Log In Sign up
The largest database of trusted experimental protocols

Anti mouse cy5

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Anti-mouse Cy5 is a fluorescent labeling reagent used to detect mouse proteins in various applications, such as Western blotting, immunohistochemistry, and flow cytometry. It contains the cyanine-based Cy5 dye, which emits light in the red/far-red region of the visible spectrum when excited.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Ventana discovery xt, by Roche (1 mentions) X0909, by Agilent Technologies (1 mentions) Formalin, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Mouse anti ha, by Proteintech (1 mentions) Chicken anti map2, by Abcam (1 mentions) Mouse anti parkin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti tdp 43, by Proteintech (1 mentions) Mouse anti v5, by Proteintech (1 mentions) Rabbit anti pink1, by Novus Biologicals (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti gapdh, by Proteintech (1 mentions) Anti rabbit cy3, by Thermo Fisher Scientific (1 mentions) Anti h3k9me3, by Active Motif (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Streptavidin hrp, by Vector Laboratories (1 mentions) Dab kit, by Vector Laboratories (1 mentions)

Spelling variants of this product

PE–Cy5–anti-mouse Ly-6 A/E (9 citations) Anti-mouse CD45 PerCP-Cy5.5 (4 citations) Cy5-conjugated anti-mouse secondary antibody (4 citations) Cy5.5-conjugated anti-mouse CD8 mAb (2 citations) PE)-Cy5 anti-mouse Ly-6A/E (Sca-1) (clone D7; (2 citations) Anti-mouse CD4-PerCP-Cy5.5 (2 citations) PerCP-Cy5.5-conjugated anti-mouse CD11b (M1/70, (2 citations) PerCP-Cy5.5 anti-mouse IL-17A (eBio17B7, (2 citations) Percp-Cy5.5-conjugated anti-mouse F4/80 (2 citations) Cy5 anti-mouse CD3 antibody (1 citations)

5 protocols using anti mouse cy5

1

Immunohistochemical Analysis of γ-H2AX in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were harvested 2 weeks after the beginning of treatments and were formalin-fixed and paraffin-embedded. Four micrometer thick paraffin-embedded tissue sections were cut and immunostained using an automated Ventana Discovery XT staining system (Ventana Medical Systems). Antigen retrieval was performed in cell conditioner 1 and slides were incubated with anti-phospho-histone γ-H2AX antibody (1:250 dilutions in PBS at 37 °C for 60 min, clone JBW301, EMD Millipore, Temecula, CA) on automatic. Then, on the bench, slides were incubated for 20 min with blocking solution (Dako, Agilent, #X0909) followed by incubation with the secondary antibody (1:250 dilution in PBS at RT for 45 min; anti-mouse Cy5, Life Technologies Inc., #A10524). Finally, slides were incubated 15 min at RT with a 0.1% (w/v) solution of Sudan Black in 70% ethanol to quench tissue auto fluorescence. Slides were mounted using ProLong® Gold Antifade Mountant with DAPI (Life Technologies Inc., P-36931). Between each step, except after the blocking steps, slides were washed twice with PBS. Slides were stored at 4 °C and scanned the next day. A negative control slide was done in parallel where PBS replaced the primary antibody.
Fleury H., Malaquin N., Tu V., Gilbert S., Martinez A., Olivier M.A., Sauriol A., Communal L., Leclerc-Desaulniers K., Carmona E., Provencher D., Mes-Masson A.M, & Rodier F. (2019). Exploiting interconnected synthetic lethal interactions between PARP inhibition and cancer cell reversible senescence. Nature Communications, 10, 2556.
+ Open protocol
+ Expand
2

Immunofluorescent Detection of HCV NS3

Check if the same lab product or an alternative is used in the 5 most similar protocols
To detect the expression NS3, wild type DC2.4 and NS3 DC2.4 cells were fixed with 4% formalin (Sigma Aldrich) for 20 min, permeabilized with methanol at -20°C then blocked in 2.5% BSA (Sigma Aldrich) in PBS. The primary antibody, mouse anti-HCV NS3 gt1b (Virostat) diluted in 1% BSA, 0.3% Triton X-100 (Sigma Aldrich) in PBS, was added and incubated overnight at 4°C. After washing, the cells were then incubated for 1 h with fluorophore-conjugated secondary antibody (1:300) anti-mouse – Cy5 (Life Technologies). Cells were visualized by fluorescent microscopy (Zeiss LSM-700).
Mekonnen Z.A., Masavuli M.G., Yu W., Gummow J., Whelan D.M., Al-Delfi Z., Torresi J., Gowans E.J, & Grubor-Bauk B. (2020). Enhanced T Cell Responses Induced by a Necrotic Dendritic Cell Vaccine, Expressing HCV NS3. Frontiers in Microbiology, 11, 559105.
+ Open protocol
+ Expand
3

