samples were then digested in a microwave and elements were quantified with an ICP-OES (Agilent 5110, Santa Clara, CA, USA). Due to the high silicate content and thus incomplete Si dissolution, the Si percentage was underestimated in some samples. For these samples, the residual mass was assumed to be Si as well.

Matter M.T., Doppegieter M., Gogos A., Keevend K., Ren Q, & Herrmann I.K. (2021). Inorganic nanohybrids combat antibiotic-resistant bacteria hiding within human macrophages. Nanoscale, 13(17), 8224-8234.

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Elemental Analysis of Plant Roots

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Approximately 0.15 g of dried root tissue powder was transferred into a beaker containing 4 mL of nitric acid overnight. The mixture was heated on a stirring hotplate at 200 °C for 3 h, and 2 mL of H₂O₂ was gradually added during the digestion process, with the solution evaporating to 1 mL using residual heat. The solution was deliquated to 10 mL with Milli Q water and then filtered through 0.22 μm filters to quantify the element content of Magnesium (Mg), Iron (Fe), Copper (Cu), and Zinc (Zn) via inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-OES) (Agilent 5110, Agilent, Santa Clara, CA, USA).

Su D., Li W., Zhang Z., Cai H., Zhang L., Sun Y., Liu X, & Tian Z. (2024). Discrepancy of Growth Toxicity of Polystyrene Nanoplastics on Soybean (Glycine max) and Mung Bean (Vigna radiata). Toxics, 12(2), 155.

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Comprehensive Characterization of Nanoparticles

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The morphology and structure of the as-prepared nanoparticles were examined by transmission electron microscopy (TEM; JEM-2100; JEOL, Tokyo, Japan). Nitrogen adsorption/desorption isotherms and pore size distributions were obtained with an adsorption analyzer (Micromeritics, Norcross, GA, USA). Fourier-transform infrared spectra were measured on a Nexus 670 spectrometer (Thermo Fisher Scientific). The wide-angle X-ray diffraction (XRD) patterns were obtained on a D8 ADVANCE powder diffractometer using Cu K_α1 radiation (1.5405 Å). Dynamic light scattering was conducted by using a BI-200SM multiangle dynamic/static laser scattering instrument (Brookhaven Instruments Corporation, Holtsville, NY, USA). Zeta potential was detected by a Zetasizer Nano ZS Nanosizer (Malvern Instruments, Malvern, UK). UV-Vis spectra were recorded on a UV-3101PC Shi-madzu spectroscope (Shimadzu, Kyoto, Japan). The content of Si was determined by Agilent 5110 inductively coupled plasma-optical emission spectroscopy (Agilent Technologies, Santa Clara, CA, USA).

Fang J., Liu Y., Chen Y., Ouyang D., Yang G, & Yu T. (2018). Graphene quantum dots-gated hollow mesoporous carbon nanoplatform for targeting drug delivery and synergistic chemo-photothermal therapy. International Journal of Nanomedicine, 13, 5991-6007.

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Sulfur Content Analysis in Plant Tissues

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Sulfur was measured for 21-d-old shoot and root dry samples by digestion method, using 2 mL freshly prepared 1% HNO₃ (trace metal grade, Fisher Scientific) that was added to the preweighed shoot and root samples. After digestion, 1 mL of samples were transferred to volumetric test tubes and diluted to a final volume of 50 mL with 1% HNO₃. The concentration of sulfur was determined using ICP-OES (Agilent 5110, Agilent Technologies) with the following conditions: 1.2 KW RF power, 0.7 L/min nebulizer flow, 12.0 L/min plasma flow, 1.0 L/min auxiliary flow, argon gas, 8-mm viewing height, and axial viewing mode.

Andrés-Barrao C., Alzubaidy H., Jalal R., Mariappan K.G., de Zélicourt A., Bokhari A., Artyukh O., Alwutayd K., Rawat A., Shekhawat K., Almeida-Trapp M., Saad M.M, & Hirt H. (2021). Coordinated bacterial and plant sulfur metabolism in Enterobacter sp. SA187–induced plant salt stress tolerance. Proceedings of the National Academy of Sciences of the United States of America, 118(46), e2107417118.

