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2 protocols using mouse anti rat tfr1

1

Iron Homeostasis Regulation in Mouse Macrophages

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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, United States. Mouse anti-rat TfR1 (transferrin receptor 1) and fetal bovine serum (FBS) were purchased from Invitrogen, Carlsbad, CA, United States; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, United States; rabbit polyclonal anti-Ft-L and anti-DMT1 (divalent metal transporter 1, SLC11A2) from Protein-tech, Chicago, IL, United States; and rabbit polyclonal anti-phospho-STAT3 (Tyr705) and mouse monoclonal anti-STAT3 both from Cell Signaling Technology, Inc., Danvers, MA, United States. Recombined mouse IL-6 protein was obtained from Gene Script, Piscataway, NJ, United States; IL-6 enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems, Minneapolis, MN, United States; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies from LI-COR Bio Sciences, Lincoln, NE, United States; TRIzol reagent from Life Technologies, Carlsbad, CA, United States; AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, United States; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, United States; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.
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2

Molecular Mechanisms of Iron Homeostasis

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Unless otherwise stated, all chemicals, including Lipopolysaccharides (LPS) and mouse monoclonal anti-β-actin antibody, were obtained from Sigma-Aldrich Chemical Co., St.
Louis, MO, USA. The mouse anti-ratTfR1 was purchased from Invitrogen, Carlsbad, CA, USA, rabbit polyclonal anti-mouse DMT1 from Alpha Diagnostic International Company, San Antonio, TX, USA, rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, USA; rabbit polyclonal anti-Ft-L from Proteintech, Chicago, IL, USA; mouse monoclonal anti-IRP1 from Abcam, San Francisco, CA, USA; rabbit monoclonal anti-p-JAK2 (phospho-Janus Kinase 2), rabbit monoclonal anti-JAK2, rabbit polyclonal anti-phospho-STAT3 (Tyr705) and mouse monoclonal anti-STAT3 both from Cell Signaling
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