Hydrogen peroxide

Manufactured by AppliChem

Sourced in Germany

Hydrogen peroxide is a colorless, slightly viscous liquid chemical compound with the formula H2O2. It is a strong oxidizing agent used in various industrial and laboratory applications.

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Lab products found in correlation

4 protocols using hydrogen peroxide

Evaluating DNA Damage in Keratinocytes

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Primary human keratinocytes were seeded in 96-well plates at a density of 10 × 10³ cells/well in KGM Gold (Lonza) and preincubated overnight at 37°C/5% CO₂. Cells were then treated with 10 μM and 20 µM DHM for 72 h at 37°C/5% CO₂. DNA double strand breaks were induced in positive control wells with 250 µM hydrogen peroxide (AppliChem) for 15 min. Cells were then fixed with formaldehyde followed by immunostaining with Anti-Phospho-H2A.X-AlexaFluor⁽™⁾555 antibody (Merck Millipore) and counterstaining with Hoechst 33,342 (ThermoFisher). Image acquisition and analysis was performed on the semi-automated high-content imaging system scanR (Olympus).

Falckenhayn C., Bienkowska A., Söhle J., Wegner K., Raddatz G., Kristof B., Kuck D., Siegner R., Kaufmann R., Korn J., Baumann S., Lange D., Schepky A., Völzke H., Kaderali L., Winnefeld M., Lyko F, & Grönniger E. (2024). Identification of dihydromyricetin as a natural DNA methylation inhibitor with rejuvenating activity in human skin. Frontiers in Aging, 4, 1258184.

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Comprehensive Reagent Procurement for Analytical Assays

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Folin–Ciocalteu reagent, gallic acid, phosphoric acid (85%), o-diasidine, peroxidase from Horseradish Type II (150–250 U/mg), and iodine (I) were obtained from Sigma-Aldrich (St Louis, MO, USA). Sodium carbonate solution, Coomassie Brilliant Blue G-250, and ethanol (95%) were purchased from Fluka Analytical (Buchs, Switzerland). Bovine serum albumin was obtained from Biowest (Nuaillé, France). Sodium chloride and Acetate trihydrate sodium was purchased from Merck (Darmstadt, Germany). Glacial acetic acid was purchased from BetaHem, (Belgrade, Serbia). Hydrochloric acid—VWR was obtained from Chemicals (Radnor, PA, USA). Starch was purchased from CarloERBA, (Val-de-Reuil, France). Potassium iodide was purchased from Fisher Scientific (Loughborough, UK). Hydrogen peroxide was obtained from AppliChem GmbH (Darmstadt, Germany).

Stanković M., Prokopijević M., Šikoparija B., Nedić N., Andrić F., Polović N., Natić M, & Radotić K. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. Foods, 12(3), 629.

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Synthesis and Characterization of Noble Metal Nanoparticles

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Analytical grade silver nitrate (puriss. p.a., Sigma-Aldrich), sodium chloride (Reag. Ph. Eur., Merck), sodium hydroxide (50% w/w solution, Fluka Analytical), hydrogen peroxide (10% w/w solution, AppliChem), sodium citrate dihydrate (99%, Sigma-Aldrich), hydroxylamine hydrochloride (99%, Sigma-Aldrich), magnesium sulfate (99%, Sigma-Aldrich), calcium nitrate tetrahydrate (99%, Sigma-Aldrich), potassium iodide (99%, Sigma-Aldrich), crystal violet chloride (Reag. Ph. Eur., Merck), 9-aminoacridine hydrochloride monohydrate (for synthesis, Merck) and rhodamine 6G (99%, Sigma-Aldrich) were dissolved in ultrapure water (Direct-Q 3 UV, Millipore, resistivity higher than 18 MΩ).

Leopold N., Stefancu A., Herman K., Tódor I.S., Iancu S.D., Moisoiu V, & Leopold L.F. (2018). The role of adatoms in chloride-activated colloidal silver nanoparticles for surface-enhanced Raman scattering enhancement. Beilstein Journal of Nanotechnology, 9, 2236-2247.

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Analytical Reagents for Organic Synthesis

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Petroleum ether (40-60°C; 95%) was purchased from Acros Organics (Geel, Belgium). 2,3-Hexanedione (94%) and 2,4,5-trimethyl oxazole (97%) were obtained from Alfa Aesar (Karlsruhe, Germany).
Benzaldehyde (99%) and hydrogen peroxide (30%) were obtained from AppliChem (Darmstadt, Germany). Acetic acid (100%), chloroform-d (99.8 atom% D, with 0.03 vol% TMS, stabilized with Ag), diethyl ether (99.5%), iron(II) sulphate heptahydrate (99.5%), sodium carbonate decahydrate (98%), sodium sulphate (p.a.) and sulfuric acid (96%) were purchased from Carl Roth (Karlsruhe, Germany). Pentanal (97%) was obtained from Fisher Scientific (Darmstadt, Germany), Silica gel 60 was purchased from Macherey-Nagel (Düren, Germany) and 2,3-butanedione (97%) from Merck (Darmstadt, Germany). Acetoin (96%), 2,3-pentanedione (97%), 2,3,5,6-tetramethylpyrazine (98%) and 2,3,5-trimethylpyrazine (99%) were purchased from Sigma-Aldrich (Taufkirchen, Germany). 5-Ethyl-2,3-dimethylpyrazine (98%) was obtained from TCI (Eschborn, Germany). Butanal (97%) and propanal (97%) were purchased from Th. Geyer (Renningen, Germany) and ethyl acetate (98%) from VWR (Darmstadt, Germany). Helium (5.0) was obtained from Praxair (Düsseldorf, Germany) and nitrogen (5.0) from Air Liquide (Düsseldorf, Germany). All numbers given in parentheses represent the minimum purity.

Birk F., Brescia F.F., Fraatz M.A., Pelzer R., & Zorn H. (2021). Aroma active alkylated pyrazines are produced by Basfia succiniciproducens as by‐products of succinic acid production. Flavour and Fragrance Journal, 36(5), 605-612.

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