Vincristine

Manufactured by Cayman Chemical

Sourced in United States

Vincristine is a laboratory product manufactured by Cayman Chemical. It is a chemotherapeutic agent derived from the periwinkle plant, Catharanthus roseus. Vincristine is used in research settings for its ability to disrupt microtubule formation, which can lead to cell cycle arrest and apoptosis.

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Lab products found in correlation

Actinomycin d, by Cayman Chemical (3 mentions) Etoposide, by Thermo Fisher Scientific (3 mentions) Doxorubicin, by Merck Group (2 mentions) Ko143, by Bio-Techne (2 mentions) Chla 01r, by American Type Culture Collection (1 mentions) Chla 01, by American Type Culture Collection (1 mentions) Z ietd fmk, by R&D Systems (1 mentions) Actinomycin d, by Thermo Fisher Scientific (1 mentions) Z lehd fmk, by R&D Systems (1 mentions) Fmk008, by R&D Systems (1 mentions) Fmk007, by R&D Systems (1 mentions) 5 aza 2 deoxycytidine, by Merck Group (1 mentions) Alx 260 020 m001, by Enzo Life Sciences (1 mentions) Z vad fmk, by Enzo Life Sciences (1 mentions) Etoposide, by Cayman Chemical (1 mentions) Doxorubicin, by Cayman Chemical (1 mentions) Protame, by R&D Systems (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Cytarabine, by Merck Group (1 mentions) Chz868, by Novartis (1 mentions)

11 protocols using vincristine

Culture and Treatment of Group 4 MB Cells

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The Group 4 MB cell lines CHLA-01 and CHLA-01R, which derive from the primary tumor and a metastatic site, respectively, were purchased from the American Type Culture Collection (ATCC^® CRL3034^TM). Cells were cultured in suspension in DMEM:F12 Medium with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B27 Supplement to a final concentration of 2% (v/v), in a humidified atmosphere containing 5% CO₂ at 37 °C.
Luteolin and Dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich, St. Louis, MO, USA (Cat# L9283; Cat# D8418). Vincristine was obtained from Cayman Chemical Company, Ann Harbor, MI, USA (Cat# 11764).

Baroni M., Guardia G.D., Lei X., Kosti A., Qiao M., Landry T., Mau K., Galante P.A, & Penalva L.O. (2021). The RNA-Binding Protein Musashi1 Regulates a Network of Cell Cycle Genes in Group 4 Medulloblastoma. Cells, 11(1), 56.

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Evaluating Caspase Inhibitors and Cytotoxic Agents

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Cells were seeded in triplicate in a 6-well plate with 30 × 10⁴ cells per well and allowed to recover for 24 h before drug treatment. Cells were treated with caspase-8 inhibitor (Z-IETD-FMK, FMK007, R&D Systems), caspase-9 inhibitor (Z-LEHD-FMK, FMK008, R&D Systems) or a pan-caspase inhibitor (Z-VAD-FMK, ALX-260-020-M001, Enzo Life Science) at 40 μM, 40 μM or 50 μM, respectively, for 18 h. Cells were treated with either 10 nM vincristine (Cayman Chemical) and incubated for 72 h, 10 nM actinomycin D (Cayman Chemical) for 48 h, 50 μM etoposide (Acros Organics) for 24 h or 0.5 µg/ml TRAIL (PHC1634,Gibco) for 12 h. For multidrug treatment, combinations of etoposide (50 μM) and vincristine (10 nM), or etoposide (50 μM) and actinomycin D (10 nM) were added to the cells and incubated for 24 h. Cell viability was determined using trypan blue staining, and cell number was counted in three different wells on blinded samples. For DNA methyltransferase inhibition assay, RH30 cells were treated with either DMSO (vehicle) or 5-aza-2′deoxycytidine (Sigma-Aldrich) at 30 µM and 60 µM for 48 h prior to RNA isolation. Culture medium supplemented with fresh drug was changed every 24 h. All assays were performed at least twice to confirm results.

Kazim N., Adhikari A., Oh T.J, & Davie J. (2020). The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma. Cell Death & Disease, 11(1), 67.

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Preparation of Anticancer Compound Stocks

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ProTAME was purchased from Boston Biochem (Cambridge, MA) as a 20 mM stock solution in DMSO, stored at −20°C and diluted in RPMI 1640 (Fisher Scientific, Loughborough, UK) immediately before use. Etoposide, doxorubicin and vincristine were purchased from Cayman Chemical (Cambridge, UK), reconstituted in DMSO and stored at −20°C.

