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Pettrace 860 cyclotron

Manufactured by GE Healthcare
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Sourced in United States, Sweden

The PETtrace 860 cyclotron is a particle accelerator designed for the production of medical radioisotopes used in Positron Emission Tomography (PET) imaging. It generates high-energy proton beams that can be used to produce a variety of PET radioisotopes, such as fluorine-18, carbon-11, and nitrogen-13, which are commonly used in PET imaging procedures.

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Lab products found in correlation

Crc 55tpet dose calibrator, by Mirion Technologies (4 mentions) Sep pak c18 light cartridge, by Waters Corporation (3 mentions) H asp otbu h novasyn tg resin, by Merck Group (3 mentions) Sep pak light 46 mg accell plus qma carbonate cartridges, by Waters Corporation (3 mentions) P scn bn nota, by Macrocyclics (1 mentions)

Close spelling variants of this product

PETtrace cyclotron (87 citations) PETtrace 860 (2 citations) PETtrace (21 citations)

6 protocols using pettrace 860 cyclotron

1

Radiolabeled Tracer Synthesis

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All reagents were purchased from commercial suppliers and used without further purification. H-Asp (OtBu)-H NovaSyn TG resin (0.21 mmol/g) was obtained from Merck (Singapore). Fmoc-amino acids, HATU and HOAt, were obtained from Advanced Chemtech (Sabah, Malaysia). Fmoc-glutamic acid was t-butyl protected. (p-SCN-Bn)-NOTA was purchased from Macrocyclics (Plano, TX, USA). Sep-Pak® light (46 mg) Accell™ plus QMA carbonate cartridges and Sep-Pak® C18 light cartridges were purchased from Waters Corporation (Milford, CT, USA). Saline solution (0.9% w/v) was purchased from Braun Medical Industries (Singapore). All other chemicals and reagents were purchased from Sigma-Aldrich (Singapore), Fisher Scientific (Waltham, MA, USA) and Tokyo Chemical Industry (Chuo-ku, Tokyo, Japan). Carrier free aqueous [18F] fluoride was produced via the [18O(p,n)18F] nuclear reaction (GE PETtrace 860 cyclotron, Boston, MA, USA). Quality control of radiolabelled tracer was performed on a UFLC Shimadzu HPLC (Kyoto, Japan) system equipped with a dual-wavelength UV detector and a PMT-radio detector (Flow-Ram, Lab Logic, Broomhill, Sheffield, UK). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, Florham Park, NJ, USA).
Hartimath S.V., Ramasamy B., Xuan T.Y., Rong T.J., Khanapur S., Cheng P., Hwang Y.Y., Robins E.G, & Goggi J.L. (2022). Granzyme B PET Imaging in Response to In Situ Vaccine Therapy Combined with αPD1 in a Murine Colon Cancer Model. Pharmaceutics, 14(1), 150.
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2

Radiosynthesis and Characterization of [18F]AlF-mNOTA-GZP

Cited 1 time
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The synthesis of NOTA–β-Ala-Gly-Gly-Ile-Glu-Phe-Asp-CHO (mNOTA-GZP) and characterisation details have been described previously [16 (link)]. The radiosynthesis of [18F]AlF-mNOTA-GZP has also been described previously [16 (link)]. No-carrier-added aqueous [18F]fluoride ion was produced via the [18O(p,n)18F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual-wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA). [18F]AlF-mNOTA-GZP was formulated as a colourless solution of 10% ethanol in saline (pH = 7.4) with a nondecay corrected radiochemical yield of 12-18%, a radiochemical purity of 99%, and molar activity of 50-81 GBq/μmol (n = 3) after a total reaction and purification time of 50 minutes.
Goggi J.L., Ramasamy B., Tan Y.X., Hartimath S.V., Tang J.R., Cheng P., Msallam R., Chacko A.M., Hwang Y.Y, & Robins E.G. (2021). Granzyme B PET Imaging Stratifies Immune Checkpoint Inhibitor Response in Hepatocellular Carcinoma. Molecular Imaging, 2021, 9305277.
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3