Antibody Validation for Western Blot, IP, and IF

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for western blotting, immunoprecipitation, and immunofluorescence assays: mouse anti-FLAG (Sigma-Aldrich, F3165); mouse anti-HA (Proteintech, 66006-1); rabbit anti-HA (CST, 3724); rabbit anti-TDP-43 (Proteintech, 10782-2-AP); rabbit anti-PINK1 (Novus Biologicals, BC100-494); mouse anti-Parkin (Santa Cruz, sc-32282); mouse anti-V5 (Proteintech, 66007-1); mouse anti-GAPDH (Proteintech, 60004-1); rabbit anti-Tubulin (MBL, PM054); mouse anti-Lamin C (DSHB, LC28.26); and chicken anti-MAP2 (Abcam, ab5392). Horseradish peroxidase-conjugated secondary antibodies: anti-mouse (Sigma-Aldrich, A4416) and anti-rabbit (Sigma-Aldrich, A9169). Fluorescent secondary antibodies: anti-mouse Cy5 (Life Technologies, A10524), anti-rabbit Alexa Fluor® 488 (Life Technologies, A11012), and anti-chicken Alexa Fluor® 633 (Sigma-Aldrich, A-21103).
Sun X., Duan Y., Qin C., Li J.C., Duan G., Deng X., Ni J., Cao X., Xiang K., Tian K., Chen C.H., Li A, & Fang Y. (2018). Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy. Cell Death & Disease, 9(10), 953.
+ Open protocol
+ Expand
4

Visualizing Histone Modifications in Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
To visualize H3K9me3 and H4K20me1, we stained fixed embryos with primary antibodies (rabbit anti-H3K9me3 and mouse anti-H4K20me1, Active Motif Inc.) diluted at 1:500 in 1× PBT overnight at 4°C on a platform rocker. Embryos were then washed three times at 10 min each with 1× PBT and then stained with fluorescently conjugated secondary antibodies at room temperature for 1 hour on a platform rocker in the dark. Secondary antibodies used in this study were anti-rabbit Cy3 and anti-mouse Cy5 (both at 1:300; Invitrogen, Thermo Fisher Scientific Inc., USA). Embryos were then washed as stated above and then mounted on a slide with VECTASHIELD mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories Inc., USA).
Dalla Benetta E., Antoshechkin I., Yang T., Nguyen H.Q., Ferree P.M, & Akbari O.S. (2020). Genome elimination mediated by gene expression from a selfish chromosome. Science Advances, 6(14), eaaz9808.
+ Open protocol
+ Expand
5

Visualization of Hyaluronan and Staphylococcal Toxin in Tumor Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to incubation with bHs-ST in vitro, sections of PANC-1 tumors were de-paraffinized and rehydrated. Uninduced and induced χ8768-bHs (108 CFU), PBS or bovine hyaluronidase (Sigma) were incubated on tissue sections overnight in a humidified 37°C incubator. Following treatment, specimens were incubated with a biotinylated HA binding protein (HABP, Sigma) at 5 μg/mL final concentration for 2 hours at 37°C. Slides were then washed, incubated with streptavidin-HRP at RT for 1 hr and visualized with a DAB kit (Vectastain). H&E and Masson’s Tricrhome were used for staining. PANC-1 tumor sections, skin and decalcified joints from NSG mice treated intravenously with χ8768-bHs (uninduced and induced). Sections were de-paraffinized and rehydrated and stained overnight with 2.5 μg/mL HABP, 1:100 anti-ST antibody (Santa Cruz, sc-52223), 1:100 anti-pan-cytokeratin (AE1/AE3) or according to H&E and Masson’s Trichrome protocols used by the Pathology Research Services Core (City of Hope). Streptavidin-PE (Vector), anti-mouse-Cy5 (Invitrogen), or anti-mouse-HRP 1:250 were then used to visualize HA, ST or pan-cytokeratin by brightfield or fluorescence microscopy (Zeiss Observer II), in addition to DAPI for visualizing nuclei during fluorescence imaging. Tiling was performed at 5X or 10X, while higher resolution images for ST, HA and DAPI were done at 100X (oil).
Ebelt N.D., Zuniga E., Passi K.B., Sobocinski L.J, & Manuel E.R. (2019). Hyaluronidase-Expressing Salmonella Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma. Molecular cancer therapeutics, 19(2), 706-716.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!