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Micro nikkor 60 mm lens

Manufactured by Nikon
Sourced in Japan

The Micro Nikkor 60-mm lens is a prime lens designed for close-up photography. It has a focal length of 60 millimeters and a maximum aperture of f/2.8. The lens is capable of 1:1 macro reproduction ratio, allowing for detailed, life-size captures of small subjects.

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5 protocols using micro nikkor 60 mm lens

1

Fossil Specimen Imaging and Analysis

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Fossils specimens were photographed using a digital camera (D3X-Nikon with Nikon Micro-Nikkor 60 mm lens) and measured (lengths, distances, angles) from high-resolution digital images by using Image J, a public domain processing program. Polished and lithological thin sections were made using standard methods and observed under binocular stereo-microscope (Leica MZ125 and Leica DM750P). Elemental maps were acquired using a Tornado M4 micro-XRF system (Bruker, Germany) equipped with a silicon drift detector and a Rh source operating at 50 kV and 600 µA. A spot size of 40 µm was employed with dwell times of 7 ms/pixel, and mapping was performed under vacuum. Image processing included spectral deconvolution and 3-pixel averaging. Tomographic images of ROMIP 57013 were obtained via the same methods and with the same machine as in Kouraiss et al.69 (link).
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2

Multimodal Imaging of Infantile Hemangiomas

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Non-clinician research staff conducted the procedures after subject acclimation to room conditions without draughts for least 15 minutes. Subjects were maintained in a calm state throughout. High-resolution digital color images were taken 30 cm perpendicular to the sites (Nikon D90 camera, Micro Nikkor 60-mm lens, Nikon R1 Wireless Close-Up flash, Nikon Inc., Nikon Corporation, Tokyo, Japan).45 (link) Three dimensional (3D) surface images were collected at 60 cm with an Artec MHT scanner (Artec Group, San Diego, CA, USA). Static IR images of IHs and controls were taken 60 cm perpendicular to the sites at an emissivity of 0.9816 (link) (FLIR T400 camera, range 7.5 – 13 μm; uncooled microbolometer focal plan array detector, 320 × 240 pixels resolution; sensitivity < 0.05°C at 30°C; accuracy ± 2°C (<2%), factory calibration, FLIR Systems, Inc., Wilsonville, OR, USA).
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3

Standardized Digital Skin Imaging

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Subjects acclimated to room conditions for at least 15 minutes and were maintained in a calm state. High resolution digital color images were collected at 30 cm perpendicular to the contralateral uninvolved skin site (Nikon D90 12.3 megapixels, Micro Nikkor 60-mm lens, cross polarization, and Nikon R1 Wireless Close-Up flash, Nikon Corporation, Tokyo, Japan) (23 (link)). Images were taken with standardized conditions (lighting, subject position), at a fixed distance, and with polarized light (24 (link)) and standardized for white balance and color (23 (link)). Images were color corrected (Macbeth color card), converted from RGB (to CIELab color space, and separated into L* (dark-light), a* (green-red) and b* (blue-yellow) channel images (ImageJ, NIH, Washington, DC, USA) (23 (link)). Mean lightness, red color and yellow color values were reported on 0 – 255 intensity scales.
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4

UV Photography for Floral Trait Analysis

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UV photography was conducted using a Baader U-filter. This filter transmits UV wavelengths from 325 to 369 nm, with maximal penetrance in the UV-A range at 360 nm (Savazzi, 2011 ). The filter was used with an AF Micro Nikkor 60 mm lens, several mounts, and a Nikon D70 DSLR digital camera. UV photographs were downloaded to Nikon Capture NX2 software and then transferred to Image J 1.46 for quantitative analyses. Image J is an open access processing and analysis program written in Java that can be downloaded from National Institutes of Health (http://rsbweb.nih.gov/ij). In Image J, a phenotypic trait, like petal surface area can be traced and digitized. The circumscribed area can be calculated and the resultant pixel measurement for the trait can be converted to a meaningful scale (e.g. mm2) based upon a reference scale used in the picture (here, a ruler). Multiple traits were measured including petal length, petal surface area, floral-head area, UV floral guide length, and proportion of petal surface area comprised of UV floral guide. All statistical tests were performed in SPSS.
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5

Macroscopic Specimen Videography

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Video recording of the live specimens was performed using a Nikon D500 and Micro NIKKOR 60 mm lens (Nikon) under red light (LED Lenser T2QC) with the following settings: ISO, 64000; f/2.8; and exposure, 1/60 s.
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