αS and TDP-43PrLD were fluorescently labeled using Hilyte Flour 532/647 succinimidyl ester (Anaspec) and Hilyte Flour 647, respectively. Proteins were incubated for 16 hours at 4 °C with a 3 M excess of dye in 20 mM MES solution pH 6.0. TDP-43PrLD and αS were eluted in sterile 20 mM MES buffer pH 6.0 using two cycles of the Sephadex G-25 Spin Trap desalting column (Cytiva). For confocal imaging, TDP-43PrLd fluorescently labeled using Hilyte Flour 647 succinimidyl ester (Anaspec) and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec) were used at 1–2% of final protein concentration.
Hilyte flour 647
Manufactured by AnaSpec
Hilyte Flour 647 is a fluorescent dye that can be used for labeling and detection in various applications. It has an excitation maximum at 650 nm and an emission maximum at 665 nm, making it suitable for use with red-fluorescent detection systems. The dye can be conjugated to proteins, nucleic acids, and other biomolecules.
Automatically generated - may contain errors
2 protocols using hilyte flour 647
1
Fluorescent Labeling of αS and TDP-43PrLD
2
Fluorescent Labeling of αS and TDP-43PrLD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
αS and TDP-43PrLD were fluorescently labeled using Hilyte Flour 532/647 succinimidyl ester (Anaspec) and Hilyte Flour 647, respectively. Proteins were incubated for 16 hours at 4 °C with a 3 M excess of dye in 20 mM MES solution pH 6.0. TDP-43PrLD and αS were eluted in sterile 20 mM MES buffer pH 6.0 using two cycles of the Sephadex G-25 Spin Trap desalting column (Cytiva). For confocal imaging, TDP-43PrLd fluorescently labeled using Hilyte Flour 647 succinimidyl ester (Anaspec) and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec) were used at 1-2% of final protein concentration.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!