Hippocampal sections from control postmortem human tissue were obtained from the University of Maryland repositories of the NIH NeuroBioBank. Antibodies used for western blotting were AIDA-1 C-10 (1:1000, Santa Cruz), tubulin (1:1000, Thermo Fisher), PSD95 (1:1000, NeuroMab), GAPDH (1:1000 Cell Signaling Tech), calnexin (1:1000, Genscript), Rab11 (1:1000, Cell Signaling Tech), Git1 (1:1000, Santa Cruz), Itsn1 (1:1000, Santa Cruz), AP2a1/2 (1:000, Santa Cruz), Asap1 (1:1000, Santa Cruz), and Srgap2 (1:1000, Proteintech). Antibodies for immunocytochemistry were Oct-4 (1:1000, Abcam), Sox-2 (1:500, Cell Signaling Tech), MAP-2 (1:1000, EnCor), GluN2B (1:500, Alomone), and GluN2A (1:500, Alomone). Mouse monoclonal antibodies 1A11 and 2B22 were developed and validated in house25 (link). Chicken AIDA-1 antibody 5707 was generated by Aves Labs to the peptide sequence GDRLHDDPPQKPPRC at the end of the second SAM domain of AIDA-1.
Glun2a
Manufactured by Alomone
GluN2A is a subunit of the NMDA receptor, a type of ionotropic glutamate receptor that is involved in synaptic transmission and plasticity in the central nervous system. The GluN2A subunit plays a crucial role in the functional and pharmacological properties of the NMDA receptor.
Automatically generated - may contain errors
Lab products found in correlation
Glun2b, by Alomone (2 mentions)
Tubulin, by Thermo Fisher Scientific (1 mentions)
Rab11, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Glun1, by BD (1 mentions)
α tubulin, by Merck Group (1 mentions)
Vibratome, by Leica camera (1 mentions)
Glun2b, by Merck Group (1 mentions)
4 protocols using glun2a
1
Characterizing Proteins in Human Hippocampus
2
Characterization of IgSF11 Antibodies
3
Antibody Characterization for Neuroscience Research
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The following antibodies were previously described: NGL-1 (#2040), NGL-2 (#2044; Um et al., 2018 (link)), NGL-3 (#1948; Lee et al., 2014 (link)), GluA1 (#1193), GluA2 (#1195; Kim et al., 2009 (link)). In addition, the following antibodies were purchased from commercial sources: GluN1 (Neuromab 75-272), GluN2A (Alomone AGC-003), GluN2B (Neuromab 75-101), PSD-95 (Neuromab 75-028), protein kinase Cα (PKCα; BD transduction 610108), CaMKIIα/β (Cell signaling 3362) and α-tubulin (Sigma T5168).
4
Immunohistochemical Analysis of Synaptic Proteins
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Mice were anaesthetized and perfused intracardiacally immediately after the acquisition of the trace fear conditioning, with 50 ml of ice‐cold 4% paraformaldehyde (PFA) in 0.1 M Na2HPO4/NaH2PO4 pH 7.4 buffer. Brains were post‐fixed overnight in 4% PFA at 4°C. Coronal sections (40 μm) were processed using a vibratome (Leica) and kept in a cryoprotective solution (30% glycerol, 30% ethylene glycol in PB 0.1 M pH 7.4) at −20°C until processing.
PLA was performed on floating sections using the Duolink in situ kit for brightfield detection (for main experiments) or fluorescent detection (for negative control experiments; Sigma) according to the manufacturer's instructions with the following modifications: Incubation with PLA probes was for 2 h at 37°C; ligation step was performed for 45 min at 37°C; amplification step was extended to 2 h at 37°C with a concentration of polymerase of 1/60. All antibodies were used at a concentration of 1/200 (GluN2B: Millipore 06‐600: GluN2A: alomone laboratories AGC‐002; PSD‐95: NeuroMab UC Davis 75‐028).
PLA was performed on floating sections using the Duolink in situ kit for brightfield detection (for main experiments) or fluorescent detection (for negative control experiments; Sigma) according to the manufacturer's instructions with the following modifications: Incubation with PLA probes was for 2 h at 37°C; ligation step was performed for 45 min at 37°C; amplification step was extended to 2 h at 37°C with a concentration of polymerase of 1/60. All antibodies were used at a concentration of 1/200 (GluN2B: Millipore 06‐600: GluN2A: alomone laboratories AGC‐002; PSD‐95: NeuroMab UC Davis 75‐028).
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