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2 protocols using primary antibody for gapdh

1

Investigating Autophagy Regulators in Cells

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Primary antibody for GAPDH was from Bioworld (Minneapolis, MN), for LC3, p62, Vps34, Beclin-1 and Bcl-2 from Cell signaling (Beverly, MA. Wortmannin (WM) was purchased from sigma Aldrich (St Louis, MO). The supersignal west pico chemiluminescent substrate was obtained from Pierce (Rockford, IL).
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2

Cistanche deserticola Polysaccharide and L-dopa Interactions

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Cistanche deserticola polysaccharide (CDP) and l‐dopa were purchased from Yuanye Biotec (purity ≥ 98%; Shanghai, China). Hydrogen peroxide (H2O2), dimethyl sulphoxide (DMSO), NaOH, Triton X‐100, 4,5‐dimethylthiazol‐2‐yl‐2,5‐diphenyltetrazolium bromide (MTT) and an Annexin V‐FITC Apoptosis Detection Kit were purchased from Sigma‐Aldrich. 4% neutral paraformaldehyde was purchased from Biosharp (Hefei, China); and an Immunofluorescence Staining Kit (Alexa Fluor 488) and 2,7‐dichlorofluorescein‐diacetate (DCFH‐DA) were purchased from Beyotime Biotec (Shanghai, China). A Fontana‐Masson Stain Kit and a Nucleoplasmic Protein Extraction Kit were purchased from Sloarbio (Beijing, China). Human melanocyte growth supplement (HMGS), Dulbecco's modified Eagle medium (DMEM) and medium 254 were purchased from Gibco. Fetal bovine serum (FBS) was purchased from BI (Kibbutz Beit‐Haemek, Israel). Primary antibodies for β‐actin, TYR, TRP2, RAB27A, FSCN1, ERK, p‐ERK, JNK, p‐JNK, p38, p‐p38, NRF2 and HO‐1 were purchased from Cell Signaling Technology, primary antibody for MITF was purchased from St John's Laboratory, primary antibody for p‐MITF was purchased from Affinity Biosciences, primary antibody for GAPDH was purchased from Bioworld, and primary antibody for TRP1 was purchased from EMD Millipore.
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