P tyramine

Manufactured by Merck Group

Sourced in United States

P-tyramine is a chemical compound that is commonly used as a laboratory reagent. It is a derivative of the amino acid tyrosine and has a basic chemical structure. P-tyramine serves as a core functional unit in various analytical and research applications within the scientific community.

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P-tyramine hydrochloride Tyramine

4 protocols using p tyramine

Radioactive Tracer Synthesis and Assay

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2-[1, 2-³H]-D-deoxyglucose, [7-¹⁴C]-benzylamine and the liquid scintillation cocktail Emulsifier-Safe were from Perkin Elmer SAS (Waltham, MA, USA). [¹⁴C]-tyramine was obtained from Sigma-Aldrich-Merck (Saint Quentin Fallavier, France). N-methyltyramine, p-tyramine, pargyline, synephrine, bovine albumin, collagenase and most of the other chemicals were from Sigma-Aldrich-Merck (Saint Quentin Fallavier, France). The indane hydrazino alcohol BTT 2052 was a generous gift from D. Smith and M. Salmi (Turku, Finland). The 2-benzofuranyl-2-imidazoline (BFI) was kindly given by Dr. A. Hudson (Univ. of Alberta, Edmonton, AB, Canada).

Carpéné C., Viana P., Fontaine J., Laurell H, & Grolleau J.L. (2022). Multiple Direct Effects of the Dietary Protoalkaloid N-Methyltyramine in Human Adipocytes. Nutrients, 14(15), 3118.

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Dopamine receptor agonist experiments

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DA and p-tyramine were from Sigma-Aldrich (St. Louis, MO, USA) and were prepared fresh on each day of experiments and dissolved directly in the recording buffer. (R)- and (S)-5-OH-DPAT (Axon MedChem BV, Groeningen, The Netherlands) were dissolved in DMSO at 10 mM and subsequently diluted into the recording buffer at the desired concentrations. Likewise, haloperidol (Abcam Chemicals, Cambridge, UK) was dissolved at 10 mM in DMSO and diluted in recording buffer to the final concentration. The maximum final concentration of DMSO used in any experiment was 0.1% v/v.

Ågren R., Stepniewski T.M., Zeberg H., Selent J, & Sahlholm K. (2021). Dopamine D2 Receptor Agonist Binding Kinetics—Role of a Conserved Serine Residue. International Journal of Molecular Sciences, 22(8), 4078.

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Pharmacological Evaluation of Adrenergic Agents

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(+)Noradrenaline-bitartrate, (±)p-synephrine, (±)p-octopamine hydrochloride, (±)p-tyramine, (+)-phenylephrine hydrochloride, prazosin hydrochloride, propranolol hydrochloride, EPPTB (RO-5212773, N-(3-Ethoxyphenyl)-4-(1-pyrrolidinyl)-3-(trifluoromethyl)benzamide) and Nω- nitro-L-arginine (L-NNA) were obtained from Sigma-Aldrich (Sydney, Australia). All drugs except RO-5212773 were dissolved in distilled water and then diluted with Krebs-bicarbonate solution. RO-5212773 was dissolved in 1% dimethyl sulfoxide (DMSO) prior to dilution with Krebs-bicarbonate solution.

Koh A.H., Chess-Williams R, & Lohning A.E. (2019). Differential mechanisms of action of the trace amines octopamine, synephrine and tyramine on the porcine coronary and mesenteric artery. Scientific Reports, 9, 10925.

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Inhibitory Activity of Compounds on MAOs

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The inhibitory activities of the compounds 3a-i and 3e₁-e₁₁ towards MAOs were assayed by the Amplex Red fluorescence method. The recombinant human MAOs (hMAO-A, M7316 and hMAO-B, M7441) and p-tyramine (T90344) were obtained from Sigma-Aldrich (St. Louis, MO, USA). hMAO-A and hMAO-B and Amplex Red assay kit were used to determine the production of H₂O₂ from substrate p-tyramine. The test compounds were dissolved in DMSO and then diluted to different concentrations with PBS buffer solution (DMSO <0.01%). 80 μL of hMAO-A or hMAO-B and 20 μL of different concentrations of compounds were added to a 96-well black microtiter plate, and then incubated for 15 min at 37°C in the dark. After this period, a substrate mixture was added quickly and the results were tested by a multi-detection microplate fluorescence reader with excitation/emission wavelengths of 544/590 nm. Data were shown as mean±SD of three independent experiments.

Guo J., Xu A., Cheng M., Wan Y., Wang R., Fang Y., Jin Y., Xie S.S, & Liu J. (2022). Design, Synthesis and Biological Evaluation of New 3,4-Dihydro-2(1H)-Quinolinone-Dithiocarbamate Derivatives as Multifunctional Agents for the Treatment of Alzheimer’s Disease. Drug Design, Development and Therapy, 16, 1495-1514.

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