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P27pt198

Manufactured by R&D Systems
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The P27pT198 is a laboratory equipment product. It is designed for specific scientific applications. The core function of this product is to perform precise measurements and analyses. For detailed information on its intended use and capabilities, please consult the product specifications or technical documentation.

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Lab products found in correlation

Anti pakt s473, by Cell Signaling Technology (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) Pab c 19, by Santa Cruz Biotechnology (2 mentions) Ppdk1 s241, by Cell Signaling Technology (2 mentions) P stat3 y705, by Cell Signaling Technology (2 mentions)

2 protocols using p27pt198

1

Analyzing Protein Signaling Cascades with Western Blotting

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Westerns were as described25 (link). Antibodies used in Westerns: anti- pAKT S473, AKT, pPDK1 S241, PDK1, pSTAT3 Y705 and STAT3 were from Cell Signaling; for E-Cadherin, N-Cadherin and p27 from Transduction Labs and for p27pT157 and p27pT198 from R&D Systems. p27 immunoprecipitation used PAb C-19 from Santa Cruz Biotechnology.
Zhao D., Besser A.H., Wander S.A., Sun J., Zhou W., Wang B., Ince T., Durante M.A., Guo W., Mills G., Theodorescu D, & Slingerland J. (2015). Cytoplasmic p27 promotes epithelial–mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 34(43), 5447-5459.
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2

Western Blot Analysis of AKT, STAT3, and p27 Signaling

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Western blots were performed as described.25 (link) Antibodies used in western blots were as follows: anti-pAKT S473, AKT, pPDK1 S241, PDK1, pSTAT3 Y705 and STAT3 (from Cell Signaling); for E-cadherin, N-cadherin and p27 from Transduction Labs; and for p27pT157 and p27pT198 from R&D Systems. p27 immunoprecipitation used PAb C-19 from Santa Cruz Biotechnology.
Zhao D., Besser A.H., Wander S.A., Sun J., Zhou W., Wang B., Ince T., Durante M.A., Guo W., Mills G., Theodorescu D, & Slingerland J. (2015). Cytoplasmic p27 promotes epithelial–mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 34(43), 5447-5459.
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