GSE-standardized preparation was a gift from Kikkoman Corp. (Nado City, Japan). The preparation composition is as follows: 89.3% procyanidins, 6.6% monomeric flavanols, 2.24% moisture content, 1.06% of protein, and 0.8% of ash, as reported recently (Derry et al., 2013 (link), Velmurugan et al, 2010a (link), 2010b (link)). Purchased antibodies include anti-CDX2, anti-CK20, anti-Surfactant D and anti-TTF-1 (all from Abcam). Anti-mouse and anti-rabbit horseradish peroxidase (HRP) secondary antibodies were from Invitrogen (Carlsblad, CA) and Cell Signaling Technology (Beverly, MA). RNA was isolated via the Qiagen RNeasy Kit, amplified via the Qiagen RT2 RNA qPCR kit. Additionally, RNA transcript was quantified via specific RNA TaqMan primers for Cdx2 from Life technologies (Grand Island, NY) and primers for mouse Ck20 from Invitrogen.
Anti ck20
Manufactured by Abcam
Anti-CK20 is a primary antibody that detects the CK20 protein. CK20 is a member of the cytokeratin family and is expressed in a variety of epithelial cells.
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Lab products found in correlation
2 protocols using anti ck20
1
Standardized Preparation Analysis and Characterization
2
Evaluation of Cancer Marker Antibodies
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The primary antibodies used for cancer markers are as follows: anti-CK18 anti-CK7, anti-TTF-1, anti-CK20, anti-PSA, anti-PSMA (Abcam), and anti-CDX2 (Cell Marque), The secondary antibody was Alexa Fluor-647 conjugate goat anti-rabbit IgG (Invitrogen). The staining protocol for primary and secondary antibodies is summarized in SI. To evaluate the staining efficiency of each marker on respective cell lines, the buffer/blood containing cancer cell lines that were pre-stained with CellTracker were captured, purified and released on the membrane chip, followed by staining with respective antibody pair (see SI). The staining efficiency is defined as: (Number of Alexa Flour-647 positive cells) / (Number of CellTracker green-positive cells) × 100%.
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