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Comprehensive Characterization of Catalysts

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The morphologies of samples were characterized via TEM (Technai G20 S-TWIN, FEI, Hillsboro, OR, USA) with a 200 kV acceleration voltage. The element distribution was studied using HAADF-STEM-EDX mapping. The phase of catalysts was analyzed using XRD (D8, Brook, Nordrhein-Westfalen, Germany) with Cu Kα radiation (λ = 0.15418 nm). The chemical states of surface elements were characterized via X-ray photoelectron spectroscopy (Thermo Fisher Scientific K-Alpha, ESCALAB 250XI, Waltham, MA, USA). Thermogravimetric analysis (STA, 2500) for the carbon content was conducted from 30 °C to 800 °C under an O₂ atmosphere. The content of each element in the sample was analyzed by inductively coupled plasma atomic emission spectrometry (ICP-AES, Agilent 5110, Santa Clara, CA, USA).

Xiang L., Hu Y., Zhao Y., Cao S, & Kuai L. (2023). Carbon-Supported High-Loading Sub-4 nm PtCo Alloy Electrocatalysts for Superior Oxygen Reduction Reaction. Nanomaterials, 13(16), 2367.

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Cultivation and Zinc Treatment of P. aeruginosa

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PAO1 was purchased from American Type Culture Collection (#15692, ATCC; Manassas, VA, USA). P. aeruginosa containing a triple hemagglutinin tag on its czcR N-terminus (CzcR-HA PAO1) was kindly provided by Dr. Karl Perron (University of Geneva; Geneva, Switzerland) [41 (link)]. P. aeruginosa was cultured in LB (Alfa Aesar, #3339936; Haverhill, MA, USA) overnight at 200 rpm and 37 °C, then diluted to 5 × 10⁷ CFU/mL in LB (as a control) or LB containing zinc (800 µM, as ZnSO₄, except for growth curve as indicated), and incubated for 16 h at 37 °C (shaking at 200 rpm). Zinc concentration was confirmed using Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES; Agilent 5110; Agilent Technologies, Santa Clara, CA, USA) [66 (link)].

Wu T., Gagnon A., McGourty K., DosSantos R., Chanetsa L., Zhang B., Bello D, & Kelleher S.L. (2021). Zinc Exposure Promotes Commensal-to-Pathogen Transition in Pseudomonas aeruginosa Leading to Mucosal Inflammation and Illness in Mice. International Journal of Molecular Sciences, 22(24), 13321.

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Quantifying Microbial Impact on Fluid Chemistry

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To assess the impact of microbes on the fluid chemistry, inductively coupled plasma-optical emission spectroscopy (ICP–OES) was employed using an Agilent 5110 (Agilent, Milton Keynes, UK) at the Open University, as previously described [47 (link)]. Fluid samples were taken at the end of the growth experiments from both the biotic and abiotic test groups and filtered using 0.22 μm filters (Starlab, Milton Keynes, UK) and acidified through the addition of 1% nitric acid (Sigma-Aldrich, Gillingham, UK) prior to analysis.

Kelbrick M., Oliver J.A., Ramkissoon N.K., Dugdale A., Stephens B.P., Kucukkilic-Stephens E., Schwenzer S.P., Antunes A, & Macey M.C. (2021). Microbes from Brine Systems with Fluctuating Salinity Can Thrive under Simulated Martian Chemical Conditions. Life, 12(1), 12.

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Degradation Analysis of Mg-Zn-Y-Nd Alloy

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The surface morphology of the leached samples before and after removal of the degradation products was observed by scanning electron microscopy (FEI Quanta 200, Eindhoven, Holland), and the elemental composition of each surface was also detected using the scanning electron microscopy matching energy dispersion spectrometer.26 (link) To investigate the mechanical property of the degraded Mg-Zn-Y-Nd alloy, surface hardness was detected as reported.27 According to American Society for Testing Materials G1-03, the degradation products of surface deposition were removed by chromic acid solution for Mg alloy samples. The inductively coupled plasma optical emission spectrometry (ICP-OES, Agilent 5110, Palo Alto, CA, USA) was used to measure the concentration of Mg²⁺, Zn²⁺, Y³⁺, Nd³⁺ in the extract and culture medium.

Wang S., Zhu S.J., Zhang X.Q., Li J.A, & Guan S.K. (2019). Effects of degradation products of biomedical magnesium alloys on nitric oxide release from vascular endothelial cells. Medical Gas Research, 9(3), 153-159.

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