Crawford L.J., Anderson G., Johnston C.K, & Irvine A.E. (2016). Identification of the APC/C co-factor FZR1 as a novel therapeutic target for multiple myeloma. Oncotarget, 7(43), 70481-70493.

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Evaluating Targeted Leukemia Therapies

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All continuous variables were compared using a two-sided t-test or one-way ANOVA, followed by Dunnett’s test or Tukey’s test for multiple comparisons, and error bars in all figures represent the SEM. Survival curves were compared using the log-rank test with GraphPad software. BSK805, BVB808, BBT594, and CHZ868 were synthesized by Novartis. Dexamethasone, cytarabine, and doxorubicin were purchased from Sigma-Aldrich. Vincristine was purchased from Cayman Chemical. Ruxolitinib was purchased from SelleckChem.

Wu S.C., Li L.S., Kopp N., Montero J., Chapuy B., Yoda A., Christie A.L., Liu H., Christodoulou A., van Bodegom D., van der Zwet J., Layer J.V., Tivey T., Lane A.A., Ryan J.A., Ng S.Y., DeAngelo D.J., Stone R.M., Steensma D., Wadleigh M., Harris M., Mandon E., Ebel N., Andraos R., Romanet V., Dölemeyer A., Sterker D., Zender M., Rodig S.J., Murakami M., Hofmann F., Kuo F., Eck M.J., Silverman L.B., Sallan S.E., Letai A., Baffert F., Vangrevelinghe E., Radimerski T., Gaul C, & Weinstock D.M. (2015). Activity of the type II JAK2 inhibitor CHZ868 in B-cell acute lymphoblastic leukemia. Cancer cell, 28(1), 29-41.

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Cytotoxicity Assessment of Anticancer Drugs

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Initially, 5×10³ of PC9-KI, A375, and MDAMB231 cells were seeded on 96 well plates. After 24 h, 0.1 % DMSO or 10⁻⁵ μM, 10⁻⁴ μM, 10⁻³μM, 10⁻²μM, 10⁻¹μM, 1 μM, or 10 μM of colchicine (WAKO), vincristine (Cayman), thapsigargin (WAKO), and ML-240 (Sigma Aldrich) were added. After 48h, 25 μL of Cell Titer Glo 2.0 (Promega) was added and incubated at 37°C for 30 min. Absorbance at 490 nm was measured using ARVO X3.

Uchida Y., Matsushima T., Kurimoto R., Chiba T., Inutani Y, & Asahara H. (2021). Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells. FEBS letters, 595(5), 563-576.

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Quantification of Indole and Bisindole Alkaloids

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Monoindole alkaloid standards, such as catharanthine, tabersonine, and vindoline, as well as other chemicals, such as ammonium acetate, triethylamine, driselase, and sucrose, were purchased from Sigma-Aldrich (Missouri, USA). Bisindole alkaloid standards like vinblastine and vincristine were purchased from Cayman Chemicals (Michigan, USA). Acetonitrile and methanol were all LCMS grade and purchased from Merck (Darmstadt, Germany). Water was treated in a Milli-Q (Millipore, USA) water purification system. The other chemicals used for growth and elicitation studies, such as Gamborg B5 medium, 2,4-dichlorophenoxyacetic acid (2,4-D), jasmonate (JA), and methyl jasmonate (MeJA), were purchased from Duchefa Biochemie (Haarlem, Netherlands). The dye to stain for cell viability, fluorescein diacetate (FDA), was purchased from Merck Chemicals, Germany; the fluorochrome for mitochondria, MitoTracker Red FM, was purchased from Thermo Fisher Scientific Inc., Waltham, MA, USA.

Raorane M.L., Manz C., Hildebrandt S., Mielke M., Thieme M., Keller J., Bunzel M, & Nick P. (2022). Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus. Protoplasma, 260(2), 349-369.

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High-Throughput Screening of Transporter Modulators

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Calcitriol, calcipotriol, MK571, epigallocatechin gallate (EGCG), hyperoside, LTC₄, meropenem, mesalamine, nitazoxanide, and vincristine were purchased from Cayman Chemical (Ann Arbor, MI). AMP, ATP, doxorubicin, estradiol 17-(β-D-glucuronide) (E₂17βG), mitoxantrone, poly-D-lysine, thiazolyl blue tetrazolium bromide (MTT), and verapamil were procured from Sigma-Aldrich (St. Louis, MO). Apigenin and Ko143 were purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Calcein-AM was obtained from Corning Life Sciences (Corning, NY). [6,7-³H]E₂17βG (49.9 Ci mmol⁻¹) and [14, 15, 19, 20-³H]LTC₄ (177.6 Ci mmol⁻¹) were purchased from PerkinElmer (Waltham, MA).

