Hs 4800 pro system

Manufactured by Tecan

Sourced in Switzerland

The HS 4800 Pro system is a liquid handling instrument designed for automated sample processing in life science laboratories. It features a modular design, enabling customization to meet specific research needs. The system is capable of performing various liquid handling tasks, such as pipetting, dilution, and sample transfer, to support a range of applications in fields like drug discovery, genomics, and protein research.

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Lab products found in correlation

Genepix 4000b, by Molecular Devices (2 mentions) Ingenuity system, by Qiagen (1 mentions) Mircury lna microrna array, by Qiagen (1 mentions)

2 protocols using hs 4800 pro system

miRNA Expression Profiling Protocol

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miRNA expression was analyzed using specific arrays (Exiqon), following the manufacturer’s recommendations. Briefly, fluorescent reagents were reconstituted, and cDNAs were synthesized and labeled. Subsequently, the samples were heated at 95 °C in the dark, hybridized, and washed with the robotic HS 4800 Pro system (Tecan^®, Männedorf, Switzerland). Fluorescence was scanned using a GenePix 4000 B microarray scanner (Molecular Devices, San Jose, CA, USA). Image processing was performed using Gene Prix Pro (v. 6.0), and data processing was performed using MEV (MultiExperiment Viewer) and EXpander 6.0. Functional enrichment analysis of deregulated genes, analysis of canonical pathways, correlation networks, and gene–gene and gene–miRNA interactions were defined using the Ingenuity Pathway Analysis (IPA) software (Ingenuity Systems; Qiagen China Co., Ltd., Shanghai, China) and the Database for Annotation, Visualization, and Integrated Discovery (DAVID) functional annotation.

Fernández-Regueras M., Carbonell C., Salete-Granado D., García J.L., Gragera M., Pérez-Nieto M.Á., Morán-Plata F.J., Mayado A., Torres J.L., Corchete L.A., Usategui-Martín R., Bueno-Martínez E., Rojas-Pirela M., Sabio G., González-Sarmiento R., Orfao A., Laso F.J., Almeida J, & Marcos M. (2023). Predominantly Pro-Inflammatory Phenotype with Mixed M1/M2 Polarization of Peripheral Blood Classical Monocytes and Monocyte-Derived Macrophages among Patients with Excessive Ethanol Intake. Antioxidants, 12(9), 1708.

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Comprehensive miRNA Expression Analysis

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MiRNA expression was analysed with specific arrays miRCURY LNA microRNA Array, v.10.0 (Exiqon), following the manufacturer’s recommendations. Then, fluorescent reagents were reconstituted and cDNAs were synthesized and labelled. Following this, samples were heated at 95° C in darkness, hybridized, and washed with the robotic HS 4800 Pro system (Tecan®). Fluorescence was scanned with a GenePix 4000B (Axon Instruments™) microarray scanner. Image processing was accomplished with the Gene Prix Pro (v 6.0). The data for the analysis were generated from a single channel following manufacturer’s instructions. Raw data processing was performed with the ExiMiR package from R ^{28 , 29}. Quantile normalization was performed using the Robust Microarray Analysis (RMA) algorithm^{30 (link)} from the BioConductor (http://www.bioconductor.org/) tools suite. All expression data were deposited in the GEO database (GEO: GSE71923).

Cañueto J., Cardeñoso-Álvarez E., García-Hernández J.L., Galindo-Villardón P., Vicente-Galindo P., Vicente-Villardón J.L., Alonso-López D., De Las Rivas J., Valero J., Moyano-Sanz E., Fernández-López E., Mao J.H., Castellanos-Martín A., Román-Curto C, & Pérez-Losada J. (2017). miR-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma. The British journal of dermatology, 177(1), 168-178.

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