Anti human cd8

Manufactured by Thermo Fisher Scientific

The Anti-human CD8 is a laboratory reagent used in flow cytometry applications. It is designed to specifically bind to the CD8 protein expressed on the surface of certain human T cells. This product can be used to identify and quantify CD8+ T cells in biological samples.

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Lab products found in correlation

Anti human cd3, by Thermo Fisher Scientific (2 mentions) Anti mouse cd4, by Thermo Fisher Scientific (2 mentions) Anti human cd25, by Thermo Fisher Scientific (1 mentions) Anti mouse cd3, by Thermo Fisher Scientific (1 mentions) Anti mouse cd8, by Thermo Fisher Scientific (1 mentions) Anti mouse pd 1, by Thermo Fisher Scientific (1 mentions) Anti mouse cd25, by Thermo Fisher Scientific (1 mentions) Facsaria 2 instrument, by BD (1 mentions) Celltrace violet cell proliferation kit, by Thermo Fisher Scientific (1 mentions) Canto 2 flow cytometer, by BD (1 mentions) Anti human cd3, by BD (1 mentions) Anti human cd8, by BD (1 mentions) Anti rna polymerase 2, by Abcam (1 mentions) Anti human cd4, by Thermo Fisher Scientific (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions) Anti mouse cd8, by BD (1 mentions) Anti mouse cd25, by BD (1 mentions) Anti mouse tcrβ, by BD (1 mentions) Anti mouse cd4, by BD (1 mentions) Anti mouse ifn γ, by BioLegend (1 mentions)

3 protocols using anti human cd8

Multiparameter Flow Cytometry Analysis

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Fluorescently conjugated antibodies were purchased from Ebioscience (anti-human CD3, anti-human CD8, anti-human PD1, anti-human CD25, anti-mouse CD3, anti-mouse CD8, anti-mouse PD1, anti-mouse CD25). Anti-human neuropilin-1 (FAB3870R) and anti-mouse neuropilin-1 (FAB566A) were purchased from R&D Systems. ITAg Tetramer - H-2 Kb OVA (SIINFEKL) conjugated to PE was purchased from MBL International Corporation, live/dead Fixable Viability Dye eFluor 450 and near infrared (Thermo Fisher). Celltrace violet cell proliferation kit was purchased from Thermo Fisher. Flow cytometric cell acquisition was done on a Canto II flow cytometer (BD Biosciences). Cell sorting was performed on a FACSAria II instrument (BD Biosciences). Flow cytometry data were analyzed using FlowJo software (TreeStar). Cells were gated on live cells (live/dead Fixable Viability Dye eFluor 450 negative).

Rossignol J., Belaid Z., Fouquet G., Guillem F., Rignault R., Milpied P., Renand A., Coman T., D’Aveni M., Dussiot M., Colin E., Levy J., Carvalho C., Goudin N., Cagnard N., Côté F., Babdor J., Bhukhai K., Polivka L., Bigorgne A.E., Halse H., Marabelle A., Mouraud S., Lepelletier Y., Maciel T.T., Rubio M.T., Heron D., Robert C., Girault I., Lebeherec D., Scoazec J.Y., Moura I., Condon L., Weimershaus M., Pages F., Davoust J., Gross D, & Hermine O. (2022). Neuropilin-1 cooperates with PD-1 in CD8+ T cells predicting outcomes in melanoma patients treated with anti-PD1. iScience, 25(6), 104353.

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Detailed Immunological Antibody Protocols

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The antibodies used in this study for flow cytometry include: anti-mouse CD8 (#563786), anti-mouse CD25 (#558642), anti-mouse TCRβ (#553174), anti-human CD3 (#563798), anti-human CD8 (#563795), and anti-human TCRαβ (#563826) from BD Biosciences; anti-mouse CD4 (#100544), anti-mouse IFN-γ (#505835), anti-human LFA-1 (#363404), anti-human CD11a (#301208), anti-human CD18 (#302114), anti-human CD25 (#302625), and anti-human CD69 (#310920) from BioLegend; anti-mouse CD4 (#17-0042-83), anti-mouse CD4 (#17-0041-83), anti-mouse CD69 (#25-0691-82), anti-mouse TNF (#17-7321-82), anti-mouse IL-2 (#12-7021-82), anti-human CD4 (#17-0048-2), and anti-human CD8 (#11-0088-41) from eBioscience.
The antibodies used for immunoprecipitation and immunoblotting include: anti-Cdc7 (#ab10535), anti-Dbf4 (#ab124707), anti-RNA polymerase II (#ab817) and anti-RNA polymerase II (phospho S2, # ab193468) from Abcam; anti-PLCγ (#610027) and anti-ERK (#610031) from BD Biosciences; anti-LAT (#641102) from BioLegend; anti-GAPDH (#2118), anti-pPLCγ (#2821), anti-ZAP70 (#2709), anti-pZAP70 (#2717), anti-pERK (#4370), anti-pLAT (#3584), anti-pLck (#6943), and anti-NF-κB (#3035) from Cell Signaling; anti pMCM2 S40 (#GTX62847) from GeneTex; anti-MCM2 (#MA5-15895) from Pierce; and anti-Lck (#sc-433) from Santa Cruz.

Chen E.W., Tay N.Q., Brzostek J., Gascoigne N.R, & Rybakin V. (2019). A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Frontiers in Immunology, 10, 1718.

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Isolation and Characterization of Th17 Cells

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The isolation of PBMC was performed as previously described [34 (link)]. Staining markers were identified with relevant fluorochrome-conjugated anti-mouse-CD4, anti-mouse-IL-17A, anti-human-CD3, anti-human-CD8, and anti-human-IL-17A mAb (eBioscience,) or Phospho-p38 MAPK Rabbit mAb and anti-rabbit IgG (CST). Intracellular staining for Th17 cells or phospho-p38 MAPK was performed as previously described [16 (link)]. The immunostained cells were analyzed using a FACS Calibur flow cytometer (BD Biosciences).

Tang X., Tian J., Ma J., Wang J., Qi C., Rui K., Wang Y., Xu H., Lu L, & Wang S. (2015). GITRL modulates the activities of p38 MAPK and STAT3 to promote Th17 cell differentiation in autoimmune arthritis. Oncotarget, 7(8), 8590-8600.

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