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Uv 2550 pc double beam spectrophotometer

Manufactured by Shimadzu

The UV-2550 PC double-beam spectrophotometer is a versatile laboratory instrument designed to measure the absorbance or transmittance of light in ultraviolet (UV) and visible (VIS) wavelength ranges. It features a double-beam optical system, allowing simultaneous measurement of a sample and a reference. The UV-2550 PC provides precise and reliable data for a wide range of analytical applications.

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3 protocols using uv 2550 pc double beam spectrophotometer

1

Reflective UV-Vis Spectroscopy of Water-Jet Rewritable Paper

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Absorption spectra were measured using a Shimadzu UV-2550 PC double-beam spectrophotometer. Reflective UV–Vis spectroscopy of water-jet rewritable paper (WJRP) before and after addition of water and in situ kinetic measurement was tested via reflective mode of integrating sphere on Analitik Jena Specord®210 plus UV/VIS spectrophotometer, using BaSO4 as background, path length was 1 cm. The writing-erasing cycles experiment for WJRP was recorded by Maya 2000PRO fibre optical spectrometer with Ocean DH-2000-BAL UV-Vis-NIR light source. CIE L*, a*, b* was measured by X-rite spectrodensitometer. X-ray diffraction data was recorded on a Rigaku RAXIS-PRID diffractometer using the ω-scan mode with graphite monochromator Mo·Kα radiation (λ = 0.71073 Å). 1H NMR (500 MHz) and 13C NMR (125 MHz) spectra were recorded on a Bruker AVANCE500 at room temperature. LC-HRMS analysis was performed on an Agilent 1290-micro TOF-Q II mass spectrometer.
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2

Spectroscopic Characterization of Polymers

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Absorption spectra were measured using a Shimadzu UV-2550 PC double-beam spectrophotometer. Absorption spectra of solids were measured via the reflective mode of the integrating sphere on an Analytik Jena Specord®210 plus UV/Vis spectrophotometer, using BaSO4 as background and path length of 1 cm. The fluorescence quantum yields (QYs) were measured on a FLS 920 lifetime and steady-state spectrometer. Steady-state fluorescence spectra were measured using a Shimadzu RF-5301 PC spectrophotometer. 1H NMR (500 MHz) and 13C NMR (126 MHz) spectra were recorded on a Bruker AVANCE 500 at room temperature. LC-HRMS analysis was performed on an Agilent 1290-Micro TOF-Q II mass spectrometer. Mn and Mw/Mn (Ð) of poly(AM-Rhx co NIPAMy)s were measured using PL-GPC 220 high-temperature gel permeation chromatography (GPC) with Agilent PLgel MIXED-B (300 × 7.5 mm, 10 μm), taking DMF as the mobile phase with flow rate of 1.0 mL min−1 at 80 °C, and using RI as the detector and polystyrene (PS) as calibration standard.
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3

Comprehensive NMR and Mass Spectroscopy Analysis

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1H NMR (500, 600 MHz)
and 13C NMR (126, 150 MHz) spectra were recorded on a Bruker
AVANCE500 (or AVANCE 600) using tetramethylsilane (TMS) as the reference
standard at room temperature. In 1H NMR spectra, chemical
shifts (parts per million) were referenced to residual solvent protons
(3.31 ppm in MeOD). In 13C NMR spectra, chemical shifts
(parts per million) were referenced to the carbon signal of the deuterated
solvent (49.0 ppm in MeOD). High resolution electrospray ionization
mass spectrometry analysis was performed on an Agilent 1290-micrOTOF-Q
II mass spectrometer. The UV–vis absorption spectra were measured
from 230 to 800 nm at a scan rate of 5 nm/s using a Shimadzu UV-2550
PC double-beam spectrophotometer with a path length of 1 cm. The solutions
for UV–vis measurements were prepared with a concentration
of 1 × 10–4 M, unless otherwise specified.
The kinetics was measured on an Analitik Jena Specord 210 plus UV–vis
spectrophotometer.
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