Uas gcamp3

Manufactured by Bloomington Drosophila Stock Center

Sourced in Canada

UAS-GCaMP3 is a genetically encoded calcium indicator that can be used to monitor neuronal activity in Drosophila. It consists of the calcium-binding protein calmodulin fused to a circularly permuted green fluorescent protein (GFP). Upon binding of calcium, the fluorescence of the GFP is increased, allowing for the visualization of calcium dynamics in live cells.

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4 protocols using uas gcamp3

Genetic Tools for Drosophila Gustatory Receptors

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Flies were raised on standard cornmeal fly food at 25°C and 70% relative humidity. The following fly lines were used: Gr5a-GAL4, Gr43a-GAL4, Gr64a-GAL4, Gr61a-GAL4, Gr64c-GAL4, Gr64d-GAL4, and Gr64e-GAL4 (ref^{16 (link)}); Gr64f^LexA and Gr43a^GAL4 (ref^{13 (link)}); UAS-GCaMP3 (ref⁴³); UAS-KIR2.1 (ref^{37 (link)}); UAS-TdTomato and UAS-GFP (Bloomington stock center); poxn^Δ^M22-B5 (ref^{44 (link)}); poxn⁷⁰ (ref ^{28 (link)}).

LeDue E.E., Chen Y.C., Jung A.Y., Dahanukar A, & Gordon M.D. (2015). Pharyngeal sense organs drive robust sugar consumption in Drosophila: Pharyngeal taste in adult Drosophila. Nature communications, 6, 6667.

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Live Imaging of Drosophila Cell Junctions and Dynamics

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The following markers were used for live imaging: endo-DE-cadherin:GFP (Huang et al., 2009 (link)), ubi-DE-cadherin:GFP (Oda and Tsukita, 2001 (link)), UAS-DE-cadherin:GFP (gift of N. Gorfinkiel, Consejo Superior de Investigaciones Cientificas–Universidad Autónoma de Madrid, Madrid, Spain), UAS-p120-catenin:GFP (Bloomington Drosophila Stock Center; Myster et al., 2003 (link)), UAS-actin:RFP (Simões et al., 2006 (link); gift of N. Gorfinkiel), sqh-GFP:moesin (Kiehart et al., 2000 (link)), UAS-mCherry:moesin (Millard and Martin, 2008 (link)), sqh-sqh:GFP (Royou et al., 2004 (link)), sqh-sqh:mCherry (Martin et al., 2009 (link)), sqh-GFP:rok^K116A (Simões et al., 2010 (link)), UAS-GFP:clc (Chang et al., 2002 (link)), 20XUAS-shi^ts1:GFP (Pfeiffer et al., 2012 (link)), UAS-Apoliner (Bloomington Drosophila Stock Center; Bardet et al., 2008 (link)), UAS-GFP (gift of U. Tepass, University of Toronto, Toronto, Canada), 10XUAS-myr:GFP (Bloomington Drosophila Stock Center; Pfeiffer et al., 2010 (link)), UAS-GCaMP3 (Bloomington Drosophila Stock Center; Tian et al., 2009 (link)), and UAS-arf6:GFP (this study). daughterless-Gal4 or tubulin-Gal4 (Bloomington Drosophila Stock Center) were used to drive ubiquitous expression of all UAS transgenes. tubulin-Gal4 was used for all overexpression experiments. yellow white flies were used for controls.

Hunter M.V., Lee D.M., Harris T.J, & Fernandez-Gonzalez R. (2015). Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair. The Journal of Cell Biology, 210(5), 801-816.

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Genetic Tools for Drosophila Gustatory Receptors

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Drosophila neuronal and muscular reporters

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Following lines were used: OK371-Gal4 (genotype w¹¹¹⁸;OK371-Gal4;+, Bloomington #26160) for targeting glutamatergic neurons, MHC-tauGFP [22 (link)] as a muscle reporter line, MT11-Gal4 (genotype w;+;P{GawB}MT11)(Bloomington #37295), UAS-mCD8::mRFP (genotype y,w;UAS-mCD8::mRFP;+, Bloomington #27398 and y,w;+;UAS-mCD8::mRFP) (Bloomington #27399 (3^rd Chr.)),10xUAS-mCD8::GFP(genotype w;+;10xUAS-mCD8::GFP)(Bloomington #32184) UAS-TrpA1 (genotype w;UAS-TrpA1;+) (Bloomington #26263), UAS-shi^TS (w;+;UAS-shi^TS [39 (link)]), UAS-ChR2(H134R) (w;UAS-ChR2(H134R);+, Bloomington #28995), UAS-eNpHR (w;+;UAS-eNpHR::YFP, Bloomington #41752 gift from Leslie Griffith), UAS-PaGFP(A206K) (w;+;UAS-PaGFP(A206K), gift from A.S. Chiang [40 ]), UAS-GCamP3 (genotype w;+;UAS-GCamP3, Bloomington #32236), UAS-GCamP6s (genotype w¹¹¹⁸;UAS-GCamP6s;+, Bloomington #42746), OregonR (genotype +/w;+/UAS-TrpA1;+/+). For PaGFP experiments genotype was y,w/w;UAS-mCD8::mRFP; UAS-PaGFP(A206K).

Hückesfeld S., Schoofs A., Schlegel P., Miroschnikow A, & Pankratz M.J. (2015). Localization of Motor Neurons and Central Pattern Generators for Motor Patterns Underlying Feeding Behavior in Drosophila Larvae. PLoS ONE, 10(8), e0135011.

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