Mouse corneas were harvested at indicated time points (n = 3/time point/group), flash frozen in liquid nitrogen, and homogenized in 400 µL of PBS with 1 mM PMSF, 1 µg/mL aprotinin, and 10 µg/mL leupeptin (Sigma-Aldrich, St. Louis, MO). Lysates were centrifuged at 10,000 × g for 10 min at 4°C, and the supernatants used for ELISA. Protein detection for mouse CXCL1, CXCL2, CCL2, and IL-6, and human CXCL8, was performed by sandwich ELISAs (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions. Each sample and standard was analyzed in duplicate. The plates were read on a microplate reader (Molecular Devices, Sunnyvale, CA) and analyzed using SOFTmax software SpectraMax M2 (Molecular Devices).
Softmax software spectramax m2
Manufactured by Molecular Devices
The SOFTmax software SpectraMax M2 is a microplate reader software designed to work with the SpectraMax M2 spectrophotometer. The software provides control and data analysis functionality for the device.
Automatically generated - may contain errors
2 protocols using softmax software spectramax m2
1
Quantifying Cytokine Levels in Mouse Corneas
2
Quantifying Cytokine Levels in Mouse Corneas
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Mouse corneas were harvested at indicated time points (n = 3/time point/group), flash frozen in liquid nitrogen, and homogenized in 400 µL of PBS with 1 mM PMSF, 1 µg/mL aprotinin, and 10 µg/mL leupeptin (Sigma-Aldrich, St. Louis, MO). Lysates were centrifuged at 10,000 × g for 10 min at 4°C, and the supernatants used for ELISA. Protein detection for mouse CXCL1, CXCL2, CCL2, and IL-6, and human CXCL8, was performed by sandwich ELISAs (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions. Each sample and standard was analyzed in duplicate. The plates were read on a microplate reader (Molecular Devices, Sunnyvale, CA) and analyzed using SOFTmax software SpectraMax M2 (Molecular Devices).
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