Rabbit total igg sc 2027

Anti-YY1 (H414, sc-1703X) rabbit polyclonal antibody, anti-Myc (9E10) and anti-α-tubulin (sc-58666) monoclonal antibodies, rabbit total IgG (sc-2027) and mouse total IgG (sc-2025) were obtained from Santa Cruz Biotechnology (U.S.A.). Anti-Flag (M2)-agarose and anti-Flag M2 (F3165) monoclonal antibody were from Sigma (U.S.A.). Anti-p21 (10355-1-AP) was purchased from Proteintech^TM Group (China, Wuhan). Anti-p53-15P and anti-p53-33P rabbit polyclonal antibodies were gained from Dingguo Changsheng Biotechnology Co. LTD. (China, Beijing). Anti-INO80 (residue 1–526 aa), anti-hArp8, anti-hIes6, anti-hIes2, anti-p53, and anti-GAPDH rabbit polyclonal antibodies were raised against bacterially expressed proteins (Jilin University). Anti-p53 monoclonal antibody was from ZYMED (13–4000). Anti-pericentrin (ab4448) rabbit polyclonal antibody was from Abcam (UK).

Anti-Flag (M2) and anti-c-Myc agarose, as well as anti-Flag M2 (F3165) mouse monoclonal antibody were purchased from Sigma (St. Louis, MO, USA). Anti-BCCIP (16043-1-AP) and anti-p21 (10355-1-AP) polyclonal antibodies were from Proteintech Group (Wuhan, China). Anti-YY1 (H414) (sc-1703 or sc-1703X) rabbit polyclonal antibody, anti-Myc (9E10, sc-40) mouse monoclonal antibody, rabbit total IgG (sc-2027), and mouse total IgG (sc-2025) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-p53 mouse monoclonal antibody was provided by Boster Group (BM0101, Wuhan, China). The ChIP grade anti-p53 mouse monoclonal antibody was from Abcam (ab1101, USA). Anti-p53S15P (RLP0205), anti-p53T18P (RLT0212), anti-p53S20P (RLT0206), anti-GADD45 (RLT1832), anti-Bax (RLT0456), and anti-Flag (RLG0004) rabbit polyclonal antibodies, Bcl2 (RLM3041) and anti-HA (RLM3003) mouse monoclonal antibodies were obtained from Ruiying (Suzhou, China). Anti-GAPDH (NM_002046, full length) polyclonal antibody and anti-BCCIP (NM_078468, residues 1-322) mouse polyclonal antibody were raised against bacterially expressed proteins (Jilin University, Changchun, China). 5-Fluorouracil (5FU) (F6627, Sigma) was dissolved in dimethyl sulfoxide (DMSO) and prepared at a 500 mM concentration for storage. The final concentration of 5FU in cell culture medium was 250 μM.