Maldi tof ms performance

All ¹H and ¹³C NMR spectra were recorded in DMSO-d₆ solutions at 298 K using a Bruker (400 MHz) spectrometer with tetramethylsilane as an internal standard. Mass spectra were recorded on matrix-assisted laser desorption ionization time-of-flight MS (MALDI-TOF MS), Performance (Shimadzu, Japan). Perkin Elmer CE-440 was used for micro elemental analyses. Infrared spectra were recorded by Perkin Elmer (Spectrum 100) FT-IR Spectrometer. UV-vis spectra were recorded using Perkin Elmer (Lambda 750) UV/Vis/NIR Spectrometer in ethyl acetate solvent. Fluorescence emission, quantum yield, and lifetime measurements were performed by SHIMADZU Spectro fluorophotometer RF-6000. Aggregations of HQTPE were examined by scanning electron microscopy (SEM) by a Hitachi SU-8010 SEM instrument fitted with a field-emission source and operating at 10 kV. Samples were prepared for SEM by mounting a portion of the cover slide on a silicon stub. Electrochemical measurements were performed on a CH-Instruments Model CHI660D using a one-compartment cell under air atmosphere. The three-electrode system consisted of a platinum counter electrode, an Ag/AgCl reference electrode, and a platinum working electrode.

All of the solvents and chemicals were purchased in analytical grade and were used as received. Column chromatography used 300–400 mesh silica gels. Ultrapure water was produced by a Milli-Q Direct 16 system of Millipore. UV-Vis absorption spectra were gained on a Shimadzu UV-2550 spectrophotometer (Shimadzu, Kyoto, Japan). Fluorescence spectra were obtained on a Cary Eclipse fluorescence spectrophotometer from Agilent. ¹H- and ¹³C-NMR spectra were recorded with a Mercury plus instrument at 400 and 100 MHz by using DMSO-d₆ as the solvents. MS spectra were recorded on a MALDI-TOF MS Performance (Shimadzu, Japan).