Radiolabeling of [11C]CO2 using Cyclotron

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[11C]CO2 was prepared by bombardment of 14N2 target gas containing 1% O2 with protons (10–60 µA, 16.5 MeV) using a PETtrace 860 cyclotron (GE Healthcare).
Wenz J., Arndt F, & Samnick S. (2022). A new concept for the production of 11C-labelled radiotracers. EJNMMI Radiopharmacy and Chemistry, 7, 6.
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4

Radiolabeling of [19F]siPSMA-14 Peptide

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Radiolabeling of the peptide [19F]siPSMA-14 (Technical University of Munich, Garching, Germany) was performed by isotope exchange (18F/19F). For this purpose, fluorine-18 was generated using a PETtrace 860 cyclotron (GE Healthcare, Uppsala, Sweden). Specific details of this new radiopharmaceutical are yet to be reported elsewhere. According to the manufacturer’s (Scintomics Molecular, ATT GmbH, Fürstenfeldbruck, Germany) synthesis instructions for the synthesis kit, the ligand was synthesized on a GRP cassette module. This routine synthesis achieves an average specific activity (end of synthesis) of AS = (105 ± 40) MBq/µg for [18F]siPSMA-14.
Löffler J., Herrmann H., Scheidhauer E., Wirth M., Wasserloos A., Solbach C., Glatting G., Beer A.J., Rasche V, & Winter G. (2022). Blocking Studies to Evaluate Receptor-Specific Radioligand Binding in the CAM Model by PET and MR Imaging. Cancers, 14(16), 3870.
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5

Radiolabeling of NOTA-conjugated peptides

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H-Asp(OtBu)-H NovaSyn TG resin (0.21 mmol/g) was obtained from Merck. Fmoc-amino acids, HATU and HOAt were obtained from Advanced Chemtech. Fmoc-glutamic acid was t-butyl protected. (p-SCN-Bn)-NOTA was purchased from Boc Sciences and Macrocyclics. Glacial acetic acid was purchased from JT Baker. Sep-Pak® light (46 mg) Accell™ plus QMA carbonate cartridges and Sep-Pak® C18 light cartridges were purchased from Waters Corporation. Saline solution (0.9% w/v) was purchased from Braun Medical Industries. All other chemicals and reagents were purchased from Sigma-Aldrich, Fisher Scientific, and Tokyo Chemical Industry. No-carrier-added aqueous [18F]fluoride ion was produced via the [18O(p,n)18F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual-wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA).
Goggi J.L., Hartimath S.V., Xuan T.Y., Khanapur S., Jieu B., Chin H.X., Ramasamy B., Cheng P., Rong T.J., Fong Y.F., Yuen T.Y., Msallam R., Chacko A.M., Renia L., Johannes C., Hwang Y.Y, & Robins E.G. (2021). Granzyme B PET Imaging of Combined Chemotherapy and Immune Checkpoint Inhibitor Therapy in Colon Cancer. Molecular Imaging and Biology, 23(5), 714-723.
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6

Radiolabeled NOTA-peptide Synthesis

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H-Asp(OtBu)-H NovaSyn TG resin (0.21 mmol/g) was obtained from Merck. Fmoc-amino acids, HATU and HOAt were obtained from Advanced Chemtech. Fmoc-glutamic acid was t-butyl protected. (p-SCN-Bn)-NOTA was purchased from Boc Sciences and Macrocyclics. Glacial acetic acid was purchased from JT Baker. Sep-Pak® light (46 mg) Accell™ plus QMA carbonate cartridges and Sep-Pak® C18 light cartridges were purchased from Waters Corporation. Saline solution (0.9 % w/v) was purchased from Braun Medical Industries. All other chemicals and reagents were purchased from Sigma-Aldrich, Fisher Scientific, and Tokyo Chemical Industry. No-carrier-added aqueous [18F]fluoride ion was produced via the [18O(p,n)18F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA).
Goggi J.L., Tan Y.X., Hartimath S.V., Jieu B., Hwang Y.Y., Jiang L., Boominathan R., Cheng P., Yuen T.Y., Chin H.X., Tang J.R., Larbi A., Chacko A.M., Renia L., Johannes C, & Robins E.G. (2020). Granzyme B PET Imaging of Immune Checkpoint Inhibitor Combinations in Colon Cancer Phenotypes. Molecular Imaging and Biology, 22(5), 1392-1402.
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