Tan K.W., Sampson A., Osa-Andrews B, & Iram S.H. (2018). Calcitriol and Calcipotriol Modulate Transport Activity of ABC Transporters and Exhibit Selective Cytotoxicity in MRP1-overexpressing Cells. Drug Metabolism and Disposition, 46(12), 1856-1866.

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Vincristine-Induced Whisker Tactile Deficits

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Vehicle (sterile phosphate-buffered saline) or vincristine (Cayman Chemical, Ann Arbor, Michigan) dissolved in vehicle was i.p. administered daily for 5 consecutive days, then 2 day breaks, and daily for 5 consecutive days again. The dose of vincristine was at 0.3 mg/kg each injection. Unless otherwise indicated, whisker tactile behavioral tests were performed once every 2 or 3 days for up to 21 days. The whisker tactile test was performed in a blinded manner in that one examiner conducted vincristine injections and animal grouping, and another examiner who did not know the grouping performed in vivo whisker tactile behavioral tests. To begin the tests, mice were placed in a cage and habituated for 10 min. During habituation and subsequent experiments, the testing room only had a red light on so that animals could not see the examiner and the tactile stimulation filament. After the habituation, right whisker hairs of animals were displaced by the tactile stimulation filament in a caudal to rostral direction. The whisker tactile test was repeated 20 times with an interval of 1 min between trials. A positive whisker tactile behavioral response was considered when the testing animal exhibited an avoidance reaction to the tactile stimulation.

Sonekatsu M., Kanno S., Yamada H, & Gu J.G. (2020). Selective impairment of slowly adapting type 1 mechanoreceptors in mice following vincristine treatment. Neuroscience letters, 738, 135355.

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Cytotoxicity Evaluation of Chemotherapeutics

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Cells were seeded in triplicate in a 6-well plate with 30×10⁴ cells per well. After recovery for 24 hours, the cells were treated with 10 nM vincristine for 72 hours (Cayman Chemical), 10 nM actinomycin D for 48 hours (Cayman Chemical), and with 50 μM etoposide for 24 hours (Acros Organics) and with 0.5 mg/ml TRAIL (EMD Millipore) for 12 hours. For multidrug treatment, combination of etoposide (50 μM) and vincristine (10 nM), or etoposide (50 μM) and actinomycin D (10 nM) were added to the cells and incubated for 24 hours. Cell viability was determined using trypan blue staining, and cell number was counted in triplicate. All assays were performed at least three times to confirm results.

Mohamad T., Kazim N., Adhikari A, & Davie J.K. (2018). EGR1 interacts with TBX2 and functions as a tumor suppressor in rhabdomyosarcoma. Oncotarget, 9(26), 18084-18098.

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Culturing NALM6 and RS4;11 BCP-ALL Cells

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NALM6 cells and the RS4;11 TP53 wild-type BCP-ALL cell line were obtained from the JCRB Cell Bank (Osaka, Japan) and the American Type Culture Collection (Manassas, VA, USA), respectively. These cells were cultured in RPMI-1640 (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) containing 10% heat-inactivated FBS (Thermo Fisher, Waltham, MA, USA), in a humidified incubator at 37 °C under 5% CO₂.
The following chemicals were purchased: actinomycin D, daunorubicin, dimethyl sulfoxide (DMSO), methotrexate, prednisolone, vincristine, cytarabine, L-asparaginase, 6-mercaptopurine, and HDM201 (Cayman Chemical, Ann Arbor, MI, USA; Fujifilm Wako Pure Chemical Corporation; MedChemExpress, Monmouth Junction, NJ, USA; ProSpec, Ness-Ziona, Israel; TCI, Tokyo, Japan).

Nakagawa S., Kawahara K., Okamoto Y., Kodama Y., Nishikawa T., Kawano Y, & Furukawa T. (2022). Association between Dysfunction of the Nucleolar Stress Response and Multidrug Resistance in Pediatric Acute Lymphoblastic Leukemia. Cancers, 14(20), 5127.

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